Abstract
A novel agarolytic, Gram-stain negative, heterotrophic, facultatively anaerobic and pale-white pigmented bacterial strain, designated Q1T, was isolated from the marine alga Porphyra yezoensis Ueda (AST58-103) collected from the coastal area of Weihai, China. The cells are motile by means of peritrichous flagella. The isolate requires NaCl for growth, while seawater is not necessary, and growth occurs optimally at about 30–33 °C, in 1–3 % (w/v) NaCl and at pH 7–7.5. Strain Q1T shows oxidase-positive and catalase-negative activities, and possesses the ability to hydrolyse starch and alginate, but not cellulose, gelatin, urea or Tween-80. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain Q1T is affiliated with the family Alteromonadaceae within the class Gammaproteobacteria. The isolate, strain Q1T, is most closely related to Catenovulum agarivorans YM01T (94.85 %), with less than 91.2 % sequence similarity to other close relatives with validly published names. The draft genome sequence of strain Q1T consists of 62 contigs (>200 bp) of 4,548,270 bp. The genomes of Q1T and YM01T have an ANI value of 70.7 %, and the POCP value between the two genomes is 64.4 %. The genomic DNA G+C content of strain Q1T is 37.9 mol% as calculated from the draft genome sequence. The main isoprenoid quinone is ubiquinone-8. The predominant cellular fatty acids are summed feature 3 (C16:1 ω7c and/or iso-C15:0 2-OH), C16:0 and C18:1 ω7c. The major polar lipids are phosphatidylethanolamine and phosphatidylglycerol. Based on data from a polyphasic chemotaxonomic, physiological and biochemical study, strain Q1T should be classified as a novel species of the genus Catenovulum, for which the name Catenovulum maritimus sp. nov. is proposed. The type strain is Q1T (=CICC 10836T=DSM 28813T).
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (31370057), the National Science and Technology Major Project of China (2013ZX10004217), Shandong Provincial Programs for Science and Technology Development of China (2011GSF12108), and the 2013 Shandong Provincial Second Group Projects on Resource Platforms for Marine Economic and Innovative Development Regions: Marine Microorganisms Preservation Platform (2150299). We thank Prof. Xiao-Hua Zhang for kindly providing the reference strain Catenovulum agarivorans YM01T.
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The GenBank accession number for the 16S rRNA gene sequence of Catenovulum maritimus Q1T is KJ659912.
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Li, DQ., Zhou, YX., Liu, T. et al. Catenovulum maritimus sp. nov., a novel agarolytic gammaproteobacterium isolated from the marine alga Porphyra yezoensis Ueda (AST58-103), and emended description of the genus Catenovulum . Antonie van Leeuwenhoek 108, 427–434 (2015). https://doi.org/10.1007/s10482-015-0495-2
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DOI: https://doi.org/10.1007/s10482-015-0495-2