A rod shaped, Gram-stain positive, non-motile, facultative anaerobic and gelatin hydrolysing bacterium, strain PG1T, was isolated from reclaimed land soil in Kyehwa-do, Republic of Korea. Strain PG1T showed highest 16S rRNA gene sequence similarity (97.4 and 96.5 %, respectively) to Paenibacillus shenyangensis A9T and Paenibacillus hunanensis FeL05T, and clustered closely with the members of the family Paenibacillaceae. DNA–DNA hybridization studies revealed a genomic relatedness of 47 ± 9 % with P. shenyangensis A9T. The predominant fatty acids of strain PG1T were identified to be anteiso-C15:0 (46.7 %) and C16:0 (22.7 %). Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid were found to be the major polar lipids. The genomic DNA G+C content was found to be 47.7 mol%. This polyphasic characterisation of the newly isolated strain PG1T justifies its description as representative of a novel species in the genus Paenibacillus, for which the name Paenibacillus gelatinilyticus sp. nov., (type strain = PG1T = KCTC 33642T = JCM 30624T) is proposed.
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We thank Prof. Aharon Oren for his expert suggestion for correct species epithet and Latin etymology. This work was carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ009801)” Rural Development Administration, Republic of Korea.
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain PG1T is KP231650.
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Padakandla, S.R., Lee, GW. & Chae, JC. Paenibacillus gelatinilyticus sp. nov. a psychrotolerant bacterium isolated from a reclaimed soil and amended description of Paenibacillus shenyangensis . Antonie van Leeuwenhoek 108, 1197–1203 (2015). https://doi.org/10.1007/s10482-015-0574-4