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Variovorax guangxiensis sp. nov., an aerobic, 1-aminocyclopropane-1-carboxylate deaminase producing bacterium isolated from banana rhizosphere

Abstract

A 1-aminocyclopropane-1-carboxylate deaminase producing bacterium, designated GXGD002T, was isolated from the rhizosphere of banana plants cultivated in Guangxi province, China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GXGD002T is a member of the genus Variovorax. High levels of 16S rRNA gene sequence similarity are found between strain GXGD002T and Variovorax paradoxus DSM 30034T (99.4 %), Variovorax ginsengisoli KCTC 12583T (99.1 %), Variovorax boronicumulans KCTC 22010T (99.0 %), Variovorax soli DSM18216T (98.7 %), Variovorax defluvii DSM 27259T (98.1 %) and Variovorax dokdonensis KCTC 12544T (97.4 %) respectively. However, the DNA–DNA hybridization values between strain GXGD002T and its closely related species V. paradoxus DSM 30034T, V. ginsengisoli KCTC 12583T and V. boronicumulans KCTC 22010T were found to be 40.7, 30.9 and 23.7 %, respectively. The DNA G + C content of strain GXGD002T was found to be 67.8 mol%. The major fatty acids of strain GXGD002T are C16:0 (20.3 %), C10:0 3OH (18.4 %), C17:0 cyclo (18.9 %), C18:1w7c (12.3 %) and summed feature 3 (13.9 %). The predominant respiratory quinone was identified as ubiquinone-8 (Q-8) and the major polar lipids as phosphatidylethanolamine and phosphatidylglycerol. The results of polyphasic taxonomic study including physiological and biochemical tests, whole-cell SDS-PAGE profiles and chemotaxonomic analysis allowed a clear differentiation of strain GXGD002T from the other species in the genus Variovorax. Based on these results, a new species, Variovorax guangxiensis, is proposed. The type strain is GXGD002T (=DSM 27352T = ACCC 05911T).

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Acknowledgments

This work was supported by “Special Fund for Agro-scientific Research in the Public Interest” (201203045) .The GenBank accession number for the 16S rRNA gene sequence of strain GXGD002T is JF495126.

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Correspondence to Jian-guang Sun.

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Gao, Jl., Yuan, M., Wang, Xm. et al. Variovorax guangxiensis sp. nov., an aerobic, 1-aminocyclopropane-1-carboxylate deaminase producing bacterium isolated from banana rhizosphere. Antonie van Leeuwenhoek 107, 65–72 (2015). https://doi.org/10.1007/s10482-014-0304-3

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  • DOI: https://doi.org/10.1007/s10482-014-0304-3

Keywords

  • Variovorax guangxiensis sp. nov.
  • Taxonomy
  • 1-Aminocyclopropane-1-carboxylate deaminase
  • Banana rhizosphere