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Pedobacter bambusae sp. nov., isolated from soil of a bamboo plantation

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An Erratum to this article was published on 23 January 2015

Abstract

An ivory white, rod-shaped, non-motile, non-spore forming, Gram-staining-negative, and aerobic bacterium designated THG-G118T, was isolated from a soil sample collected in a bamboo plantation in Seoul, Republic of Korea. The 16S rRNA gene sequence analysis revealed a clear affiliation of strain THG-G118T with the genus Pedobacter. Strain THG-G118T was found to show the closest phylogenetic relationship with Pedobacter metabolipauper WB2.3-71T with 16S rRNA gene sequence similarity of 97.3 %. The major cellular fatty acids of strain THG-G118T were identified as C16:0, C18:0, iso-C15:0 and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), and the predominant menaquinone as menaquinone MK-7. The major polar lipids were found to be phosphatidylethanolamine (PE), unidentified phospholipid and unidentified aminophospholipids. Strain THG-G118T was found to contain ceramide phosphorylethanolamine 2 (CerPE-2) and dihydrosphingosine as the major ceramide. The G+C content of the genomic DNA was found to be 41.7 mol%. DNA–DNA hybridization experiments revealed a low level of DNA–DNA relatedness (<42.5 %) between strain THG-G118T and its closest relatives. The phenotypic, chemotaxonomic and phylogenetic data showed that strain THG-G118T could be clearly distinguished from the members of the genus Pedobacter. Based on the results of a polyphasic study, a new species, Pedobacter bambusae sp. nov., is proposed. The type strain is THG-G118T (=KACC 17544T = JCM 19364T).

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Acknowledgments

This work was conducted under the industrial infrastructure program (No. N0000888) for fundamental technologies which is funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea).

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Authors and Affiliations

  1. Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University, Yongin, Gyeonggi, 446-701, Republic of Korea

    Kyung Hwa Won & MooChang Kook

  2. Department of Marine Biotechnology, Anyang University, Incheon, 417-833, Republic of Korea

    Tae-Hoo Yi

Authors
  1. Kyung Hwa Won
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  2. MooChang Kook
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  3. Tae-Hoo Yi
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Corresponding author

Correspondence to Tae-Hoo Yi.

Additional information

The NCBI GenBank accession number for the 16S rRNA gene sequence of strain THG-G118T is KF150694.

Kyung Hwa Won and Moochang Kook authors equally contributed to this work.

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Won, K.H., Kook, M. & Yi, TH. Pedobacter bambusae sp. nov., isolated from soil of a bamboo plantation. Antonie van Leeuwenhoek 107, 565–573 (2015). https://doi.org/10.1007/s10482-014-0352-8

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