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Bacillus salitolerans sp. nov., a novel bacterium isolated from a salt mine in Xinjiang province, China

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Abstract

A novel aerobic bacterium, KC1T, was isolated from a salt mine in Kuche county, Xinjiang province, China. Cells were observed to be Gram-positive, rod-shaped, endospore-forming and motile with flagella. Strain KC1T was found to grow at 25–45 °C (optimum 37 °C), pH 6.5–9.0 (optimum 8.0) and NaCl 0–10 % (v/v) (optimum 4 %). The major fatty acids were identified as anteiso-C15:0 and anteiso-C17:0. Menaquinone-7 (MK-7) was found to be the predominant isoprenoid quinone. The cell-wall diamino acid was found to be meso-diaminopimelic acid. Polar lipid analysis revealed the presence of phosphatidylglycerol and a glycolipid. The 16S rRNA gene sequence of strain KC1T showed low similarity (<96 %) to other validly named species. The phylogenetic trees showed that strain KC1T is closely related to Bacillus azotoformans DSM 1046T and Bacillus methanolicus DSM 16454T. Both these type strains showed 95.4 % 16S rRNA gene sequence similarity to strain KC1T. The DNA G+C content of strain KC1T was determined to be 39.0 mol%. On the basis of its phenotypic, chemotaxonomic and genotypic characteristics, strain KC1T is considered to represent a novel species of the genus Bacillus, for which the name Bacillus salitolerans sp. nov. is proposed. The type strain is KC1T (=JCM 19760T = CGMCC 1.12810T).

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (No. 31170001).

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Authors and Affiliations

  1. College of Life Sciences, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Wei-Yan Zhang, Jing Hu, Xu-Fen Zhu & Min Wu

  2. College of Forestry and Biotechnology, Zhejiang Agricultural and Forestry University, Lin’an, 311300, People’s Republic of China

    Xin-Qi Zhang

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  1. Wei-Yan Zhang
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Correspondence to Min Wu.

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Zhang, WY., Hu, J., Zhang, XQ. et al. Bacillus salitolerans sp. nov., a novel bacterium isolated from a salt mine in Xinjiang province, China. Antonie van Leeuwenhoek 108, 443–451 (2015). https://doi.org/10.1007/s10482-015-0497-0

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