Psychrobacter fjordensis sp. nov., a psychrotolerant bacterium isolated from an Arctic fjord in Svalbard

Abstract

A Gram-negative, non-motile, non-spore-forming, psychrotolerant and halotolerant bacterium designated BSw21516BT, was obtained from seawater in Kongsfjorden, a glacial fjord in the Arctic Svalbard and subjected to taxonomic analysis using a polyphasic approach. This bacterium was observed to optimally grow at 25–29 °C; between at 4 and 34 °C, but not at >35 °C; and in the presence of 0–8 % (w/v) NaCl at an optimum concentration of 2–5 % (w/v) NaCl. Strain BSw21516BT was found to contain Ubiquinone-8 (Q-8) as a predominant respiratory lipoquinone and C18:1 ω9c and summed feature 3 (C16:1 ω7c and/or iso-C15:0 2-OH) as predominant cellular fatty acids. Phylogenetic analysis of 16S rRNA and gyrB gene sequences showed that this isolate belongs to the genus Psychrobacter and is closely related to Psychrobacter fozii LMG 21280T, which was isolated from a sediment sample in Antarctica. DNA hybridization experiments revealed a low level of DNA–DNA relatedness (less than 58.6 %) between strain BSw21516BT and its closest relatives. Based on these results a new species Psychrobacter fjordensis sp. nov. is proposed (type strain BSw21516BT = KCTC 42279T = CCTCC AB 2014020T).

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Acknowledgments

We appreciate the assistance of the Chinese Arctic and Antarctic Administration (CAA) who organized the Chinese Arctic Yellow River Station Expedition in 2008. This work was supported by the National Natural Science Foundation of China (Grant Nos. 41076131 and 41476171) and the Chinese Polar Environment Comprehensive Investigation and Assessment Program (Grant No. CHINARE2015-02-01).

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Correspondence to Yin-Xin Zeng.

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Zeng, YX., Yu, Y., Li, HR. et al. Psychrobacter fjordensis sp. nov., a psychrotolerant bacterium isolated from an Arctic fjord in Svalbard. Antonie van Leeuwenhoek 108, 1283–1292 (2015). https://doi.org/10.1007/s10482-015-0580-6

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Keywords

  • Psychrobacter fjordensis sp. nov.
  • Psychrobacter
  • Kongsfjorden
  • Arctic