Oceanobacillus damuensis sp. nov. and Oceanobacillus rekensis sp. nov., isolated from saline alkali soil samples

Abstract

Two moderately halophilic strains, PT-11T and PT-20T, were isolated from saline alkali soil samples collected in Shache County, Xinjiang Province, China. Both strains are aerobic, Gram-positive, motile rods. Strain PT-11T grows at 15–40 °C and at pH 6.5–10.0, while PT-20T grows at 15–40 °C and at pH 6.5–11.0. The major cellular fatty acids in both strains include anteiso-C15:0, anteiso-C17:0 and iso-C15:0. For both strains, the polar lipids consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and several unidentified lipids. In addition, strain PT-20T also contains phosphatidylcholine. The major isoprenoid quinone for both strains is MK-7. The genomic G+C content is 36.7 % for PT-11T and 39.2 % for PT-20T. Phylogenetic analyses of 16S rRNA gene sequences indicated that these two isolates are members of the genus Oceanobacillus. DNA–DNA hybridization indicated that strains PT-11T and PT-20T should be considered two distinct species. On the basis of both phylogenetic and chemotaxonomic data analyses, therefore, we conclude that PT-11T and PT-20T represent two novel species within the genus Oceanobacillus, for which we propose the names Oceanobacillus rekensis sp. nov. and Oceanobacillus damuensis sp. nov., respectively. The type strains are PT-11T (=KCTC 33144T = DSM 26900T) and PT-20T (=KCTC 33146T = DSM 26901T).

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3

References

  1. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    CAS  PubMed  Article  Google Scholar 

  2. Amoozegar MA, Bagheri M, Makhdoumi-Kakhki A, Didari M, Schumann P, Spröer C, Sánchez-Porro C, Ventosa A (2014) Oceanobacillus limi sp. nov., a moderately halophilic bacterium from a salt lake. Int J Syst Evol Microbiol 64:1284–1289

    CAS  PubMed  Article  Google Scholar 

  3. Barrow G I, Feltham RKA (1993). Cowan and Steel’s Manual for the identification of Medical bacteria, 3rd edn. Cowan & Steels Manual for the Identification of Medical Bacteria

  4. Cappuccino JG, Sherman N (2011) Microbiology: A Laboratory Manual. Pearson/Benjamin Cummings, San Francisco

    Google Scholar 

  5. Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229

    Article  Google Scholar 

  6. Heyrman J, Logan NA, Busse HJ, Balcaen A, Lebbe L, Rodriguez-Diaz M, Swings J, De Vos P (2003) Virgibacillus carmonensis sp. nov., Virgibacillus necropolis sp. nov. and Virgibacillus picturae sp. nov., three novel species isolated from deteriorated mural paintings, transfer of the species of the genus Salibacillus to Virgibacillus, as Virgibacillus marismortui comb. nov. and Virgibacillus salexigens comb. nov., and emended description of the genus Virgibacillus. Int J Syst Evol Microbiol 53:501–511

    CAS  PubMed  Article  Google Scholar 

  7. Hiraishi A, Hoshino Y (1984) Distribution of rhodoquinone in Rhodospirillaceae and its taxonomic implications. J Gen Appl Microbiol 30:435–448

    CAS  Article  Google Scholar 

  8. Hiraishi A, Hoshino Y, Kitamura H (1984) Isoprenoid quinone composition in the classification of Rhodospirillaceae. J Gen Appl Microbiol 30:197–210

  9. Hirota K, Aino K, Nodasaka Y, Yumoto I (2013a) Oceanobacillus indicireducens sp. nov., a facultative alkaliphile that reduces an indigo dye. Int J Syst Evol Microbiol 63:1437–1442

    PubMed  Article  Google Scholar 

  10. Hirota K, Hanaoka Y, Nodasaka Y, Yumoto I (2013b) Oceanobacillus polygoni sp. nov., a facultatively alkaliphile isolated from indigo fermentation fluid. Int J Syst Evol Microbiol 63:3307–3312

