A novel Gram-stain negative, red, rod-shaped, non-motile and aerobic bacterial strain, designated Sy30T, was isolated from dry soils of an abandoned marine saltern at Weihai, China. 16S rRNA sequence analysis indicated that strain Sy30T belongs to the genus Pontibacter in the family Cytophagaceae, with sequence similarities ranging from 93.3 to 96.4 % with other type species of the genus Pontibacter. The predominant cellular fatty acids were iso-C15:0 and summed feature 4 (iso-C17:1 I and/or anteiso-C17:1 B). The major menaquinone was MK-7. The major polyamine was sym-homospermidine. The DNA G+C content was 47.7 mol%. The major polar lipids consisted of phosphatidylethanolamine, phosphoaminolipid and two unidentified polar lipid. Based on phenotypic, chemotaxonomic and phylogenetic analysis, strain Sy30T represents a novel species of the genus Pontibacter in the family Cytophagaceae, phylum Bacteroidetes, for which the name Pontibacter locisalis sp. nov. is proposed. The type strain is Sy30T (=KCTC 42498T = CICC AB 2015060T).
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This work was supported by the National Natural Science Foundation of China (31370057, 31370108), National Science and Technology Major Project of China (2013ZX10004217) and 2013 Shandong Provincial Second Group Projects on Resource Platforms for Marine Economic and Innovative Development Regions: Marine Microorganisms Preservation Platform (2150299). We thank China Center for type Culture Collection (CCTCC) for kindly providing the type strains, Pontibacter xinjiangensis CCTCC AB 207200T and Pontibacter korlensis CCTCC AB 206081T.
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Zhou, YX., Xie, ZH., Zhao, JX. et al. Pontibacter locisalis Sy30T sp. nov. isolated from soil collected from an abandoned saltern. Antonie van Leeuwenhoek 109, 415–420 (2016). https://doi.org/10.1007/s10482-016-0646-0
- Pontibacter locisalis
- 16S rRNA gene sequence
- Polyphasic taxonomy