A novel Gram-stain negative, facultatively anaerobic, rod-shaped and yellowish-white-pigmented marine bacterium, designated strain N211T, was isolated from marine sediment obtained off the coastal area of Weihai, China. The cells are approximately 0.4–0.9 × 1.8–3.5 μm in size and motile by means of a polar flagellum. The strain grows optimally at 33 °C, pH 7.5–8.5 and in the presence of 3.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences indicated that strain N211T fell within the clade comprising the type strains of species of the genus Thalassotalea. Strain N211T was most closely related to Thalassotalea ganghwensis DSM 15355T (96.4 %) based on the 16S rRNA gene sequence, and shared 94.4–96.4 % similarity with type strains of all members of the genus Thalassotalea. The predominant isoprenoid quinone of strain N211T was identified as ubiquinone-8 (Q-8). The major polar lipids were found to be phosphatidylethanolamine, phosphatidylglycerol and an unidentified lipid. C17:1 w8c, summed feature 3 (C16:1 w7c and/or iso-C 15:0 2-OH) and C16:0 were found to be main cellular fatty acids. The G+C content of the genomic DNA is 39.1 mol%. It is evident from phenotypic data and phylogenetic inference that strain N211T represents a novel species of the genus Thalassotalea, for which the name T. sediminis sp. nov. is proposed. The type strain is N211T (=KCTC 42588T = MCCC 1H00116T).
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Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917
CLSI (2012) Performance standards for antimicrobial susceptibility testing; 22nd informational supplement m100-s22. Clinical and Laboratory Standards Institute, Wayne
Cowan ST, Steel KJ (1965) Manual for the identification of medical bacteria. Cambridge University Press, London
Du ZJ, Wang Y, Dunlap C, Rooney AP, Chen GJ (2014) Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov. Int J Syst Evol Microbiol 64:1690–1696
Hosoya S, Adachi K, Kasai H (2009) Thalassomonas actiniarum sp. nov. and Thalassomonas haliotis sp. nov., isolated from marine animals. Int J Syst Evol Microbiol 59:686–690
Hou TT, Liu Y, Zhong ZP, Liu HC, Liu ZP (2015) Thalassotalea marina sp. nov., isolated from a marine recirculating aquaculture system, reclassification of Thalassomonas eurytherma as Thalassotalea eurytherma comb. nov. and emended description of the genus Thalassotalea. Int J Syst Evol Microbiol. doi:10.1099/ijsem.0.000637
Ivanova EP, Flavier S, Christen R (2004) Phylogenetic relationships among marine Alteromonas-like proteobacteria: emended description of the family Alteromonadaceae and proposal of Pseudoalteromonadaceae fam. nov., Colwelliaceae fam. nov., Shewanellaceae fam. nov., Moritellaceae fam. nov., Ferrimonadaceae fam. nov., Idiomarinaceae fam. nov. and Psychromonadaceae fam. nov. Int J Syst Evol Microbiol 54:1773–1788
Jean WD, Shieh WY, Liu TY (2006) Thalassomonas agarivorans sp. nov., a marine agarolytic bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the genus Thalassomonas. Int J Syst Evol Microbiol 56:1245–1250
Jung YT, Park S, Yoon JH (2014) Thalassomonas fusca sp. nov., a novel gammaproteobacterium isolated from tidal flat sediment. Antonie Van Leeuwenhoek 105:81–87
Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence data-base with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721
Liu QQ, Wang Y, Li J, Du ZJ, Chen GJ (2014) Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans. Int J Syst Evol Microbiol 64:2204–2209
Macian MC, Ludwig W, Schleifer KH, Garay E, Pujalte MJ (2001) Thalassomonas viridans gen. nov., sp. nov., a novel marine cproteobacterium. Int J Syst Evol Microbiol 51:1283–1289
Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167
Park S, Choi WC, Oh TK, Yoon JH (2011) Thalassomonas agariperforans sp. nov., an agarolytic bacterium isolated from marine sand. Int J Syst Evol Microbiol 61:2573–2576
Park S, Jung YT, Kang CH, Park JM, Yoon JH (2014) Thalassotalea ponticola sp. nov., isolated from seawater, reclassification of Thalassomonas fusca as Thalassotalea fusca comb. nov. and emended description of the genus Thalassotalea. Int J Syst Evol Microbiol 64:3676–3682
Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI technical note 101. MIDI Inc, Newark
Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general, molecular bacteriology. American Society for Microbiology, Washington, DC, pp 607–654
Stackebrandt E, Goebel BM (1994) Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849
Sun C, Huo YY, Liu JJ, Pan J, Qi YZ, Zhang XQ, Zhang Y, Zheng G, Wu M (2014) Thalassomonas eurytherma sp. nov., a marine proteobacterium. Int J Syst Evol Microbiol 64:2079–2083
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882
Thompson FL, Barash Y, Sawabe T, Sharon G, Swings J, Rosenberg E (2006) Thalassomonas loyana sp. nov., a causative agent of the white plague-like disease of corals on the Eilat coral reef. Int J Syst Evol Microbiol 56:365–368
Tindall BJ (1990a) A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130
Tindall BJ (1990b) Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Letts 66:199–202
Tindall BJ, Sikorski J, Smibert RM, Kreig NR (2007) Phenotypic characterization and the principles of comparative systematics. In: Methods for general and molecular microbiology 3rd edn: 330–393
Yi H, Bae KS, Chun J (2004) Thalassomonas ganghwensis sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 54:377–380
Zhang Y, Tang K, Shi X, Zhang XH (2014) Description of Thalassotalea piscium gen. nov., sp. nov., isolated from flounder (Paralichthys olivaceus), reclassification of four species of the genus Thalassomonas as members of the genus Thalassotalea gen. nov. and emended description of the genus Thalassomonas. Int J Syst Evol Microbiol 64:1223–1228
This work was supported by the National Natural Science Foundation of China (31370057, 31290231), National Science and Technology Major Project of China (2016ZX10004217) and the 2013 Shandong Provincial Second Group Projectson Resource Platforms for Marine Economic and Innovative Development Regions: Marine Microorganisms Preservation Platform (2150299).
The GenBank accession number for the 16S rRNA gene sequence of T. sediminis N211T is KT224448.
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Xu, ZX., Lu, DC., Liang, QY. et al. Thalassotalea sediminis sp. nov., isolated from coastal sediment. Antonie van Leeuwenhoek 109, 371–378 (2016). https://doi.org/10.1007/s10482-015-0639-4