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Thalassotalea sediminis sp. nov., isolated from coastal sediment

Antonie van Leeuwenhoek volume 109, pages 371–378 (2016)Cite this article

Abstract

A novel Gram-stain negative, facultatively anaerobic, rod-shaped and yellowish-white-pigmented marine bacterium, designated strain N211T, was isolated from marine sediment obtained off the coastal area of Weihai, China. The cells are approximately 0.4–0.9 × 1.8–3.5 μm in size and motile by means of a polar flagellum. The strain grows optimally at 33 °C, pH 7.5–8.5 and in the presence of 3.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences indicated that strain N211T fell within the clade comprising the type strains of species of the genus Thalassotalea. Strain N211T was most closely related to Thalassotalea ganghwensis DSM 15355T (96.4 %) based on the 16S rRNA gene sequence, and shared 94.4–96.4 % similarity with type strains of all members of the genus Thalassotalea. The predominant isoprenoid quinone of strain N211T was identified as ubiquinone-8 (Q-8). The major polar lipids were found to be phosphatidylethanolamine, phosphatidylglycerol and an unidentified lipid. C17:1 w8c, summed feature 3 (C16:1 w7c and/or iso-C 15:0 2-OH) and C16:0 were found to be main cellular fatty acids. The G+C content of the genomic DNA is 39.1 mol%. It is evident from phenotypic data and phylogenetic inference that strain N211T represents a novel species of the genus Thalassotalea, for which the name T. sediminis sp. nov. is proposed. The type strain is N211T (=KCTC 42588T = MCCC 1H00116T).

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References

  • Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917

    Article  CAS  PubMed  Google Scholar 

  • CLSI (2012) Performance standards for antimicrobial susceptibility testing; 22nd informational supplement m100-s22. Clinical and Laboratory Standards Institute, Wayne

    Google Scholar 

  • Cowan ST, Steel KJ (1965) Manual for the identification of medical bacteria. Cambridge University Press, London

    Google Scholar 

  • Du ZJ, Wang Y, Dunlap C, Rooney AP, Chen GJ (2014) Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov. Int J Syst Evol Microbiol 64:1690–1696

    Article  CAS  PubMed  Google Scholar 

  • Hosoya S, Adachi K, Kasai H (2009) Thalassomonas actiniarum sp. nov. and Thalassomonas haliotis sp. nov., isolated from marine animals. Int J Syst Evol Microbiol 59:686–690

    Article  CAS  PubMed  Google Scholar 

  • Hou TT, Liu Y, Zhong ZP, Liu HC, Liu ZP (2015) Thalassotalea marina sp. nov., isolated from a marine recirculating aquaculture system, reclassification of Thalassomonas eurytherma as Thalassotalea eurytherma comb. nov. and emended description of the genus Thalassotalea. Int J Syst Evol Microbiol. doi:10.1099/ijsem.0.000637

    Google Scholar 

  • Ivanova EP, Flavier S, Christen R (2004) Phylogenetic relationships among marine Alteromonas-like proteobacteria: emended description of the family Alteromonadaceae and proposal of Pseudoalteromonadaceae fam. nov., Colwelliaceae fam. nov., Shewanellaceae fam. nov., Moritellaceae fam. nov., Ferrimonadaceae fam. nov., Idiomarinaceae fam. nov. and Psychromonadaceae fam. nov. Int J Syst Evol Microbiol 54:1773–1788

    Article  CAS  PubMed  Google Scholar 

  • Jean WD, Shieh WY, Liu TY (2006) Thalassomonas agarivorans sp. nov., a marine agarolytic bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the genus Thalassomonas. Int J Syst Evol Microbiol 56:1245–1250

    Article  CAS  PubMed  Google Scholar 

  • Jung YT, Park S, Yoon JH (2014) Thalassomonas fusca sp. nov., a novel gammaproteobacterium isolated from tidal flat sediment. Antonie Van Leeuwenhoek 105:81–87

    Article  CAS  PubMed  Google Scholar 

  • Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence data-base with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721

    Article  CAS  PubMed  Google Scholar 

  • Liu QQ, Wang Y, Li J, Du ZJ, Chen GJ (2014) Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans. Int J Syst Evol Microbiol 64:2204–2209

