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Chryseobacterium rhizoplanae sp. nov., isolated from the rhizoplane environment

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Abstract

A slightly yellow pigmented strain (JM-534T) isolated from the rhizoplane of a field-grown Zea mays plant was investigated using a polyphasic approach for its taxonomic allocation. Cells of the isolate were observed to be rod-shaped and to stain Gram-negative. Comparative 16S rRNA gene sequence analysis showed that the isolate had the highest sequence similarities to Chryseobacterium lactis (98.9 %), Chryseobacterium joostei and Chryseobacterium indologenes (both 98.7 %), and Chryseobacterium viscerum (98.6 %). Sequence similarities to all other Chryseobacterium species were 98.5 % or below. The fatty acid analysis of the strain resulted in a Chryseobacterium typical pattern consisting mainly of the fatty acids C15:0 iso, C15:0 iso 2-OH, C17:1 iso ω9c, and C17:0 iso 3-OH. DNA–DNA hybridizations with the type strains of C. lactis, C. joostei, C. viscerum and C. indologenes resulted in values below 70 %. Genomic fingerprinting showed that the isolate was very different to the type strains of these species. Differentiating biochemical and chemotaxonomic properties showed that the isolate JM-534T represents a novel species, for which the name Chryseobacterium rhizoplanae sp. nov. (type strain JM-534T = LMG 28481T = CCM 8544T = CIP 110828T) is proposed.

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Acknowledgments

We thank Gundula Will, Maria Sowinsky, Jan Rodrigues-Fonseca and Anna Baum for excellent technical assistance.

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Authors and Affiliations

  1. Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26–32, 35392, Giessen, Germany

    Peter Kämpfer & Stefanie P. Glaeser

  2. Department of Entomology and Plant Pathology, Auburn University, Auburn, Alabama, USA

    John A. McInroy

Authors
  1. Peter Kämpfer
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  2. John A. McInroy
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  3. Stefanie P. Glaeser
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Correspondence to Peter Kämpfer.

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Kämpfer, P., McInroy, J.A. & Glaeser, S.P. Chryseobacterium rhizoplanae sp. nov., isolated from the rhizoplane environment. Antonie van Leeuwenhoek 107, 533–538 (2015). https://doi.org/10.1007/s10482-014-0349-3

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