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Actinotalea suaedae sp. nov., isolated from the halophyte Suaeda physophora in Xinjiang, Northwest China

Antonie van Leeuwenhoek volume 107, pages 1–7 (2015)Cite this article

Abstract

A Gram-stain-positive, aerobic, non-motile, coryneform bacterium, designated strain EGI 60002T, was isolated from the halophyte Suaeda physophora. Cells were coryneform shaped and polymorphic. Phylogenetic analysis based on 16S rRNA gene sequences showed that the new isolate was closely related to Actinotalea ferrariae CF5-4T (95.8 % gene sequence similarity). The peptidoglycan type of strain EGI 60002T was A4β, containing l-Orn-d-Ser-d-Asp. The cell-wall sugars were mannose, ribose, rhamnose and glucose. The major fatty acids (>5 %) of strain EGI 60002T were iso-C14:0, iso-C15:0, anteiso-C15:1 A and anteiso-C15:0. The predominant respiratory quinone was MK-10(H4). The major polar lipids were diphosphatidylglycerol (DPG), one unidentified phosphoglycolipid (PGL) and one unidentified phospholipid (PL1). The genomic DNA G+C content was 72.3 mol%. On the basis of morphological, physiological, chemotaxonomic data, and phylogenetic analysis, strain EGI 60002T should be classified as a novel species within the genus Actinotalea, for which the name Actinotalea suaedae sp. nov. is proposed. The type strain is EGI 60002T (=JCM 19624T = KACC 17839T = KCTC 29256T).

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Acknowledgments

The authors are grateful to Prof. Ge-Jiao Wang (Huazhong Agricultural University, Wuhan, China) for her kind providing reference type strains, and Prof. Aharon Oren (The Hebrew University of Jerusalem, Israel) for his kind help with the Latin etymology for the new species. This work was supported by the National Natural Science Foundation of China (Nos. 31200008, 31300432 and 31400009) and State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University (CSBAA2013002), and the West Light Foundation of the Chinese Academy of Sciences and the Hundred Talents Program of the Chinese Academy of Sciences. The authors extend their appreciation to the Deanship of Scientific Research at King Saud University for funding this work through the research group no RGP-205.

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Authors and Affiliations

  1. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, 830011, People’s Republic of China

    Shuai Zhao, Li Li, Hong-Fei Wang, Yong-Guang Zhang, Wen-Jun Li & Chang-Yan Tian

  2. State Key Laboratory of Biocontrol, Key Laboratory of Biodiversity Dynamics and Conservation of Guangdong Higher Education Institutes, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Shan-Hui Li & Wen-Jun Li

  3. Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Shan-Hui Li & Wen-Jun Li

  4. Bioproducts Research Chair (BRC), College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia

    Wael N. Hozzein & Mohammed A. M. Wadaan

  5. State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, 830011, People’s Republic of China

    Shuai Zhao & Chang-Yan Tian

  6. State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Li Li

  7. University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Shuai Zhao & Hong-Fei Wang

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  1. Shuai Zhao
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  2. Li Li
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  3. Shan-Hui Li
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  4. Hong-Fei Wang
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  5. Wael N. Hozzein
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  6. Yong-Guang Zhang
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  7. Mohammed A. M. Wadaan
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  8. Wen-Jun Li
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  9. Chang-Yan Tian
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Corresponding authors

Correspondence to Wen-Jun Li or Chang-Yan Tian.

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Shuai Zhao and Li Li have contributed equally to this work.

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Zhao, S., Li, L., Li, SH. et al. Actinotalea suaedae sp. nov., isolated from the halophyte Suaeda physophora in Xinjiang, Northwest China. Antonie van Leeuwenhoek 107, 1–7 (2015). https://doi.org/10.1007/s10482-014-0297-y

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Keywords

  • Actinotalea suaedae sp. nov.
  • Endophytic bacteria
  • Halophyte
  • Suaeda physophora