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Two-component system YvqEC-dependent bacterial resistance against vancomycin in Bacillus thuringiensis

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Abstract

YvqEC is one of the two-component signal transduction systems that may respond to cell envelope stress and enable cells to adjust multiple cellular functions. It consists of a histidine kinase YvqE and a response regulator YvqC. In this study, we separately constructed a single gene mutant ΔyvqE and a double gene mutant ΔyvqEC in Bacillus thuringiensis BMB171 through a homing endonucleases I-SceI mediated markerless gene deletion method. We found that the deletion of either yvqE or yvqEC weakened the resistance of B. thuringiensis against vancomycin. We also identified nine operons that may be involved in the cellular metabolism regulated by YvqC. This study not only enriches our understanding of bacterial resistance mechanisms against vancomycin, but also helps investigate the functions of YvqEC.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant 31270105) and the National High-tech R&D Program of China (863 Program, Grant 2011AA10A205).

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Authors and Affiliations

  1. State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, People’s Republic of China

    Shumeng Zhang, Yimin Hu, Qingyun Fan, Xun Wang & Jin He

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  1. Shumeng Zhang
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  2. Yimin Hu
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  3. Qingyun Fan
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  4. Xun Wang
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  5. Jin He
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Correspondence to Jin He.

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Zhang, S., Hu, Y., Fan, Q. et al. Two-component system YvqEC-dependent bacterial resistance against vancomycin in Bacillus thuringiensis . Antonie van Leeuwenhoek 108, 365–376 (2015). https://doi.org/10.1007/s10482-015-0489-0

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