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Mesorhizobium soli sp. nov., a novel species isolated from the rhizosphere of Robinia pseudoacacia L. in South Korea by using a modified culture method

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Abstract

Strain NHI-8T was isolated from a forest soil sample, collected in South Korea, by using a modified culture method. Comparative analysis of its nearly full-length 16S rRNA gene sequence showed that strain NHI-8T belongs to the genus Mesorhizobium and to be closely related to Mesorhizobium chacoense PR5T (97.32 %). The levels of DNA–DNA relatedness between strain NHI-8T and reference type strains of the genus Mesorhizobium were 32.28–53.65 %. SDS-PAGE of total soluble proteins and the sequences of the housekeeping genes recA, glnII, and atpD were also used to support the clade grouping in rhizobia. The new strain contained summed feature 8 (57.0 %), cyclo-C19:0ω8c (17.3 %), and C18:0 (11.0 %) as the major fatty acids, as in genus Mesorhizobium. The strain contained cardiolipin, phosphatidylglycerol, ornithine-containing lipid, phosphatidylethanolamine, phosphatidyl-N-dimethylethanolamine, and phosphatidylcholine. Morphological and physiological analyses were performed to compare the characteristics of our strain with those of the reference type strains. Based on the results, strain NHI-8T was determined to represent a novel member of the genus Mesorhizobium, and the name Mesorhizobium soli is proposed. The type strain is NHI-8T (=KEMB 9005-153T = KACC 17916T = JCM 19897T).

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Acknowledgments

This study was supported by the Bio-industry Technology Development Program (312027-3), Minstry of Agriculture, Food and Rural Affairs, and by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science, and Technology (2011-0010144).

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Authors and Affiliations

  1. Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do, 443-760, Republic of Korea

    Tuan Manh Nguyen, Van Hong Thi Pham & Jaisoo Kim

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  1. Tuan Manh Nguyen
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  2. Van Hong Thi Pham
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  3. Jaisoo Kim
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Correspondence to Jaisoo Kim.

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The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, recA, atpD, and glnII genes are KC484966, KM188061, KM188059, and KM188060, respectively.

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Nguyen, T.M., Pham, V.H.T. & Kim, J. Mesorhizobium soli sp. nov., a novel species isolated from the rhizosphere of Robinia pseudoacacia L. in South Korea by using a modified culture method. Antonie van Leeuwenhoek 108, 301–310 (2015). https://doi.org/10.1007/s10482-015-0481-8

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