Antonie van Leeuwenhoek

, Volume 106, Issue 6, pp 1259–1267 | Cite as

Oenococcus alcoholitolerans sp. nov., a lactic acid bacteria isolated from cachaça and ethanol fermentation processes

  • Fernanda Badotti
  • Ana Paula B. Moreira
  • Luciane A. Chimetto Tonon
  • Brígida T. Luckwu de Lucena
  • Fátima de Cássia O. Gomes
  • Ricardo Kruger
  • Cristiane C. Thompson
  • Marcos Antonio de MoraisJr.
  • Carlos A. Rosa
  • Fabiano L. Thompson
Original Paper


Four strains of lactic acid bacteria isolated from cachaça and alcohol fermentation vats in Brazil were characterised in order to determine their taxonomic position. Phylogenetic analysis revealed that they belong to the genus Oenococcus and should be distinguished from their closest neighbours. The 16S rRNA gene sequence similarity against the type strains of the other two species of the genus was below 94.76 % (Oenococcus kitaharae) and 94.62 % (Oenococcus oeni). The phylogeny based on pheS gene sequences also confirmed the position of the new taxon. DNA–DNA hybridizations based on in silico genome-to-genome comparison, Average Amino Acid Identity, Average Nucleotide Identity and Karlin genomic signature confirmed the novelty of the taxon. Distinctive phenotypic characteristics are the ability to metabolise sucrose but not trehalose. The name Oenococcus alcoholitolerans sp. nov. is proposed for this taxon, with the type strain UFRJ-M7.2.18T ( = CBAS474T = LMG27599T). In addition, we have determined a draft genome sequence of the type strain.


Oenococcus alcoholitolerans Cachaça Ethanol Genomic taxonomy 



Financial support came from Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG), Fundação de Amparo a Pesquisa do Estado de Pernambuco (FACEPE), Fundação de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

Supplementary material

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Supplementary material 1 (DOC 48 kb)
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Supplementary material 7 (PDF 257 kb)


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Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • Fernanda Badotti
    • 1
  • Ana Paula B. Moreira
    • 2
  • Luciane A. Chimetto Tonon
    • 2
  • Brígida T. Luckwu de Lucena
    • 3
  • Fátima de Cássia O. Gomes
    • 4
  • Ricardo Kruger
    • 5
  • Cristiane C. Thompson
    • 2
  • Marcos Antonio de MoraisJr.
    • 6
  • Carlos A. Rosa
    • 1
  • Fabiano L. Thompson
    • 7
  1. 1.Departamento de Microbiologia, Instituto de Ciências Biológicas (ICB)Universidade Federal de Minas GeraisBelo HorizonteBrazil
  2. 2.Instituto de BiologiaUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil
  3. 3.Centro de Ciências Biológicas e Sociais AplicadasUniversidade Estadual da ParaíbaJoão PessoaBrazil
  4. 4.Departamento de QuímicaCentro Federal de Educação Tecnológica de Minas GeraisBelo HorizonteBrazil
  5. 5.Laboratório de Enzimologia, Departamento de Biologia Celular, Instituto de BiologiaUniversidade de BrasíliaBrasíliaBrazil
  6. 6.Departamento de GenéticaUniversidade Federal de PernambucoRecifeBrazil
  7. 7.Instituto de Biologia and Laboratório de Sistemas Avançados de Gestão da Produção - SAGE/ COPPEUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil

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