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Citreicella manganoxidans sp. nov., a novel manganese oxidizing bacterium isolated from a shallow water hydrothermal vent in Espalamaca (Azores)

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Abstract

A Gram-stain negative, non-motile, non-spore forming, aerobic and rod or narrow lemon-shaped bacterial strain, VSW210T, was isolated from surface seawater in a shallow water hydrothermal vent region in Espalamaca (Azores). Strain VSW210T was found to grow optimally at 30 °C, at pH 7 and in the presence of 2–6 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain VSW210T clusters with the type strain Citreicella marina CK-I3-6T (sequence similarity value of 99.6 %), but DNA–DNA hybridization showed DNA–DNA relatedness between the strain VSW210T and C. marina CK-I3-6T to be 55.8 ± 3.2 %. The DNA G+C content of strain VSW210T was determined to be 67.4 mol%. The cellular fatty acid profiles of strain VSW210T was found to contain C18:1 ω7c (80.1 %) and C16:0 (9.2 %). The major polar lipids in strain VSW210T were identified as phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified phospholipid. Strain VSW210T was found to be able to oxidize soluble Mn(II) to insoluble MnO2, which was confirmed with LBB staining. Differential phenotypic properties and genetic uniqueness revealed that this strain VSW210T is distinguishable from other species of the genus Citreicella. On the basis of the data presented, strain VSW210T is considered to represent a novel species of the genus Citreicella, for which the name Citreicella manganoxidans sp. nov. is proposed. The type strain is VSW210T (=KCTC 32497T = MCC 2286T).

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Acknowledgments

The authors thank Dr. N. Ramaiah and Mr. RM. Meena for providing the DNA sequencing facility; Mr. Areef Sardar for SEM analysis. Author Rajasabapathy acknowledges CSIR, New Delhi for a SRF grant. We thank the Department of Science & Technology, Govt. of India for the support through the Indo-Portugal bilateral program. This work was also supported by a CSIR-NIO project ‘PSC0206′. We acknowledge MMRF-COMAPS under ICMAM, MoES through Dr. Shanta Nair, Dr. Anas and Mrs. Vijitha for FAME analysis. IMAR-DOP/UAz is Research and Development Unit #531 and LARSyS—Associated Laboratory # 9 funded by the Portuguese Foundation for Science and Technology (FCT) through PEst project (Pest/OE/EEI/LA0009/2011–2014), and by DRCTC—Regional Government of the Azores through a Pluriannual Funding scheme. This is CSIR-NIO contribution number 5810.

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Authors and Affiliations

  1. Biological Oceanography Division, CSIR-National Institute of Oceanography, Dona Paula, Goa, 403 004, India

    Raju Rajasabapathy & Chellandi Mohandass

  2. NCIM Resource Center, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, Pune, 411 008, India

    Syed Gulam Dastager

  3. Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, Yunnan, People’s Republic of China

    Qing Liu & Wen-Jun Li

  4. IMAR-Department of Oceanography and Fisheries, University of Azores, Rua Prof Frederico Machado, 9901-862, Horta, Portugal

    Ana Colaço

Authors
  1. Raju Rajasabapathy
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  2. Chellandi Mohandass
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  3. Syed Gulam Dastager
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  4. Qing Liu
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  5. Wen-Jun Li
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  6. Ana Colaço
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Correspondence to Chellandi Mohandass.

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Rajasabapathy, R., Mohandass, C., Dastager, S.G. et al. Citreicella manganoxidans sp. nov., a novel manganese oxidizing bacterium isolated from a shallow water hydrothermal vent in Espalamaca (Azores). Antonie van Leeuwenhoek 108, 1433–1439 (2015). https://doi.org/10.1007/s10482-015-0597-x

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