Kushneria pakistanensis sp. nov., a novel moderately halophilic bacterium isolated from rhizosphere of a plant (Saccharum spontaneum) growing in salt mines of the Karak area in Pakistan
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The taxonomic position of a Gram-stain negative, moderately halophilic bacterium, designated NCCP-934T, was investigated using polyphasic taxonomic approach. The strain NCCP-934T was isolated from rhizosphere of a plant (Saccharum spontaneum, family Poaceae) growing in salt mines area in the Karak district of Khyber Pakhtunkhwa Province, Pakistan. Cells of strain NCCP-934T are rod shaped and motile. The bacterium is strictly aerobic, can grow at a temperature range of 10–40 °C (optimum at 30–33 °C) and in a pH range of 6.0–10.5 (optimum pH 7.0–9.0). The strain can tolerate 1–30 % (w/v) NaCl (optimal growth occurs in the presence of approximately 3–9 % NaCl). The phylogenetic analysis based on the 16S rRNA gene sequence, showed that strain NCCP-934T belongs to the genus Kushneria with the highest sequence similarity to K. marisflavi SW32T (98.9 %), K. indalinina CG2.1T (98.7 %), K. avicenniae MW2aT (98.4 %) and less than 97 % similarity with other related species (94.7 % with the type species of the genus, K. aurantia A10T). DNA–DNA relatedness between strain NCCP-934T and the type strains of the closely related species was lower than 18 %. The chemotaxonomic data (major respiratory quinone, Q9; predominant fatty acids, C18:1 ω7c and C16:0 followed by C12:0 3-OH and Summed features 3 (C16:1 ω7c/iso-C15:0 2-OH); major polar lipids, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanol, phosphatidylserine, phosphatidylinositol and three polar lipid of unknown structure) supported the affiliation of strain NCCP-934T within the genus Kushneria. The DNA G+C content of strain NCCP-934T was 59.2 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain NCCP-934T can be distinguished from the closely related taxa and thus represents a novel species in the genus Kushneria, for which the name Kushneria pakistanensis sp. nov. is proposed, with the type strain NCCP-934T (=LMG 28525T = KCTC 42082T = JCM 18802T).
KeywordsHalophilic bacterium Karak salt mines Kushneria pakistanensis
This work was supported partly by financial assistance from PSDP funded Project Research for Agricultural Development Project (RADP) under a sub-project (Grant No. CS-55/RADP/PARC) entitled “Establishment of Microbial Bio-Resource Laboratories: National Culture Collection of Pakistan (NCCP)” from Pakistan Agricultural Research Council (PARC), Islamabad, Pakistan and partially from Japan Society for Promotion of Science (JSPS) under fellowship program to I.A.
- Cabrera A, Aguilera M, Fuentes S, Incerti C, Russell NJ, Ramos-Cormenzana A, Monteoliva-Sanchez M (2007) Halomonas indalinina sp. nov., a moderately halophilic bacterium isolated from a solar saltern in Cabo de Gata, Almeria, southern Spain. Int J Syst Evol Microbiol 57(Pt 2):376–380. doi: 10.1099/ijs.0.64702-0 CrossRefPubMedGoogle Scholar
- Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39(3):224–229. doi: 10.1099/00207713-39-3-224 CrossRefGoogle Scholar
- Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721. doi: 10.1099/Ijs.0.038075-0 CrossRefPubMedGoogle Scholar
- Kudo T (2001) Phospholipids. In: Suzuki K, Hiraishi A, Yokota A (eds) Identification Manual of Bacteria: Molecular Genetics and Molecular Biological Methods. Springer, Tokyo, pp 135–144Google Scholar
- LPSN (2014) List of prokaryotes standing in nomenclature: genus, Kushneria. http://www.bacterio.net/kushneria.html. Accessed 8 Sept 2014
- Roohi A, Ahmed I, Iqbal M, Jamil M (2012) Preliminary isolation and characterization of halotolerant and halophilic bacteria from salt mines of Karak, Pakistan. Pak J Bot 44(SI 1):365–370Google Scholar
- Roohi A, Ahmed I, Khalid N, Iqbal M, Jamil M (2014a) Isolation and phylogenetic identification of halotolerant/halophilic bacteria from the salt mines of Karak, Pakistan. Int J Agr Biol 16(3):564–570Google Scholar
- Sánchez-Porro C, de la Haba RR, Soto-Ramírez N, Márquez MC, Montalvo-Rodríguez R, Ventosa A (2009) Description of Kushneria aurantia gen. nov., sp. nov., a novel member of the family Halomonadaceae, and a proposal for reclassification of Halomonas marisflavi as Kushneria marisflavi comb. nov., of Halomonas indalinina as Kushneria indalinina comb. nov. and of Halomonas avicenniae as Kushneria avicenniae comb. nov. Int J Syst Evol Microbiol 59(2):397–405. doi: 10.1099/ijs.0.001461-0 CrossRefPubMedGoogle Scholar
- Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101Google Scholar
- Soto-Ramirez N, Sanchez-Porro C, Rosas S, Gonzalez W, Quinones M, Ventosa A, Montalvo-Rodriguez R (2007) Halomonas avicenniae sp nov., isolated from the salty leaves of the black mangrove Avicennia germinans in Puerto Rico. Int J Syst Evol Microbiol 57:900–905. doi: 10.1099/ijs.0.64818-0 CrossRefPubMedGoogle Scholar
- Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE, Stackebrandt E, Starr MP, Truper HG (1987) Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37(4):463–464. doi: 10.1099/00207713-37-4-463 CrossRefGoogle Scholar