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Streptomyces alkalithermotolerans sp. nov., a novel alkaliphilic and thermotolerant actinomycete isolated from a soda lake

Abstract

An alkaliphilic actinomycete, strain AC3T, was isolated from Lonar soda lake, in India. Based on 16S rRNA gene sequence analysis it was identified that the strain belongs to the class Actinobacteria and was most closely related to Streptomyces sodiiphilus JCM 13581T (96.4 % sequence similarity), Streptomyces leeuwenhoekii DSM 42122T (96.1 %), Streptomyces albus NRRL B-2365T (96.1 %), Streptomyces panacagri Gsoil 519T (96.0 %), Streptomyces fimbriatus NBRC 15411T (95.9 %) and other members of the genus Streptomyces (<95.9 %).The strain formed a well separated clade in the genus Streptomyces based on the 16S rRNA gene analysis of all of the validly named Streptomyces species. Strain AC3T was observed to produce light-cream substrate and white aerial mycelia on most tested media. The optimum pH for growth was determined to be 9.5–10.0 with no growth at pH 7.0. The DNA G+C content of strain AC3T was determined to be 71.2 mol %. The results of the polyphasic analysis allowed a clear differentiation of strain AC3T from all other members of the genus Streptomyces. Strain AC3T is thus considered to represent a novel member of the genus Streptomyces, for which the name Streptomyces alkalithermotolerans sp. nov. is proposed. The type strain is AC3T (=KCTC 29497T = JCM 30167T).

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Acknowledgments

The authors thank Dr. A.C. Parte for the etymology and Latin description of the species epithet. SVR and MT thank Department of Science & Technology, Government of India, for providing FTSYS project grants (SB/FT/LS-115/2012-13 and SB/FT/LS-320/2012-13, respectively). FM thanks Department of Science & Technology, Government of India, for providing PA fellowship.

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Correspondence to Vishnuvardhan Reddy Sultanpuram.

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10482_2014_332_MOESM1_ESM.docx

Supplementary material 1 (DOCX 67 kb) Supplementary Fig. 1 Whole cell amino acids extract TLC of strain AC3T and Streptomyces sodiiphilus JCM 13581T. This was developed in methanol:pyridine:water:12 N HCl (32:4:7:1 v/v)

10482_2014_332_MOESM2_ESM.docx

Supplementary material 2 (DOCX 74 kb) Supplementary Fig. 2 Whole cell sugars extract TLC of strain AC3T and Streptomyces sodiiphilus JCM 13581T. This was developed in butanol:pyridine:water (50:30:20 v/v)

10482_2014_332_MOESM3_ESM.docx

Supplementary material 3 (DOCX 363 kb) Supplementary Fig. 3. Two-dimensional thin-layer chromatogram of polar lipid extracts of AC3T and Streptomyces sodiiphilus JCM 13581T. The first direction was developed in CHCl3:CH3OH:H2O (75:32:4 by volume) and the second in CHCl3:CH3OH:CH3COOH:H2O (86:16:15:4 by volume). PG, phosphatidylglycerol; GL-glycolipid; PE, phosphatidylethanolamine; PL1-3, phospholipids

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Sultanpuram, V.R., Mothe, T. & Mohammed, F. Streptomyces alkalithermotolerans sp. nov., a novel alkaliphilic and thermotolerant actinomycete isolated from a soda lake. Antonie van Leeuwenhoek 107, 337–344 (2015). https://doi.org/10.1007/s10482-014-0332-z

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Keywords

  • Streptomyces
  • Streptomyces alkalithermotolerans sp. nov.
  • Lonar soda lake
  • Polyphasic taxonomy