Antonie van Leeuwenhoek

, Volume 107, Issue 5, pp 1135–1144

Moderate oxygen depletion as a factor favouring the filamentous growth of Sphaerotilus natans

  • Marina Seder-Colomina
  • Anne Goubet
  • Sébastien Lacroix
  • Guillaume Morin
  • Georges Ona-Nguema
  • Giovanni Esposito
  • Eric D. Van Hullebusch
  • Jean-Jacques Pernelle
Original Paper

Abstract

Sphaerotilus natans is a neutrophilic iron-related sheath-forming filamentous microorganism that presents dual morphotype: single cells and ensheathed cells forming filaments. As S. natans has been proposed as a sorbent for inorganic pollutants and it is occasionally involved in bulking episodes, elucidating factors affecting its filamentous growth is of crucial interest. The purpose of this work was to evaluate the effect of dissolved oxygen (DO) as a factor affecting S. natans filamentation from single cells. A method to quantify S. natans in its filamentous and single-cell morphotypes, based on a differential filtration procedure coupled with quantitative real-time PCR, was developed here. Scanning Electron Microscopy was used to validate the filtration step. Under actively aerated conditions (DO maintained at 7.6 ± 0.1 mg l−1), S. natans grew mainly as single cells throughout the experiment, while a depletion in DO concentration (to ~3 mg l−1) induced its filamentous growth. Indeed, when oxygen was reduced the proportion of single cells diminished from 83.3 ± 5.9  to 14.3 ± 3.4 % while the filaments increased from 16.7 ± 5.9  to 85.7 ± 3.4 %. Our results suggest that oxygen plays a key role in S. natans filamentation and contribute to better understanding of the filamentous proliferation of this bacterium. In addition, the proposed method will be helpful to evaluate other factors favouring filamentous growth.

Keywords

Filamentous bacteria Dissolved oxygen Sphaerotilus natans qPCR 

Supplementary material

10482_2015_405_MOESM1_ESM.docx (907 kb)
Supplementary material 1 (DOCX 907 kb)

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Marina Seder-Colomina
    • 1
    • 2
  • Anne Goubet
    • 3
  • Sébastien Lacroix
    • 3
    • 5
  • Guillaume Morin
    • 4
  • Georges Ona-Nguema
    • 4
  • Giovanni Esposito
    • 2
  • Eric D. Van Hullebusch
    • 1
  • Jean-Jacques Pernelle
    • 3
  1. 1.Laboratoire Géomatériaux et Environnement (LGE) (EA 4508), Institut Francilien des Sciences AppliquéesUniversité Paris-Est UPEMMarne-la-vallée, Cedex 2France
  2. 2.Department of Civil and Mechanical EngineeringUniversity of Cassino and Southern LazioCassinoItaly
  3. 3.Institut national de recherche en sciences et technologies pour l’environnement et l’agriculture (Irstea)UR HBANAntony CedexFrance
  4. 4.Institut de Minéralogie, de Physique des Matériaux, et de Cosmochimie (IMPMC)UMR 7590, CNRS, UPMC, MNHN, IRDParis, Cedex 05France
  5. 5.Veolia Environnement Recherche and Innovation - Pôle Biotechnologies et AgronomieCentre de Recherche Veolia Environnement de Maisons LaffitteMaisons - LaffitteFrance

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