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Delftia deserti sp. nov., isolated from a desert soil sample

Antonie van Leeuwenhoek volume 107, pages 1445–1450 (2015)Cite this article

Abstract

A Gram-staining negative, short rod, motile, light brownish-pigmented bacterial strain, designated YIM Y792T, was isolated from a soil sample taken from Turpan desert in Xinjiang Uyghur Autonomous Region, north-western China. Phylogenetic analysis indicated that strain YIM Y792T belongs to the genus Delftia. Strain YIM Y792T shared highest 16S rRNA gene sequence similarities with Delftia lacustris DSM 21246T (93.96 %), Delftia tsuruhatensis NBRC 16741T (93.74 %), and Delftia acidovorans NBRC 14950T (93.62 %). Growth of the strain YIM Y792T was found to occur at 20–45 °C (optimum at 30 °C), pH 6.0–9.0 (optimum at pH 7.0), and salinities of 0–3.0 % NaCl (optimum at 1.0 %). The new bacterium exhibits typical chemotaxonomic features of the genus Delftia with ubiqinone-8 (Q-8) as the predominant quinone and C16:0, Summed feature 3, Summed Feature 8 as major fatty acids (>10 %). The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, glycolipid, two unidentified phospholipids and one unidentified lipid. The G+C content of the genomic DNA of strain YIM Y792T was found to be 70.3 mol%. The DNA–DNA relatedness values between strain YIM Y792T and D. lacustris DSM 21246T, D. tsuruhatensis NBRC16741T, D. acidovorans NBRC14950T were 35.5 ± 2.0, 17.1 ± 1.8, 26.2 ± 2.0 %. Based on the phylogenetic, chemotaxonomic and phenotypic data presented here, we propose a novel species with the name Delftia desertisoli sp. nov. The type strain is YIM Y792T (=KCTC 42377T = JCM 30639T).

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Acknowledgments

The authors are grateful to Prof. Dr. Hans-Peter Klenk (DSMZ, Germany) and Dr. Tamura Tomohiko (NBRC, Japan) for their kind providing the reference type strains. This research work was supported by Chinese National Program of High Technology Research and Development (2012AA021705) and Key Project of China Ministry of Science and Technology (2013BAD16805). WNH and WJ Li extend their appreciation to the Deanship of Scientific Research at King Saud University for funding this work through the research group no RGP-205. WJ Li was also supported by the Hundred Talents Program of Chinese Academy of Sciences and Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme (2014).

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    Authors and Affiliations

    1. Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, 130118, People’s Republic of China

      Chang-Tian Li & Zheng-Fei Yan

    2. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

      Xiao Chu, Firasat Hussain, Wen-Dong Xian & Wen-Jun Li

    3. College of Life Sciences and Technology, Xinjiang University, Urumqi, 830046, People’s Republic of China

      Zulfiya Yunus & Gulsumay Abaydulla

    4. Bioproducts Research Chair (BRC), College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia

      Wael N. Hozzein

    5. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, Xinjiang, People’s Republic of China

      Wen-Jun Li

    6. State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

      Wen-Jun Li

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    Chang-Tian Li and Zheng-Fei Yan have contributed equally to this work.

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    Li, CT., Yan, ZF., Chu, X. et al. Delftia deserti sp. nov., isolated from a desert soil sample. Antonie van Leeuwenhoek 107, 1445–1450 (2015). https://doi.org/10.1007/s10482-015-0440-4

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    Keywords

    • Delftia deserti sp. nov.
    • Turpan desert
    • Xinjiang
    • Polyphasic taxonomy