    Article  CAS  PubMed  Google Scholar 

  • Macian MC, Ludwig W, Schleifer KH, Garay E, Pujalte MJ (2001) Thalassomonas viridans gen. nov., sp. nov., a novel marine cproteobacterium. Int J Syst Evol Microbiol 51:1283–1289

    Article  PubMed  Google Scholar 

  • Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167

    Article  CAS  Google Scholar 

  • Park S, Choi WC, Oh TK, Yoon JH (2011) Thalassomonas agariperforans sp. nov., an agarolytic bacterium isolated from marine sand. Int J Syst Evol Microbiol 61:2573–2576

    Article  PubMed  Google Scholar 

  • Park S, Jung YT, Kang CH, Park JM, Yoon JH (2014) Thalassotalea ponticola sp. nov., isolated from seawater, reclassification of Thalassomonas fusca as Thalassotalea fusca comb. nov. and emended description of the genus Thalassotalea. Int J Syst Evol Microbiol 64:3676–3682

    Article  PubMed  Google Scholar 

  • Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI technical note 101. MIDI Inc, Newark

    Google Scholar 

  • Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general, molecular bacteriology. American Society for Microbiology, Washington, DC, pp 607–654

    Google Scholar 

  • Stackebrandt E, Goebel BM (1994) Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849

    Article  CAS  Google Scholar 

  • Sun C, Huo YY, Liu JJ, Pan J, Qi YZ, Zhang XQ, Zhang Y, Zheng G, Wu M (2014) Thalassomonas eurytherma sp. nov., a marine proteobacterium. Int J Syst Evol Microbiol 64:2079–2083

    Article  CAS  PubMed  Google Scholar 

  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Thompson FL, Barash Y, Sawabe T, Sharon G, Swings J, Rosenberg E (2006) Thalassomonas loyana sp. nov., a causative agent of the white plague-like disease of corals on the Eilat coral reef. Int J Syst Evol Microbiol 56:365–368

    Article  CAS  PubMed  Google Scholar 

  • Tindall BJ (1990a) A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130

    Article  CAS  Google Scholar 

  • Tindall BJ (1990b) Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Letts 66:199–202

    Article  CAS  Google Scholar 

  • Tindall BJ, Sikorski J, Smibert RM, Kreig NR (2007) Phenotypic characterization and the principles of comparative systematics. In: Methods for general and molecular microbiology 3rd edn: 330–393

  • Yi H, Bae KS, Chun J (2004) Thalassomonas ganghwensis sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 54:377–380

    Article  CAS  PubMed  Google Scholar 

  • Zhang Y, Tang K, Shi X, Zhang XH (2014) Description of Thalassotalea piscium gen. nov., sp. nov., isolated from flounder (Paralichthys olivaceus), reclassification of four species of the genus Thalassomonas as members of the genus Thalassotalea gen. nov. and emended description of the genus Thalassomonas. Int J Syst Evol Microbiol 64:1223–1228

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (31370057, 31290231), National Science and Technology Major Project of China (2016ZX10004217) and the 2013 Shandong Provincial Second Group Projectson Resource Platforms for Marine Economic and Innovative Development Regions: Marine Microorganisms Preservation Platform (2150299).

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Authors and Affiliations

  1. College of Marine Science, Shandong University at Weihai, Weihai, 264209, China

    Zhen-Xing Xu, De-Chen Lu, Qi-Yun Liang, Guan-Jun Chen & Zong-Jun Du

  2. State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, China

    Guan-Jun Chen & Zong-Jun Du

Authors
  1. Zhen-Xing Xu
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  2. De-Chen Lu
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  3. Qi-Yun Liang
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  4. Guan-Jun Chen
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  5. Zong-Jun Du
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Correspondence to Zong-Jun Du.

Additional information

The GenBank accession number for the 16S rRNA gene sequence of T. sediminis N211T is KT224448.

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Xu, ZX., Lu, DC., Liang, QY. et al. Thalassotalea sediminis sp. nov., isolated from coastal sediment. Antonie van Leeuwenhoek 109, 371–378 (2016). https://doi.org/10.1007/s10482-015-0639-4

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  • DOI: https://doi.org/10.1007/s10482-015-0639-4

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