Skip to main content
SpringerLink
Log in

Marinactinospora endophytica sp. nov., isolated from a medicinal plant

  • Original Paper
  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

A novel endophytic actinobacterium, designated strain YIM 690053T, was isolated from healthy stems of Salsola ferganica Drob, and was characterized using a polyphasic taxonomic approach. The strain formed well-branched substrate mycelia with no fragmentation, and abundant aerial mycelia that differentiated into long spores. The strain was found to grow at 10–55 °C, pH 6.0–10.0 and in the presence of 0–7 % (w/v) NaCl. The genomic DNA G+C content was determined to be 70.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 690053T belongs to the genus Marinactinospora, and it shared the highest 16S rRNA gene sequence similarity with Marinactinospora thermotolerans SCSIO 00652T (98.96 %). However, the DNA–DNA relatedness values between the two strains was 37.85 ± 2.77 %. Based on the differences in the molecular and biochemical characteristics from its closest relative, strain YIM 690053T is concluded to represent a novel species of the genus Marinactinospora, for which the name Marinactinospora endophytica sp. nov. is proposed. The type strain is YIM 690053T(=KCTC 29664T = DSM 46799T).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Buck JD (1982) Non-staining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44:992–993

    PubMed Central  CAS  PubMed  Google Scholar 

  • Chen Q, Ji C, Song Y, Huang H, Ma J, Tian X, Ju J (2013) Discovery of McbB, an enzyme catalyzing the β-carboline skeleton construction in the marinacarboline biosynthetic pathway. Angew Chem Int Ed Engl 52:9980–9984

    Article  CAS  PubMed  Google Scholar 

  • Collins MD, Jones D (1980) Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48:459–470

    Article  CAS  Google Scholar 

  • Collins MD, Pirouz T, Goodfellow M, Minnikin DE (1977) Distribution of menaquinones in actinomycetes and Corynebacteria. J Gen Microbiol 100:221–230

    Article  CAS  PubMed  Google Scholar 

  • Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in micro-dilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229

    Article  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Felsenstein J (1993) PHYLIP (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle

    Google Scholar 

  • Fitch WM (1971) Toward defining the course of evolution:minimum change for a specific tree topology. Syst Zool 20:406–416

    Article  Google Scholar 

  • Gonzalez C, Gutierrez C, Ramirez C (1978) Halobacterium vallismortis sp. nov., an amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 24:710–715

    Article  CAS  PubMed  Google Scholar 

  • Groth I, Rodriguez C, Schútze B, Schmitz P, Leistner E, Goodfellow M (2004) Five novel Kitasatospora species from soil: Kitasatospora arboriphila sp. nov., K. gansuensis sp. nov., K. nipponensis sp. nov., K. paranensis sp. nov. and K. terrestris sp. nov. Int J Syst Evol Microbiol 54:2121–2129

    Article  CAS  PubMed  Google Scholar 

  • Hasegawa T, Takizawa M, Tanida S (1983) A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322

    Article  CAS  Google Scholar 

  • Huang H, Yao Y, He Z, Yang T, Ma J, Tian X, Li Y, Huang C, Chen X, Li W, Zhang S, Zhang C, Ju J (2011) Antimalarial β-carboline and indolactam alkaloids from Marinactinospora thermotolerans, a deep sea isolate. J Nat Prod 74:2122–2127

    Article  CAS  PubMed  Google Scholar 

  • Kelly KL (1964) Inter-society color council—national bureau of standards color name charts illustrated with centroid colors. US Government Printing Office, Washington, DC

    Google Scholar 

  • Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon Y, Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721

    Article  CAS  PubMed  Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  CAS  PubMed  Google Scholar 

  • Li WJ, Xu P, Schumann P, Zhang YQ, Pukall R, Xu LH, Stackebrandt E, Jiang CL (2007) Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57:1424–1428

    Article  PubMed  Google Scholar 

  • Li J, Zhao GZ, Chen HH, Wang HB, Qin S, Zhu WY, Xu LH, Jiang CL, Li WJ (2008) Antitumour and antimicrobial activities of endophytic streptomycetes from pharmaceutical plants in rainforest. Lett Appl Microbiol 47:574–580

    Article  CAS  PubMed  Google Scholar 

  • Ma J, Zuo D, Song Y, Wang B, Huang H, Yao Y, Li W, Zhang S, Zhang C, Ju J (2012) Characterization of a single gene cluster responsible for methylpendolmycin and pendolmycin biosynthesis in the deep sea bacterium Marinactinospora thermotolerans. ChemBioChem 13:547–552

    Article  CAS  PubMed  Google Scholar 

  • Marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218

    Article  CAS  Google Scholar 

  • Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167

    Article  CAS  Google Scholar 

  • Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal K, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241

    Article  CAS  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  • Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340

    Article  Google Scholar 

  • Tamaoka J, Katayama-Fujimura Y, Kuraishi H (1983) Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 54:31–36

    Article  CAS  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Tang SK, Wang Y, Chen Y, Lou K, Cao LL, Xu LH, Li WJ (2009) Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella. Int J Syst Evol Microbiol 59:2025–2033

