Oceanobacillus damuensis sp. nov. and Oceanobacillus rekensis sp. nov., isolated from saline alkali soil samples

Antonie van Leeuwenhoek volume 108, pages731–739(2015)Cite this article

Abstract

Two moderately halophilic strains, PT-11T and PT-20T, were isolated from saline alkali soil samples collected in Shache County, Xinjiang Province, China. Both strains are aerobic, Gram-positive, motile rods. Strain PT-11T grows at 15–40 °C and at pH 6.5–10.0, while PT-20T grows at 15–40 °C and at pH 6.5–11.0. The major cellular fatty acids in both strains include anteiso-C15:0, anteiso-C17:0 and iso-C15:0. For both strains, the polar lipids consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and several unidentified lipids. In addition, strain PT-20T also contains phosphatidylcholine. The major isoprenoid quinone for both strains is MK-7. The genomic G+C content is 36.7 % for PT-11T and 39.2 % for PT-20T. Phylogenetic analyses of 16S rRNA gene sequences indicated that these two isolates are members of the genus Oceanobacillus. DNA–DNA hybridization indicated that strains PT-11T and PT-20T should be considered two distinct species. On the basis of both phylogenetic and chemotaxonomic data analyses, therefore, we conclude that PT-11T and PT-20T represent two novel species within the genus Oceanobacillus, for which we propose the names Oceanobacillus rekensis sp. nov. and Oceanobacillus damuensis sp. nov., respectively. The type strains are PT-11T (=KCTC 33144T = DSM 26900T) and PT-20T (=KCTC 33146T = DSM 26901T).

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Acknowledgments

We are grateful to Shu-Kun Tang for his help with some of the experiments. This work is supported by the Fundamental Research Funds for the Central University and Sichuan Science and Technology Project No. 2014JY0199.

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Affiliations

  1. Key Laboratory of Leather Chemistry and Engineering, Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu, 610065, People’s Republic of China

    Xiufeng Long, Renyuan Ye, Shuai Zhang, Bo Liu & Yongqiang Tian

  2. Department of Pharmaceutical and Biological Engineering, College of Chemical Engineering, Sichuan University, Chengdu, 610065, Sichuan, People’s Republic of China

    Renyuan Ye & Bo Liu

  3. Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, People’s Republic of China

    Yuqin Zhang

  4. Sichuan Industrial Institute of Antibiotics, Chengdu University, No.168 Zhonghe Rd, Longtan Urban Industry Concentrate Zone, 2nd Section, East 3rd Rd, Chengdu, 610052, Sichuan, China

    Zhigang Zeng

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  1. Xiufeng Long
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Correspondence to Yongqiang Tian.

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Long, X., Ye, R., Zhang, S. et al. Oceanobacillus damuensis sp. nov. and Oceanobacillus rekensis sp. nov., isolated from saline alkali soil samples. Antonie van Leeuwenhoek 108, 731–739 (2015). https://doi.org/10.1007/s10482-015-0529-9

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Keywords

  • Oceanobacillus damuensis sp. nov.
  • Oceanobacillus rekensis sp. nov.
  • Saline alkali soil
  • Taxonomy