Skip to main content

Description of a taxonomically undefined Sclerotiniaceae strain from withered rotten-grapes

Antonie van Leeuwenhoek volume 109, pages 197–205 (2016)Cite this article

Abstract

A necrotrophic member of the Sclerotiniaceae family (herewith named strain C10) isolated from withered rotten-grapes, is described. Interestingly, the fungus has no defined taxonomic position since it has been impossible to attribute it to an existing genus. Phylogenetic analysis of partial sequences of glyceraldehyde 3-phosphate dehydrogenase (G3PDH), heat shock protein 60 (HSP60) and DNA-directed RNA polymerase II subunit (RPB2), revealed that strain C10 is distantly related to Amphobotrys and Botrytis. This evidence clearly distinguishes this new Sclerotiniaceae member from other taxa of the family. Moreover, its morphological characteristics did not match those of Amphobotrys and Botrytis. Infectivity assays demonstrated that strain C10 could be a potential postharvest pathogen of withered grapes. This study revealed the taxonomic importance of this strain suggesting the existence of a possible new genus, a theory that requires further investigation.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  • Abawi GS, Grogan RG (1979) Epidemiology of diseases caused by Sclerotinia species. Phytopathology 69:899–904

    Article  Google Scholar 

  • Andrew M, Barua R, Short SM, Kohn LM (2012) Evidence for a common toolbox based on necrotrophy in a fungal lineage spanning necrotrophs, biotrophs, endophytes, host generalists and specialists. PlosOne 7:1–14

    Google Scholar 

  • Azzolini M, Tosi E, Faccio S, Lorenzini M, Torriani S, Zapparoli G (2013) Selection of Botrytis cinerea and Saccharomyces cerevisiae strains for the improvement and valorization of Italian passito style wines. FEMS Yeast Res 13:540–552

    Article  CAS  PubMed  Google Scholar 

  • Bolton MD, Thomma BP, Nelson DB (2006) Sclerotinia sclerotiorum (Lib.) de Bary: biology and molecular traits of a cosmopolitan pathogen. Mol Plant Pathol 7:1–16

    Article  CAS  PubMed  Google Scholar 

  • Bullock S, Willetts HJ (1996) Ultrastructural and histochemical studies on mycelial germination of sclerotia of Sclerotinia minor. Mycol Res 100:561–570

    Article  Google Scholar 

  • Hall BH, McMahon RL, Wicks TJ (2002) First report of Sclerotinia sclerotiorum on grape (Vitis vinifera) in South Australia. Australas Plant Pathol 31:417–418

    Article  Google Scholar 

  • Hennebert GL (1973) Botrytis and Botrytis-like genera. Persoonia 7:183–204

    Google Scholar 

  • Holst-Jensen A, Kohn LM, Schumacher T (1997) Nuclear rDNA phylogeny of the Sclerotiniaceae. Mycologia 89:885–899

    Article  CAS  Google Scholar 

  • Holst-Jensen A, Vrålstad T, Schumacher T (2004) Kohninia linnaeicola, a new genus and species of the Sclerotiniaceae pathogenic to Linnaea borealis. Mycologia 96:135–142

    Article  CAS  PubMed  Google Scholar 

  • Latorre BA, Guerrero MJ (2001) First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Chile. Plant Dis 85:1122

    Article  Google Scholar 

  • Li X, Kerrigan J, Chai W, Schnabel G (2012) Botrytis caroliniana, a new species isolated from blackberry in South Carolina. Mycologia 104:650–658

    Article  CAS  PubMed  Google Scholar 

  • Lorenzini M, Zapparoli G (2014) An isolate morphologically and phylogenetically distinct from Botrytis cinerea obtained from withered grapes possibly represents a new species of Botrytis. Plant Pathol 63:1326–1335

    Article  CAS  Google Scholar 

  • Lorenzini M, Azzolini M, Tosi E, Zapparoli G (2013) Post-harvest grape infection of Botrytis cinerea and its interactions with other moulds under withering conditions to produce noble-rotten grapes. J Appl Microbiol 114:762–770

    Article  CAS  PubMed  Google Scholar 

  • Masuya H, Kusunoki M, Kosaka H, Aikawa T (2009) Haradamyces foliicola anam. gen. et sp. nov., a cause of zonate leaf blight disease in Cornus florida in Japan. Mycol Res 113:173–181

    Article  PubMed  Google Scholar 

  • Ordóñez-Valencia C, Ferrera-Cerrato R, Quintanar-Zúñiga RE, Flores-Ortiz CM, Márquez Guzmán GJ, Alarcón A, Larsen J, García-Barradas O (2015) Morphological development of sclerotia by Sclerotinia sclerotiorum: a view from light and scanning electron microscopy. Ann Microbiol 65:765–770

    Article  Google Scholar 

  • Pitt JI, Hocking AD (1997) Fungi and Food Spoilage, 2nd edn. Academic Press, Sydney

    Book  Google Scholar 

  • Saharan GS, Mehta N (2008) Sclerotinia disease of crop plants: biology, ecology and disease management, 1st edn. Springer, The Netherlands

    Book  Google Scholar 

  • Smith EA, Boland GJ (1989) A reliable method for production of apothecia of Sclerotinia sclerotiorum. Can J Plant Pathol 11:45–48

    Article  CAS  Google Scholar 

  • Staats M, van Baarlen P, van Kan JAL (2005) Molecular phylogeny of the plant pathogenic genus Botrytis and the evolution of host specificity. Mol Biol Evol 22:333–346

    Article  CAS  PubMed  Google Scholar 

  • Staats M, van Baarlen P, Schouten A, van Kan JAL, Bakker FT (2007) Positive selection in phytotoxic protein-encoding genes of Botrytis species. Fungal Genet Biol 44:52–63

    Article  CAS  PubMed  Google Scholar 

  • Takahashi Y, Ichihashi Y, Sano T, Harada Y (2005) Monilinia jezoensis sp. nov. in the Sclerotiniaceae, causing leaf blight and mummy fruit disease of Rhododendron kaempferi in Hokkaido, northern Japan. Mycoscience 46:106–109

    Article  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315–322

    Google Scholar 

  • Willetts HJ (1997) Morphology, development and evolution of stromata/sclerotia and macroconidia of the Sclerotiniaceae. Mycol Res 101:939–952

    Article  Google Scholar 

  • Williamson B, Tudzynski B, Tudzynski P, van Kan JAL (2007) Pathogen profile Botrytis cinerea: the cause of grey mould disease. Mol Plant Pathol 8:561–580

    Article  CAS  PubMed  Google Scholar 

  • Zhou YJ, Zhang J, Wang XD, Yang L, Jiang DH, Li GQ, Hsiang T, Zhuang WY (2014) Morphological and phylogenetic identification of Botrytis sinoviticola, a novel cryptic species causing gray mold disease of table grapes (Vitis vinifera) in China. Mycologia 106:43–56

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Affiliations

  1. Dipartimento di Biotecnologie, Università degli Studi di Verona, 37134, Verona, Italy

    Marilinda Lorenzini & Giacomo Zapparoli

Authors
  1. Marilinda Lorenzini
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Giacomo Zapparoli
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Marilinda Lorenzini.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 712 kb)

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Lorenzini, M., Zapparoli, G. Description of a taxonomically undefined Sclerotiniaceae strain from withered rotten-grapes. Antonie van Leeuwenhoek 109, 197–205 (2016). https://doi.org/10.1007/s10482-015-0621-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10482-015-0621-1

Keywords

  • Sclerotiniaceae
  • Phylogenetic analysis
  • Taxonomy
  • Withered grapes