Lampropedia puyangensis sp. nov., isolated from symptomatic bark of Populus × euramericana canker and emended description of Lampropedia hyalina (Ehrenberg 1832) Lee et al. 2004

Abstract

A Gram-stain negative, Neisser-stain negative, aerobic, non-motile, non-spore-forming, slimy, glossy bacterial strain with single or clustered coccoid cells and white colony colour, designated as 2-binT, was isolated from cankered bark tissue of Populus × euramericana. The strain was found to grow at 15–40 °C and pH 5–10, with an optimum of 30 °C and pH 8.0. The strain was found to be negative with respect to catalase and positive for oxidase activity, nitrate reduction and Voges–Proskauer reaction. Analysis of 16S rRNA gene sequence data indicated that the isolate belongs to the genus Lampropedia, having sequence similarity of 96.24 % with Lampropedia hyalina ATCC11041T. DNA–DNA relatedness of strain 2-binT with L. hyalina JCM 21380T was 26.7 ± 4.6 %. The DNA G+C content of strain 2-binT was determined to be 57 % and the major cellular fatty acids were identified as C16:0, C16:1 ω7c/C16:1 ω6c and C18:1 ω7c. The polar lipid profile of strain 2-binT was found to contain diphosphatidylglycerol, a glycolipid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, a phospholipid, phosphatidylmonomethylethanolamine and three unidentified lipids (L1, L2, L3). Based on molecular data and physiological and biochemical characteristics, strain 2-binT is considered to represent a novel species in the genus Lampropedia, for which the name Lampropedia puyangensis sp. nov. is proposed. The type strain is 2-binT (= CFCC 10925T = KCTC 32235T).

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Acknowledgments

The research was supported by the Special Fund of the Chinese Central Government for Basic Scientific Research Operations in Commonweal Research Institutes (CAFRIFEEP201102-4) and National Infrastructure of Microbial Resources (NIMR-2013-7). We also thank Professor Everett M. Hansen for his help with the written English language.

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Correspondence to Chun-gen Piao.

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Yong Li and Tao Wang have contributed equally to this work.

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Li, Y., Wang, T., Piao, Cg. et al. Lampropedia puyangensis sp. nov., isolated from symptomatic bark of Populus × euramericana canker and emended description of Lampropedia hyalina (Ehrenberg 1832) Lee et al. 2004. Antonie van Leeuwenhoek 108, 321–328 (2015). https://doi.org/10.1007/s10482-015-0483-6

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Keywords

  • Lampropedia puyangensis
  • Polyphasic taxonomy
  • DNA–DNA hybridization
  • Chemotaxonomic analysis