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Ornithinibacillus composti sp. nov., isolated from sludge compost and emended description of the genus Ornithinibacillus

Antonie van Leeuwenhoek volume 107, pages 813–819 (2015)Cite this article

Abstract

A Gram-stain positive, aerobic, motile, endospore-forming and rod-shaped bacterium, designated GSS05T, was isolated from a sludge compost sample and was characterized by means of a polyphasic taxonomic approach. Growth was observed to occur with 0–3 % (w/v) NaCl (optimum 1 %), at pH 5.5–10 (optimum pH 7.5) and at 15–50 °C (optimum 37 °C). According to the results of a phylogenetic analysis, strain GSS05T was found to belong to the genus Ornithinibacillus and to be related most closely to the type strains of Ornithinibacillus halotolerans and Ornithinibacillus contaminans (96.5 and 95.1 % 16S rRNA gene sequence similarity, respectively). The peptidoglycan amino acid type was determined to be A4β. The major respiratory quinone was identified as menaquinone-7 (MK-7). The polar lipid profile of strain GSS05T was found to contain a predominance of diphosphatidylglycerol, moderate amounts of phosphatidylglycerol and minor amounts of two unknown phospholipids and two unknown lipids. The G+C content of genomic DNA was determined to be 42.1 mol%. The dominant cellular fatty acids were identified as iso-C15:0 and anteiso-C15:0. The phenotypic, chemotaxonomic, phylogenetic and genotypic data indicated that strain GSS05T represents a novel species of the genus Ornithinibacillus, for which the name Ornithinibacillus composti sp. nov. is proposed. The type strain is GSS05T (=CCTCC AB 2013261T = KCTC 33192T).

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Acknowledgments

This work was funded by the National Natural Science Foundation of China (41201227), the China Postdoctoral Science Foundation Grant (2013M531828), and the Science and Technology Planning Program of Guangdong Province (2012B091100271).

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Authors and Affiliations

  1. Guangdong Institute of Eco-environmental and Soil Sciences, Guangzhou, 510650, People’s Republic of China

    Qin Lu, Jibing Li & Shungui Zhou

  2. Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou, 510640, People’s Republic of China

    Haoran Yuan

  3. College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, People’s Republic of China

    Jibing Li & Yong Zhao

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  1. Qin Lu
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  2. Haoran Yuan
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  3. Jibing Li
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Correspondence to Shungui Zhou.

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Lu, Q., Yuan, H., Li, J. et al. Ornithinibacillus composti sp. nov., isolated from sludge compost and emended description of the genus Ornithinibacillus . Antonie van Leeuwenhoek 107, 813–819 (2015). https://doi.org/10.1007/s10482-014-0374-2

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Keywords

  • Ornithinibacillus composti sp. nov.
  • Polyphasic taxonomy
  • Compost
  • A4β type peptidoglycan