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Bizionia psychrotolerans sp. nov., a psychrophilic bacterium isolated from the intestine of a sea cucumber (Apostichopus japonicus)

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Abstract

A novel Gram-negative, non-flagellated, non-gliding, rod-shaped bacterial strain, designated PB-M7T, was isolated from the intestine of a sea cucumber collected from Pohang, South Korea. Growth was observed at 4–30 °C (optimum, 25 °C), pH 6.0–9.0 (optimum, pH 7.0–8.0), and with 2.0–6.0 % (w/v) NaCl (optimum, 2.0 %). In a phylogenetic analysis based on 16S rRNA gene sequences, strain PB-M7T was found to belong to the genus Bizionia and to be most closely related to Bizionia echini KMM 6177T (99.0 % 16S rRNA gene sequence similarity), Bizionia hallyeonensis T-y7T (97.9 %), Bizionia algoritergicola APA-1T (97.5 %), Bizionia argentinensis JUB59T (97.5 %) and Bizionia myxarmorum ADA-4T (97.1 %). The predominant fatty acids of strain PB-M7T were identified as iso-C15:0 (22.2 %), iso-C15:1 G (10.8 %), iso-C17:0 3-OH (16.7 %) and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c; 11.2 %). The major respiratory quinone was identified as menaquinone-6 (MK-6). The polar lipid profile of strain PB-M7T was found to contain phosphatidylethanolamine, an unidentified phospholipid, three unidentified aminolipids and three unidentified lipids. The DNA G + C content of strain PB-M7T was determined to be 33.4 mol% and the mean DNA–DNA relatedness values with the type strains of B. echini, B. hallyeonensis, B. algoritergicola, B. argentinensis, and B. myxarmorum were 52.9, 48.5, 46.5, 37.1 and 26.6 %, respectively. Based on the data presented, strain PB-M7T represents a novel species of the genus Bizionia, for which the name Bizionia psychrotolerans sp. nov. is proposed. The type strain of B. psychrotolerans is PB-M7T (= KCCM 43042 T = JCM 19924 T).

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Authors and Affiliations

  1. Fermentation Research Center, Korea Food Research Institute (KFRI), Seongnam, 463-746, Republic of Korea

    Eun-Ji Song, Mi-Hwa Lee, So-lim Park & Young-Do Nam

  2. Korea University of Science and Technology, Daejeon, 305-350, Republic of Korea

    Eun-Ji Song, Seong Woon Roh & Young-Do Nam

  3. Division of Bioengineering, University of Incheon, Incheon, 406-772, Republic of Korea

    Myung-Ji Seo

  4. Jeju Center, Korea Basic Science Institute, Jeju, 690-140, Republic of Korea

    Kyung June Yim & Seong Woon Roh

  5. Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul, 130-701, Republic of Korea

    Dong-Wook Hyun & Jin-Woo Bae

Authors
  1. Eun-Ji Song
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  2. Mi-Hwa Lee
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  3. Myung-Ji Seo
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  4. Kyung June Yim
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  6. Jin-Woo Bae
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  7. So-lim Park
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  8. Seong Woon Roh
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  9. Young-Do Nam
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Correspondence to Seong Woon Roh or Young-Do Nam.

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Eun-Ji Song and Mi-Hwa Lee have contributed equally to this work.

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Song, EJ., Lee, MH., Seo, MJ. et al. Bizionia psychrotolerans sp. nov., a psychrophilic bacterium isolated from the intestine of a sea cucumber (Apostichopus japonicus). Antonie van Leeuwenhoek 106, 837–844 (2014). https://doi.org/10.1007/s10482-014-0253-x

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  • DOI: https://doi.org/10.1007/s10482-014-0253-x

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