    Article  CAS  PubMed  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Tian XP, Tang SK, Dong JD, Zhang YQ, Xu LH, Zhang S, Li WJ (2009) Marinactinospora thermotolerans gen. nov., sp. nov., a marine actinomycete isolated from a sediment in the northern South China Sea. Int J Syst Evol Microbiol 59:948–952

    Article  CAS  PubMed  Google Scholar 

  • Waksman SA (1961) The actinomycetes, vol. II. Classification, identification and description of genera and species. Williams & Wilkins, Baltimore

    Google Scholar 

  • Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE, Stackebrandt E, Starr MP, Trüper HG (1987) International committee on systematic bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464

    Article  Google Scholar 

  • Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ, Chen HH, Xu LH, Jiang CL (2005) Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55:1149–1153

  • Zhou ZH, Liu ZH, Qian YD, Kim SB, Goodfellow M (1998) Saccharopolyspora spinosporotrichia sp. nov., a novel actinomycete from soil. Int J Syst Bacteriol 48:53–58

    Article  PubMed  Google Scholar 

  • Zhou X, Huang H, Chen Y, Tan J, Song Y, Zou J, Tian X, Hua Y, Ju J (2012) Marthiapeptide A, an anti-infective and cytotoxic polythiazole cyclopeptide from a 60 L scale fermentation of the deep sea-derived Marinactinospora thermotolerans SCSIO 00652. J Nat Prod 75:2251–2255

    Article  CAS  PubMed  Google Scholar 

  • Zhu Q, Li J, Ma J, Luo M, Wang B, Huang H, Tian X, Li W, Zhang S, Zhang C, Ju J (2012) Discovery and engineered overproduction of antimicrobial nucleoside antibiotic A201A from the deep-sea marine actinomycete Marinactinospora thermotolerans SCSIO 00652. Antimicrob Agents Chemother 56:110–114

    Article  PubMed Central  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was funded jointly by Chinese national program of high technology research and development (Grant no. 2012AA021705), Yunnan Provincial Natural Science Foundation (2013FA004), China tobacco Yunnan industrial Co. Lid. (Nos. 2011CP02 and 2014YL01). WNH and W-J L extend their appreciation to the Deanship of Scientific Research at King Saud University for funding the work through the research group project no. RGP-205. W-J Li was also supported by the Hundred Talents Program of Chinese Academy of Sciences and Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme (2014).

Author information

Author notes

    Authors and Affiliations

    1. Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

      Min-Jiao Liu, Thi-Nhan Khieu & Wen-Jun Li

    2. China Tobacco Yunnan Industrial Co., Ltd, Kunming, 650231, People’s Republic of China

      Rui Gao, Wei Yang & Yan-Qing Duan

    3. Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming, 650224, People’s Republic of China

      Min-Jiao Liu & Zhi Xiong

    4. Bioproducts Research Chair (BRC), College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia

      Wael N. Hozzein

    5. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi, 830011, People’s Republic of China

      Hong-Fei Wang & Wen-Jun Li

    6. State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

      Salam Nimaichand & Wen-Jun Li

    Authors
    1. Min-Jiao Liu
      View author publications

      You can also search for this author in PubMed Google Scholar

    2. Thi-Nhan Khieu
      View author publications

      You can also search for this author in PubMed Google Scholar

    3. Rui Gao
      View author publications

      You can also search for this author in PubMed Google Scholar

    4. Wael N. Hozzein
      View author publications

      You can also search for this author in PubMed Google Scholar

    5. Hong-Fei Wang
      View author publications

      You can also search for this author in PubMed Google Scholar

    6. Wei Yang
      View author publications

      You can also search for this author in PubMed Google Scholar

    7. Salam Nimaichand
      View author publications

      You can also search for this author in PubMed Google Scholar

    8. Zhi Xiong
      View author publications

      You can also search for this author in PubMed Google Scholar

    9. Yan-Qing Duan
      View author publications

      You can also search for this author in PubMed Google Scholar

    10. Wen-Jun Li
      View author publications

      You can also search for this author in PubMed Google Scholar

    Corresponding authors

    Correspondence to Yan-Qing Duan or Wen-Jun Li.

    Additional information

    Min-Jiao Liu, Thi-Nhan Khieu and Rui Gao have contributed equally to this work.

    Electronic supplementary material

    Below is the link to the electronic supplementary material.

    Supplementary material 1 (DOC 534 kb)

    Rights and permissions

    Reprints and permissions

    About this article

    Check for updates. Verify currency and authenticity via CrossMark

    Cite this article

    Liu, MJ., Khieu, TN., Gao, R. et al. Marinactinospora endophytica sp. nov., isolated from a medicinal plant. Antonie van Leeuwenhoek 107, 1577–1582 (2015). https://doi.org/10.1007/s10482-015-0451-1

    Download citation

    • Received:

    • Accepted:

    • Published:

    • Issue Date:

    • DOI: https://doi.org/10.1007/s10482-015-0451-1

    Keywords

    Search

    Navigation