Lentzea guizhouensis sp. nov., a novel lithophilous actinobacterium isolated from limestone from the Karst area, Guizhou, China

Abstract

A novel filamentous actinobacterium, designated strain DHS C013T, was isolated from limestone collected in Guizhou Province, South-west China. Morphological and chemotaxonomic characteristics of the strain support its assignment to the genus Lentzea. Phylogenetic analyses showed that strain DHS C013T is closely related to Lentzea jiangxiensis FXJ1.034T (98.7 % 16S rRNA gene similarity) and Lentzea flaviverrucosa 4.0578T (98.0 % 16S rRNA gene similarity), but it can be distinguished from these strains based on low levels of DNA:DNA relatedness (~44 and ~37 %, respectively). Physiological and biochemical tests also allowed phenotypic differentiation of the novel strain from these closely related species. On the basis of the evidence presented here, strain DHS C013T is concluded to represent a novel species of the genus Lentzea, for which the name Lentzea guizhouensis sp. nov. is proposed. The type strain is DHS C013T (=KCTC 29677T = CGMCC 4.7203T).

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References

  1. Collins MD, Pirouz T, Goodfellow M, Minnikin DE (1977) Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230

    CAS  Article  PubMed  Google Scholar 

  2. Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229

    Article  Google Scholar 

  3. Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376

    CAS  Article  PubMed  Google Scholar 

  4. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–789

    Article  PubMed  Google Scholar 

  5. Gordon RE, Barnett DA, Handerhan JE, Pang CH-N (1974) Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63

    Article  Google Scholar 

  6. Groth I, Schumann P, Rainey FA, Martin K, Schuetze B, Augsten K (1997) Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47:1129–1133

    CAS  Article  PubMed  Google Scholar 

  7. Hasegawa T, Takizawa M, Tanida S (1983) A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322

    CAS  Article  Google Scholar 

  8. He L, Li W, Huang Y, Wang L, Liu Z, Lanoot B, Vancanneyt M, Swings J (2005) Streptomyces jietaisiensis sp nov., isolated from soil in northern China. Int J Syst Evol Microbiol 55:1939–1944

    CAS  Article  PubMed  Google Scholar 

  9. Kelly KL (1964) Color-name charts illustrated with centroid colors. Inter-Society Color Council-National Bureau of Standards, Chicago

    Google Scholar 

  10. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721

    CAS  Article  PubMed  Google Scholar 

  11. Kluge AG, Farris JS (1969) Quantitative phyletics and the evolution of anurans. Syst Biol 18:1–32

    Article  Google Scholar 

  12. Kurup PV, Schmitt JA (1973) Numerical taxonomy of Nocardia. Can J Microbiol 19:1035–1048

    CAS  Article  PubMed  Google Scholar 

  13. Labeda DP (2012) Genus XI. Lentzea Yassin, Rainey, Brzezinka, Jahnke, Weissbrodt, Budzikiewicz, Stackebrandt and Schaal 1995, 1125VP emend. Labeda, Hatano, Kroppenstedt and Tamura 2001, 1049. In: Goodfellow M, Kämpfer P, Busse H-J, Trujillo ME, Suzuki K-I, Ludwig W, Whitman WB (eds) Bergey’s manual of systematic bacteriology, part A, vol 5, 2nd edn. Springer, New York, pp 1379–1383

    Google Scholar 

  14. Labeda DP, Hatano K, Kroppenstedt RM, Tamura T (2001) Revival of the genus Lentzea and proposal for Lechevalieria gen. nov. Int J Syst Evol Microbiol 51:1045–1050

    CAS  Article  PubMed  Google Scholar 

  15. Labeda DP, Donahue JM, Sells SF, Kroppenstedt RM (2007) Lentzea kentuckyensis sp. nov., of equine origin. Int J Syst Evol Microbiol 57:1780–1783