    CAS  PubMed  Article  Google Scholar 

  11. Imhoff JF (1984) Quinones of phototrophic purple bacteria. FEMS Microbiol Lett 25:85–89

    CAS  Article  Google Scholar 

  12. Kämpfer P, Falsen E, Lodders N, Langer S, Busse HJ, Schumann P (2010) Ornithinibacillus contaminans sp. nov., an endospore-forming species. Int J Syst Evol Microbiol 60:2930–2934

    PubMed  Article  Google Scholar 

  13. Kates M, Work TS, Work E (1972). Techniques of lipidology: isolation, analysis and identification of lipids. Lab Tech Biochem Mol Biol 267

  14. Kim YG, Choi DH, Hyun S, Cho BC (2007) Oceanobacillus profundus sp. nov., isolated from a deep-sea sediment core. Int J Syst Evol Microbiol 57:409–413

    CAS  PubMed  Article  Google Scholar 

  15. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Lee JH, Yi H, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721

    CAS  PubMed  Article  Google Scholar 

  16. Kim W, Chatuphon S, Kim JH, Sukhoom A (2015) Oceanobacillus arenosus sp.nov., a moderate halophilic bacterium isolated from marine sand. Int J Syst Evol Microbiol 63:3307–3312

    Google Scholar 

  17. Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    CAS  PubMed  Article  Google Scholar 

  18. Kimura M (1984) The neutral theory of molecular evolution. Cambridge University Press, New York

    Google Scholar 

  19. Lee JS, Lim JM, Lee KC, Lee JC, Park YH, Kim CJ (2006) Virgibacillus koreensis sp. nov., a novel bacterium from a salt field, and transfer of Virgibacillus picturae to the genus Oceanobacillus as Oceanobacillus picturae comb. nov. with emended descriptions. Int J Syst Evol Microbiol 56:251–257

    CAS  PubMed  Article  Google Scholar 

  20. Lee SY, Oh TK, Kim W, Yoon JH (2010) Oceanobacillus locisalsi sp. nov., isolated from a marine solar saltern. Int J Syst Evol Microbiol 60:2758–2762

    CAS  PubMed  Article  Google Scholar 

  21. Lee DC, Kang H, Weerawongwiwat V, Kim B, Choi YW, Kim W (2013) Oceanobacillus chungangensis sp. nov., isolated from a sand dune. Int J Syst Evol Microbiol 63:3666–3671

    CAS  PubMed  Article  Google Scholar 

  22. Li WJ, Xu P, Schumann P, Zhang YQ, Pukall R, Xu LH, Stackebrandt E, Jiang CL (2007) Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57:1424–1428

    PubMed  Article  Google Scholar 

  23. Lu J, Nogi Y, Takami H (2001) Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge. FEMS Microbiol Lett 205:291–297

    CAS  PubMed  Article  Google Scholar 

  24. Mayr R, Busse HJ, Worliczek HL, Ehling-Schulz M, Scherer S (2006) Ornithinibacillus gen. nov., with the species Ornithinibacillus bavariensis sp. nov. and Ornithinibacillus californiensis sp. nov. Int J Syst Evol Microbiol 56:1383–1389

  25. Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167

    CAS  Article  Google Scholar 

  26. Murray RGE, Doetsch RN, Robinow CF (1994) Determinative and cytological light microscopy. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, pp 21–41

    Google Scholar 

  27. Nam JH, Bae W, Lee DH (2008) Oceanobacillus caeni sp. nov., isolated from a Bacillus-dominated wastewater treatment system in Korea. Int J Syst Evol Microbiol 58:1109–1113

    CAS  PubMed  Article  Google Scholar 

  28. Namwong S, Tanasupawat S, Lee KC, Lee JS (2009) Oceanobacillus kapialis sp. nov., from fermented shrimp paste in Thailand. Int J Syst Evol Microbiol 59:2254–2259

    CAS  PubMed  Article  Google Scholar 

  29. Raats D, Halpern M (2007) Oceanobacillus chironomi sp. nov., a halotolerant and facultatively alkaliphilic species isolated from a chironomid egg mass. Int J Syst Evol Microbiol 57:255–259

