A novel Gram-negative, strictly aerobic, short rod-shaped, non-motile bacterium, designated YIM 101302T, was isolated from the faeces of Rhinoceros unicornis dwelling in the Yunnan Wild Animal Park, Yunnan province, South-West China. The 16S rRNA gene sequence analysis revealed a clear affiliation of strain YIM 101302T to the genus Sphingobacterium. The newly isolated bacterium was found to be closely related to Sphingobacterium composti T5-12T (97.1 % 16S rRNA sequence identity) and Sphingobacterium alimentarium WCC 4521T (95.6 % 16S rRNA sequence identity) forming a distinct clade with these two species. Polar lipids of strain YIM 101302T were identified as phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, phosphatidylinositol, an unidentified aminophospholipid, and three unidentified polar lipids; the predominant menaquinone as MK-7 and the major fatty as iso-C15:0. The genomic DNA G+C content was determined to be 38.9 mol%. The DNA–DNA hybridization values between strain YIM 101302T and S. composti T5-12T, was 53.6 ± 5.8 %. These results indicates that strain YIM 101302T represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium rhinocerotis sp. nov. is proposed. The type strain is YIM 101302T (=CCTCC AB 2013218T = KCTC 42533T).
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This research was supported by the National Natural Science Foundation of China (No. 31270001 and No. 31460005) and Yunnan Provincial Society Development Project (2014BC006). Thank for the help of teachers Ling-ling Yang and Chun-hua Yang.
Gui-Ding Li and Xiu Chen contributed equally to this work.
The GenBank accession number for the 16S rRNA gene sequence of strain YIM 101302T is KJ190943.
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Li, GD., Chen, X., Li, QY. et al. Sphingobacterium rhinocerotis sp. nov., isolated from the faeces of Rhinoceros unicornis . Antonie van Leeuwenhoek 108, 1099–1105 (2015). https://doi.org/10.1007/s10482-015-0563-7
- Sphingobacterium rhinocerotis sp. nov.
- Rhinoceros unicornis