    CAS  Article  PubMed  Google Scholar 

  16. Labeda DP, Goodfellow M, Chun J, Zhi XY, Li WJ et al (2011) Reassessment of the systematics of the suborder Pseudonocardineae: transfer of the genera within the family Actinosynnemataceae Labeda and Kroppenstedt 2000 emend. Zhi et al. 2009 into an emended family Pseudonocardiaceae Embley et al. 1989 emend. Zhi et al. 2009. Int J Syst Evol Microbiol 61:1259–1264

    CAS  Article  PubMed  Google Scholar 

  17. Lechevalier MP, Lechevalier HA (1970) Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443

    CAS  Article  Google Scholar 

  18. Lee SD, Kim ES, Roe JH, Kim J, Kang SO, Hah YC (2000) Saccharothrix violacea sp. nov., isolated from a gold mine cave, and Saccharothrix albidocapillata comb. nov. Int J Syst Evol Microbiol 50:1315–1323

    CAS  Article  PubMed  Google Scholar 

  19. Li X, Zhang L, Ding Y, Gao Y, Ruan J, Huang Y (2012) Lentzea jiangxiensis sp. nov., isolated from acidic soil. Int J Syst Evol Microbiol 62:2342–2346

    CAS  Article  PubMed  Google Scholar 

  20. Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167

    CAS  Article  Google Scholar 

  21. Minnikin DE, O’donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241

    CAS  Article  Google Scholar 

  22. Qin S, Li J, Chen HH, Zhao GZ, Zhu WY, Jiang CL, Xu LH, Li WJ (2009) Isolation, diversity, and antimicrobial activity of rare actinobacteria from medicinal plants of tropical rain forests in Xishuangbanna, China. Appl Environ Microbiol 75:6176–6186

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  23. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  24. Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI technical note 101. MIDI Inc., Newark

    Google Scholar 

  25. Shirling E, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340

    Article  Google Scholar 

  26. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore EC, Murray RGE, Stackebrandt E, Starr MP (1987) Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464

    Article  Google Scholar 

  27. Williams ST, Goodfellow M, Alderson G (1989) Genus Streptomyces Waksman and Henrici 1943, 339AL. In: Williams ST, Sharpe ME, Holt JG (eds) Bergey’s manual of systematic bacteriology, vol 4. Williams & Wilkins, Baltimore, pp 2452–2492

    Google Scholar 

  28. Xie Q, Wang Y, Huang Y, Wu Y, Ba F, Liu Z (2002) Description of Lentzea flaviverrucosa sp. nov. and transfer of the type strain of Saccharothrix aerocolonigenes subsp. staurosporea to Lentzea albida. Int J Syst Evol Microbiol 52:1815–1820

    CAS  PubMed  Google Scholar 

  29. Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ, Chen HH, Xu LH, Jiang CL (2005) Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55:1149–1153

    CAS  Article  PubMed  Google Scholar 

  30. Yassin AF, Rainey F, Brzezinka H, Jahnke KD, Weissbrodt H, Budzikiewicz H, Stackebrandt E, Schaal KP (1995) Lentzea gen. nov., a new genus of the order Actinomycetales. Int J Syst Bacteriol 45:357–363

    CAS  Article  PubMed  Google Scholar 

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Acknowledgments

The authors are grateful to Prof. Ying Huang (State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.) for her kindly providing type strains. This study was jointly supported by the National Key Basic Research Program of China (No. 2013CB956702), the National Natural Science Foundation of China (No. 41373078), and the Natural Science Foundation of Jiangsu Normal University (No. 13XLA02).

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Correspondence to Bin Lian.

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Cheng-Liang Cao and Xiao-Qi Zhou have contributed equally to this work.

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Cao, CL., Zhou, XQ., Qin, S. et al. Lentzea guizhouensis sp. nov., a novel lithophilous actinobacterium isolated from limestone from the Karst area, Guizhou, China. Antonie van Leeuwenhoek 108, 1365–1372 (2015). https://doi.org/10.1007/s10482-015-0589-x

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Keywords

  • Lentzea guizhouensis sp. nov.
  • Lithophilous
  • Karst area
  • Polyphasic taxonomy