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Enteractinococcus lamae sp. nov. and Enteractinococcus viverrae sp. nov., isolated from animal faeces

Antonie van Leeuwenhoek volume 108, pages 1477–1483 (2015)Cite this article

Abstract

Two novel actinobacteria, designated strains YIM 101617T and YIM 101632T, were isolated from Lama pacos (alpaca) and Viverra zibetha (civet) faeces in Yunnan Wild Animal Park in Yunnan province, southwestern China. Both strains should be placed in genus Enteractinococcus based on phylogenetic analysis. Based on 16S rRNA gene sequence analysis, strain YIM 101617T exhibits high similarity to Enteractinococcus fodinae DSM 22966T (97.70 %) and Enteractinococcus coprophilus YIM 100590T (97.45 %), whilst YIM 101632T exhibits high similarity to Enteractinococcus coprophilus YIM 100590T (97.25 %), and the similarity between YIM 101617T and YIM 101632T is 95.90 %. However, DNA–DNA hybridization values of the two strains with the type strains in the genus Enteractinococcus were low (<70 %). Most morphological and chemotaxonomic characteristics of the two strains were found to be similar to those of species in the genus Enteractinococcus but also some differences were observed. The DNA G+C contents of strains YIM 101617T and YIM 101632T were determined to be 55.9 and 56.4 mol%, respectively. Based on these data, the two strains are concluded to represent two different novel species in the genus Enteractinococcus. The names Enteractinococcus lamae sp. nov. (type strain YIM 101617T=DSM 27612T=CCTCC AB 2013230T) and Enteractinococcus viverrae sp. nov. (type strain YIM 101632T=KCTC 39552T=CCTCC AB 2013280T) are proposed, respectively.

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Acknowledgments

This research was funded by the National Natural Science Foundation of China (No. 31270001), and supported by Program for New Century Excellent Talents in University and Basic Scientific Research Fund of Northeastern University, China (No. N120820002). Thanks for the assistance of Yunnan Wild Animal Park, and the help from Mr. Chun-hua Yang and animal keeper Dr. Jun-long Huang.

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    Authors and Affiliations

    1. Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang, 110819, People’s Republic of China

      Xiu Chen, Cai-Juan Hu, Li Han & Xue-Shi Huang

    2. Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

      Gui-Ding Li, Qin-Yuan Li, Yi Jiang & Cheng-Lin Jiang

    3. Yunnan Wild Animal Park, Kunming, 650218, People’s Republic of China

      Shu-Mei Qiu

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    1. Xiu Chen
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    Correspondence to Yi Jiang or Li Han.

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    Xiu Chen and Gui-Ding Li have contributed equally to this work.

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    Chen, X., Li, GD., Li, QY. et al. Enteractinococcus lamae sp. nov. and Enteractinococcus viverrae sp. nov., isolated from animal faeces. Antonie van Leeuwenhoek 108, 1477–1483 (2015). https://doi.org/10.1007/s10482-015-0603-3

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    Keywords

    • Enteractinococcus lamae sp. nov.
    • Enteractinococcus viverrae sp. nov.
    • 16S rRNA