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Rhizobium pakistanensis sp. nov., isolated from groundnut (Arachis hypogaea) nodules grown in rainfed Pothwar, Pakistan

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Abstract

A Gram-negative, white, non-motile, rod shaped bacterial strain BN-19T was isolated from a root nodule of groundnut (Arachis hypogaea) in Pakistan. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain BN-19T formed a subclade in the genus Rhizobium together with Rhizobium alkalisoli CCBAU 01393T, Rhizobium vignae CCBAU 05176T, Rhizobium huautlense SO2T and Rhizobium tarimense PL-41T with sequence similarities of 97.5, 97.3, 97.2 and 97.1 % respectively. Sequence analysis of housekeeping genes atpD, glnII and recA (with sequence similarities of ≤92 %) confirmed the unique position of BN-19T in the genus Rhizobium. DNA–DNA relatedness between the strain BN-19T and R. alkalisoli CCBAU 01393T, R. vignae CCBAU 05176T, R. huautlense SO2T and R. tarimense PL-41T were 20.6, 22.5, 15.9 and 20.5 % respectively, further confirming that BN-19T represents a novel species in the genus Rhizobium. The DNA G + C content was 60.1 mol%. The dominant fatty acids of strain BN-19T were C19:0 cyclo ω8c, summed feature 2 (C14:0 3OH and/or C16:1 iso I) and summed feature 8 (C18:1 ω7c). Some phenotypic features also differentiate the strain BN-19T from the related species. On the basis of these results, strain BN-19T is considered to represent a novel species in the genus Rhizobium, for which the name Rhizobium pakistanensis sp. nov. is proposed. The type strain is BN-19T (=LMG 27895T = CCBAU 101086T).

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Acknowledgments

This research was supported/financed by Higher Education Commission (HEC) of Pakistan under HEC Indigenous PhD Fellowship and International Research Support Initiative Programs (IRSIP) at Rhizobia Research Center, State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agriculture University, Beijing, China.

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Authors and Affiliations

  1. Department of Soil Science & Soil Water Conservation, PMAS-Arid Agriculture University, Rawalpindi, Pakistan

    Rabia Khalid, Safdar Ali, Ummay Amara & Rifat Hayat

  2. State Key Laboratory for Agro-Biotechnology and Ministry of Agriculture Key Lab of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China

    Rabia Khalid, Yu Jing Zhang, Xin Hua Sui & Wen Xin Chen

  3. Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Xiao Xia Zhang

Authors
  1. Rabia Khalid
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  2. Yu Jing Zhang
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  3. Safdar Ali
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  4. Xin Hua Sui
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  5. Xiao Xia Zhang
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  6. Ummay Amara
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  7. Wen Xin Chen
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  8. Rifat Hayat
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Corresponding authors

Correspondence to Rabia Khalid or Rifat Hayat.

Additional information

The GenBank/EMBL/DDBJ sequence accession numbers of strain BN-19T (=LMG 27895T = CCBAU 101086T) for the partial 16S rRNA, atpD, glnII and recA gene are AB854065, AB856324, AB856325 and AB855792, respectively.

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Khalid, R., Zhang, Y.J., Ali, S. et al. Rhizobium pakistanensis sp. nov., isolated from groundnut (Arachis hypogaea) nodules grown in rainfed Pothwar, Pakistan. Antonie van Leeuwenhoek 107, 281–290 (2015). https://doi.org/10.1007/s10482-014-0326-x

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