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Streptomyces klenkii sp. nov., isolated from deep marine sediment

Antonie van Leeuwenhoek volume 107, pages 273–279 (2015)Cite this article

Abstract

A novel actinomycete strain, designated S2704T, was isolated from a deep sediment sample, collected from the southern Black Sea coast, Turkey and characterized using a polyphasic approach. Comparison of 16S rRNA gene sequences showed that strain S2704T is a member of the genus Streptomyces, exhibiting highest similarity with Streptomyces specialis GW 41-1564T (97.1 %). Strain S2704T could be distinguished from all other Streptomyces species with validly published names by sequence similarity values less than 97.0 %. The isolate was found to contain LL-diaminopimelic acid as the diagnostic diamino acid. Whole cell hydrolysates were found to contain glucose, mannose and ribose. The polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, glycophospholipid, a phospholipid and two unknown glycolipids. The predominant menaquinones were identified as MK-9(H8) and MK-10(H6). The major fatty acids were found to be anteiso-C17:0, iso-C16:0 and anteiso-C15:0. The G+C content of the DNA was determined to be 72.2 mol%. On the basis of its phenotypic and molecular properties, strain S2704T is considered to be a novel species of the genus Streptomyces, for which the name Streptomyces klenkii sp. nov. is proposed. The type strain is S2704T (=DSM 42104T = KCTC 29202T).

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Acknowledgments

This research was supported by Ondokuz Mayis University (OMU), project no. PYO. FEN. 1901.12.014.

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  1. Aysel Veyisoglu

    Present address: Department of Molecular Biology and Genetics, Faculty of Sciences, Canik Basari University, 55080, Samsun, Turkey

Authors and Affiliations

  1. Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, Kurupelit, 55139, Samsun, Turkey

    Aysel Veyisoglu & Nevzat Sahin

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  1. Aysel Veyisoglu
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  2. Nevzat Sahin
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Correspondence to Nevzat Sahin.

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Veyisoglu, A., Sahin, N. Streptomyces klenkii sp. nov., isolated from deep marine sediment. Antonie van Leeuwenhoek 107, 273–279 (2015). https://doi.org/10.1007/s10482-014-0325-y

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Keywords

  • Streptomycetaceae
  • Streptomyces klenkii
  • Polyphasic taxonomy
  • Southern Black Sea coast