    CAS  PubMed  Article  Google Scholar 

  30. Raj PS, Ramaprasad EVV, Vaseef S, Sasikala C, Ramana CV (2013) Rhodobacter viridis sp. nov., a phototrophic bacterium isolated from mud of a stream. Int J Syst Evol Microbiol 63:181–186

    CAS  PubMed  Article  Google Scholar 

  31. Rosselló-Mora R (2006) DNA-DNA reassociation methods applied to microbial taxonomy and their critical evaluation. Molecular identification, systematics, and population structure of prokaryotes. Springer Berlin, Heidelberg, pp 23–50

    Google Scholar 

  32. Smibert RM, Krieg NR (1994) Phenotypic characteristics. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Manual of Methods for General and Molecular Bacteriology. American Society for Microbiology, Washington, DC, pp 607–654

    Google Scholar 

  33. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    CAS  PubMed Central  PubMed  Article  Google Scholar 

  34. Tominaga T, An S-Y, Oyaizu H, Yokota A (2009) Oceanobacillus soja sp. nov. isolated from soy sauce production equipment in Japan. J Gen Appl Microbiol 55:225–232

    CAS  PubMed  Article  Google Scholar 

  35. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Murray RGE et al (1987) International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464

    Article  Google Scholar 

  36. Whon TW, Jung MJ, Roh SW, Nam YD, Park EJ, Shin KS, Bea JW (2010) Oceanobacillus kimchii sp. nov. isolated from a traditional Korean fermented food. J Microbiol 48(6):862–866

    CAS  PubMed  Article  Google Scholar 

  37. Wu M, Yang GQ, Yu Z, Zhuang L, Jin YQ, Zhou SG (2014) Oceanobacillus luteolus sp nov., isolated from soil. Int J Syst Evol Microbiol 64:1495–1500

    CAS  PubMed  Article  Google Scholar 

  38. Yang JY, Huo YY, Xu XW, Meng FX, Wu M, Wang CS (2010) Oceanobacillus neutriphilus sp. nov., isolated from activated sludge in a bioreactor. Int J Syst Evol Microbiol 60:2409–2414

    CAS  PubMed  Article  Google Scholar 

  39. Yu C, Yu S, Zhang Z, Li Z, Zhang XH (2014) Oceanobacillus pacificus sp. nov., isolated from a deep-sea sediment. Int J Syst Evol Microbiol 64:1278–1283

    CAS  PubMed  Article  Google Scholar 

  40. Yumoto I, Yamazaki K, Hishinuma M, Nodasaka Y, Suemori A, Nakajima K, Inoue N, Kawasaki K (2001) Pseudomonas alcaliphila sp. Nov., a novel facultatively psychrophilic alkaliphile isolated from seawater. Int J Syst Evol Microbiol 51:349–355

    CAS  PubMed  Google Scholar 

  41. Yumoto I, Hirota K, Nodasaka Y, Nakajima K (2005) Oceanobacillus oncorhynchi sp. nov., a halotolerant obligate alkaliphile isolated from the skin of a rainbow trout (Oncorhynchus mykiss), and emended description of the genus Oceanobacillus. Int J Syst Evol Microbiol 55:1521–1524

    CAS  PubMed  Article  Google Scholar 

Download references

Acknowledgments

We are grateful to Shu-Kun Tang for his help with some of the experiments. This work is supported by the Fundamental Research Funds for the Central University and Sichuan Science and Technology Project No. 2014JY0199.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Yongqiang Tian.

Electronic supplementary material

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Long, X., Ye, R., Zhang, S. et al. Oceanobacillus damuensis sp. nov. and Oceanobacillus rekensis sp. nov., isolated from saline alkali soil samples. Antonie van Leeuwenhoek 108, 731–739 (2015). https://doi.org/10.1007/s10482-015-0529-9

Download citation

Keywords

  • Oceanobacillus damuensis sp. nov.
  • Oceanobacillus rekensis sp. nov.
  • Saline alkali soil
  • Taxonomy