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Sphaerisporangium dianthi sp. nov., an endophytic actinomycete isolated from a root of Dianthus chinensis L

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Abstract

A novel actinomycete, designated strain NEAU-CY18T, was isolated from the root of a Chinese medicinal plant Dianthus chinensis L and subjected to a polyphasic taxonomic study. The novel strain was found to develop spherical sporangia with non-motile spores on aerial mycelium. The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid. The whole-cell sugars were identified as madurose, mannose, ribose, galactose and glucose. The phospholipid profile was found to contain diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylmethylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and an unidentified phospholipid. The predominant menaquinones were identified as MK-9(H4), MK-9(H2) and MK-9(H6). The major fatty acids were identified as C17:0 10-methyl, iso-C16:0 and C16:0. EzTaxon-e analysis of the 16S rRNA gene sequence indicated that the strain belongs to the genus Sphaerisporangium and was most closely related to Sphaerisporangium cinnabarinum JCM 3291T (98.9 %) and Sphaerisporangium melleum JCM 13064T (98.3 %). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-CY18T forms a monophyletic clade with S. cinnabarinum JCM 3291T, an association that was supported by a bootstrap value of 97 % in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. Comparisons of some phenotypic properties and low DNA–DNA relatedness values enabled the strain to be differentiated from S. cinnabarinum JCM 3291T and S. melleum JCM 13064T. Therefore, it is concluded that strain NEAU-CY18T represents a novel Sphaerisporangium species, for which the name Sphaerisporangium dianthi sp. nov. is proposed. The type strain is NEAU-CY18T ( = CGMCC 4.7132T = DSM 46736T).

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Acknowledgments

This work was supported in part by grants from the National Outstanding Youth Foundation (No. 31225024), the National Key Project for Basic Research (No. 2010CB126102), the National Key Technology R&D Program (No. 2012BAD19B06), the Program for New Century Excellent Talents in University (NCET-11-0953), the National Natural Science Foundation of China (No. 31372006, 31171913, 31071750 and 31471832), the Outstanding Youth Foundation of Heilongjiang Province (JC201201), the Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (CSCP), the Science and Technology Research Project of Heilongjiang Provincial Educational Commission (No. 12541001) and the Youth Science Foundation of Heilongjiang Province (No. QC2014C013).

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Authors and Affiliations

  1. Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People’s Republic of China

    Jia Xing, Chongxi Liu, Yuejing Zhang, Ying Zhou, Lianjie Li, Junwei Zhao, Shuanghe Liu, Xiangjing Wang & Wensheng Xiang

  2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China

    Hairong He & Wensheng Xiang

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  1. Jia Xing
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  2. Chongxi Liu
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  3. Yuejing Zhang
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  8. Shuanghe Liu
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  9. Xiangjing Wang
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Correspondence to Xiangjing Wang or Wensheng Xiang.

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Xing, J., Liu, C., Zhang, Y. et al. Sphaerisporangium dianthi sp. nov., an endophytic actinomycete isolated from a root of Dianthus chinensis L. Antonie van Leeuwenhoek 107, 9–14 (2015). https://doi.org/10.1007/s10482-014-0298-x

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