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Bounagaea algeriensis gen. nov., sp. nov., an extremely halophilic actinobacterium isolated from a Saharan soil of Algeria

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Abstract

A novel halophilic actinobacterium strain, designated H8T, was isolated from a Saharan soil sample collected in El-Goléa, South Algeria. Strain H8T was identified as representing a new genus using a polyphasic taxonomic approach. Phylogenetic analysis revealed that strain H8T shared the highest degree of 16S rRNA gene sequence similarity with ‘Mzabimyces algeriensis’ DSM 46680T (93.0 %), Saccharopolyspora ghardaiensis DSM 45606T (91.2 %), Halopolyspora alba DSM 45976T (90.8 %) and Actinopolyspora mortivallis DSM 44261T (90.0 %). The strain was found to grow optimally at 28–35 °C, at pH 6.0–7.0, and in the presence of 15–25 % (w/v) NaCl. The substrate mycelium was observed to be well developed and fragmented in liquid medium and on solid medium. The aerial mycelium was observed to be moderately abundant and to form long chains with non-motile, smooth-surfaced and ovoid or spherical spores at maturity. The cell wall of strain H8T was found to contain meso-diaminopimelic acid. The whole-cell hydrolysates were found to mainly contain arabinose and galactose. The diagnostic phospholipid detected was phosphatidylcholine, and MK-9(H4), MK-9(H2) and MK-10(H2) were found to be the predominant menaquinones. The major cellular fatty acids were determined to be anteiso-C17:0 and iso-C15:0. The genomic DNA G+C content of strain H8T was determined to be 71.3 mol%. The genotypic and phenotypic data showed that the strain represents a novel genus and species, for which the name Bounagaea algeriensis gen. nov., sp. nov. is proposed, with the type strain H8T (=DSM 45966T = CECT 8470T).

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Acknowledgments

The authors are grateful to Gabriele Pötter (DSMZ) for growing Bounagaea algeriensis cultures and for assistance with chemotaxonomic analyses.

Conflict of interest

The authors declare that there are no conflicts of interest to declare.

Ethical statement

This article does not contain any studies with human participants and/or animals performed by any of the authors. The formal consent is not required in this study.

Funding

This work was supported by the Ministère de l’Enseignement Supérieur et de la Recherche Scientifique (MESRS) of Algeria. The funder had no role in study design, data collection and analyses, preparation of the manuscript or decision to publish.

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Authors and Affiliations

  1. Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria

    Atika Meklat, Noureddine Bouras, Salim Mokrane, Abdelghani Zitouni & Nasserdine Sabaou

  2. Département de Biologie et Physiologie Cellulaire, Faculté des Sciences de la Nature et de la Vie, Université Saâd Dahleb, Blida, Algeria

    Atika Meklat

  3. Département de Biologie, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre, Université de Ghardaïa, BP 455, Ghardaïa, 47000, Algeria

    Noureddine Bouras

  4. Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Brunswick, Germany

    Peter Schumann & Cathrin Spröer

  5. School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, UK

    Hans-Peter Klenk

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  1. Atika Meklat
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  2. Noureddine Bouras
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Correspondence to Nasserdine Sabaou.

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Meklat, A., Bouras, N., Mokrane, S. et al. Bounagaea algeriensis gen. nov., sp. nov., an extremely halophilic actinobacterium isolated from a Saharan soil of Algeria. Antonie van Leeuwenhoek 108, 473–482 (2015). https://doi.org/10.1007/s10482-015-0500-9

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