Thalassotalea sediminis sp. nov., isolated from coastal sediment

Abstract

A novel Gram-stain negative, facultatively anaerobic, rod-shaped and yellowish-white-pigmented marine bacterium, designated strain N211T, was isolated from marine sediment obtained off the coastal area of Weihai, China. The cells are approximately 0.4–0.9 × 1.8–3.5 μm in size and motile by means of a polar flagellum. The strain grows optimally at 33 °C, pH 7.5–8.5 and in the presence of 3.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences indicated that strain N211T fell within the clade comprising the type strains of species of the genus Thalassotalea. Strain N211T was most closely related to Thalassotalea ganghwensis DSM 15355T (96.4 %) based on the 16S rRNA gene sequence, and shared 94.4–96.4 % similarity with type strains of all members of the genus Thalassotalea. The predominant isoprenoid quinone of strain N211T was identified as ubiquinone-8 (Q-8). The major polar lipids were found to be phosphatidylethanolamine, phosphatidylglycerol and an unidentified lipid. C17:1 w8c, summed feature 3 (C16:1 w7c and/or iso-C 15:0 2-OH) and C16:0 were found to be main cellular fatty acids. The G+C content of the genomic DNA is 39.1 mol%. It is evident from phenotypic data and phylogenetic inference that strain N211T represents a novel species of the genus Thalassotalea, for which the name T. sediminis sp. nov. is proposed. The type strain is N211T (=KCTC 42588T = MCCC 1H00116T).

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31370057, 31290231), National Science and Technology Major Project of China (2016ZX10004217) and the 2013 Shandong Provincial Second Group Projectson Resource Platforms for Marine Economic and Innovative Development Regions: Marine Microorganisms Preservation Platform (2150299).

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Correspondence to Zong-Jun Du.

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The GenBank accession number for the 16S rRNA gene sequence of T. sediminis N211T is KT224448.

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Xu, ZX., Lu, DC., Liang, QY. et al. Thalassotalea sediminis sp. nov., isolated from coastal sediment. Antonie van Leeuwenhoek 109, 371–378 (2016). https://doi.org/10.1007/s10482-015-0639-4

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Keywords

  • Thalassotalea sediminis sp. nov.
  • 16S rRNA gene
  • Polyphasic taxonomy
  • Marine sediment