Sinomonas halotolerans sp. nov., an actinobacterium isolated from a soil sample

Abstract

A novel actinobacterial strain, designated CFH S0499T, was isolated from a soil sample collected from Catba island in Halong Bay, Vietnam. The cells were observed to be Gram-stain positive, aerobic, non-motile, curved rods. The strain was found to grow optimally at 28 °C and pH 7.0. Growth was found to occur at 0–7 % NaCl. Chemotaxonomically, the peptidoglycan type was determined to be of the A3α type, with glutamic acid, glycine, alanine and lysine as the major cell wall amino acids. The whole cell sugars were found to contain mannose, galactose, glucose, ribose and rhamnose. The polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, glycolipids and two unidentified phospholipids. The major fatty acids were identified as anteiso-C15:0, iso-C15:0, anteiso-C17:0 and iso-C16:0 and the predominant respiratory quinone as MK-9 (H2), with a minor amount of MK-10 (H4) and MK-8 (H2). The G+C content of the genomic DNA was determined to be 71.8 mol%. The 16S rRNA gene sequence analysis showed that strain CFH S0499T should be assigned to the genus Sinomonas and is closely related to members of the species Sinomonas atrocyanea DSM 20127T (98.3 %), Sinomonas soli CW 59T (98.28 %), Sinomonas flava CW 108T (98.26 %), Sinomonas mesophila MPLK 26T (97.5 %) and Sinomonas notoginsengisoli SYP-B 575T (95.8 %). DNA–DNA hybridizations showed low values (49.1–54.5 %) between strain CFH S0499T and its four closest neighbours. Based on phenotypic, chemotaxonomic and phylogenetic analysis, strain CFH S0499T is concluded to represent a novel species of the genus Sinomonas, for which the name Sinomonas halotolerans sp. nov. is proposed, with CFH S0499T as the type strain (=CCTCC AB2014300T = KCTC 39116T).

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Acknowledgments

The authors are grateful to Prof. Hans-Peter Klenk (DSMZ, Germany) and Prof. Yu Zhou (Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, China) for their kind providing the reference type strains. This research was supported by Natural Science Foundation of China (No. 31372545), Program for Innovative Research Team (in Science and Technology) in University of Henan Province (14IRTSTHN013), Plan for Scientific Innovation Talent of Henan Province (154100510010), Scientific Research Fund of Xinxiang Medical University (2013QN126), Research Project of Education Department of Henan Province of China (2011A180025). WJ Li was also supported by Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme (2014).

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Correspondence to Wen-Jun Li or Guo-Xing Nie.

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Qian-Qian Guo and Hong Ming have contributed equally to this work.

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Guo, QQ., Ming, H., Meng, XL. et al. Sinomonas halotolerans sp. nov., an actinobacterium isolated from a soil sample. Antonie van Leeuwenhoek 108, 887–895 (2015). https://doi.org/10.1007/s10482-015-0543-y

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Keywords

  • Sinomonas halotolerans sp. nov.
  • Family Micrococcaceae
  • Halong Bay