Skip to main content

Myroides injenensis sp. nov., a new member isolated from human urine


A Gram-negative, yellow-pigmented, rod-shaped bacteria, designated M09-0166Tand M09-1053 were isolated from human urine samples. 16S rRNA gene sequence analysis revealed that the isolates belong to the Myroides cluster and were closely related to Myroides phaeus DSM 23313T (96.3 %), Myroides odoratimimus KCTC 23053T (96.1 %), Myroides profundi KCTC 23066T (96.0 %), Myroides odoratus KCTC 23054T (95.4 %) and Myroides pelagicus KCTC 12661T (95.2 %). The major mena quinone was identified as MK-6. The major polar lipids were identified as phosphatidylethanolamine, amino lipids, and several unknown lipids, and the major fatty acids as iso-C15:0 and iso-C17:0 3-OH. Phenotypic and chemotaxonomic data supported the affiliation of the isolates with the genus Myroides and clearly indicated that two isolates represent novel species, for which the name Myroides injenensis sp. nov. (type strain, M09-0166T = KCTC 23367T = JCM 17451T) is proposed.

This is a preview of subscription content, access via your institution.

Fig. 1


  • Atlas RM (2010) Handbook of microbiological media, 4th edn. Taylor & Francis, Boca Raton

    Book  Google Scholar 

  • Chang YH, Jung MY, Park IS, Oh HM (2008) Sporolactobacillus vineae sp. nov., a spore-forming lactic acid bacterium isolated from vineyard soil. Int J Syst Evol Microbiol 58:2316–2320

    CAS  PubMed  Article  Google Scholar 

  • Cho SH, Chae SH, Im WT, Kim SB (2011) Myroides marinus sp. nov., a member of the family Flavobacteriaceae, isolated from seawater. Int J Syst Evol Microbiol 61:938–941

    CAS  PubMed  Article  Google Scholar 

  • Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17(6):368–376

    CAS  PubMed  Article  Google Scholar 

  • Felsenstein J (1985) Confidence limit on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Felsenstein J (1993) Phylogeny Inference Package (PHYLIP), version 3.5. distributed by the author. Department of Genome Sciences, University of Washington, Seattles

  • Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416

    Article  Google Scholar 

  • Jeon YS, Chung H, Park S, Hur I, Lee JH, Chun J (2005) jPHYDIT: a JAVA-based integrated environment for molecular phylogeny of ribosomal RNA sequences. Bioinformatics 21(14):3171–3173

    CAS  PubMed  Article  Google Scholar 

  • Kimura M (1983) The neutral theory of molecular evolution. Cambridge University, Cambridge

    Book  Google Scholar 

  • Komagata K, Suzuki K (1987) Lipid and cell-wall analysis in bacterial systematic. In: Colwell RR, Grigorova R (eds) Method in microbiology, vol 19. Academic Press, London, pp 161–207

    Google Scholar 

  • Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167

    CAS  Article  Google Scholar 

  • MIDI (1999) Sherlock microbial identification system, operating manual version 3.0. MIDI, Newark

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  Google Scholar 

  • Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, pp 607–654

    Google Scholar 

  • Stackebrandt E, Ebers J (2006) Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155

    Google Scholar 

  • Tindall BJ (1990) A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13(2):128–130

    CAS  Article  Google Scholar 

  • Tittsler RP, Sandholzer LA (1936) The use of semi-solid agar for the detection of bacterial motility. J Bacteriol 31(6):575–580

    CAS  PubMed Central  PubMed  Google Scholar 

  • Tomova A, Tomova I, Vasileva-Tonkova E, Lazarkevich I, Stoilova-Disheva M, Lyutskanova D, Kambourova M (2013) Myroides guanonis sp. nov., isolated from prehistoric paintings. Int J Syst Evol Microbiol 63(Pt 11):4266–4270

    Article  Google Scholar 

  • Vancanneyt M, Segers P, Torck U, Hoste B, Bernardet J-F, Vandamme P, Kersters K (1996) Reclassification of Flavobacterium odoratum (Stutzer 1929) strains to a new genus, Myroides, as Myroides odoratus comb. nov. and Myroides odoratimimus sp. nov. Int J Syst Bacteriol 46(4):926–932

    Article  Google Scholar 

  • Woese CR, Magrum LJ, Gupta R, Siegel RB, Stahl DA, Kop J, Crawford N, Brosius J, Gutell R, Hogan JJ, Noller HF (1980) Secondary structure model for bacterial 16S ribosomal RNA: phylogenetic, enzymatic and chemical evidence. Nucleic Acids Res 8(10):2275–2293

    CAS  PubMed Central  PubMed  Article  Google Scholar 

  • Yan S, Zhao N, Zhang XH (2012) Myroides phaeus sp. nov., isolated from human saliva, and emended descriptions of the genus Myroides and the species Myroides profundi and Myroides marinus. Int J Syst Evol Microbiol 62:770–775

    CAS  PubMed  Article  Google Scholar 

  • Yoon J, Maneerat S, Kawai F, Yokota A (2006) Myroides pelagicus sp. nov., isolated from seawater in Thailand. Int J Syst Evol Microbiol 56(Pt 8):1917–1920

    Article  Google Scholar 

  • Zhang XY, Zhang YJ, Chen XL, Qin QL, Zhao DL, Li TG, Dang HY, Zhang YZ (2008) Myroides profundi sp. nov., isolated from deep-sea sediment of the southern Okinawa Trough. FEMS Microbiol Lett 287(1):108–112

    CAS  PubMed  Article  Google Scholar 

  • Zhang ZD, He LY, Huang Z, Sheng XF (2014) Myroides xuanwuensis sp. nov., a mineral-weathering bacterium isolated from forest soil. Int J Syst Evol Microbiol 64(Pt 2):621–624

    Article  Google Scholar 

Download references


We are grateful to Dr. Jean. P. Euzeby (Society for Systematic and Veterinary Bacteriology, France) for his advice on nomenclature. This work was supported by grants RBM4611423, NMM0101431, and NRF-2013M3A9A5076603, and the KRIBB Research Initiative Program funded by the Ministry of Science, ICT & Future Planning. This work was supported by the Technology Innovation Program, 10046418 (TGM0721311), screening of butyric bacteria and industrial production, funded by the Ministry of Trade, Industry & Energy (MI, Korea).

Author information

Authors and Affiliations


Corresponding author

Correspondence to Young-Hyo Chang.

Additional information

Jayoung Paek and Jeong Hwan Shin have contributed equally to this work.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Paek, J., Shin, J.H., Shin, Y. et al. Myroides injenensis sp. nov., a new member isolated from human urine. Antonie van Leeuwenhoek 107, 201–207 (2015).

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI:


  • Myroides injenensis
  • New species
  • Urine sample