Skip to main content

Sinomonas notoginsengisoli sp. nov., isolated from the rhizosphere of Panax notoginseng

Antonie van Leeuwenhoek volume 106, pages 827–835 (2014)Cite this article

Abstract

A Gram-positive, aerobic, non-motile and pale yellow colour actinobacterial strain, designated SYP-B575T, was isolated from rhizosphere soil of Panax notoginseng. The optimal growth of the strain was found to occur at 28 °C, pH 7.0 and without NaCl. Phylogenetic analysis indicated that strain SYP-B575T clearly belongs to the genus Sinomonas and should be considered as a candidate of novel species within this genus. The 16S rRNA gene sequence similarities between strain SYP-B575T and the other Sinomonas type strains ranged from 97.3 to 96.0 %. The predominant isoprenoid quinone was identified as MK-9(H2) and the major fatty acids were identified as anteiso-C15:0 and anteiso-C17:0. The polar lipids were found to consist of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and glycolipids. The major cell-wall amino acids were identified as Lys, Ala, Glu, Gly and Ser. The whole-cell sugars were identified as mannose, ribose, rhamnose, glucose and galactose. The G+C content of the genomic DNA was determined to be 66.6 mol%. The DNA–DNA relatedness values between SYP-B575T and its closest phylogenetic neighbours were lower than 35.5 %. On the basis of this polyphasic taxonomic study, strain SYP-B575T represents a novel species of the genus Sinomonas, for which the name Sinomonas notoginsengisoli sp. nov. is proposed. The type strain is SYP-B575T (=DSM 27685T = KCTC 29237T).

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Fig. 1
Fig. 2
Fig. 3

References

  • Adesemoye AO, Kloepper JW (2009) Plant–microbes interactions in enhanced fertilizer use efficiency. Appl Microbiol Biotechnol 85:1–12

    CAS  PubMed  Article  Google Scholar 

  • Ahmad F, Ahmad I, Khan M (2008) Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiol Res 163:173–181

    CAS  PubMed  Article  Google Scholar 

  • Barea JM, Azcón R, Azcón AC (2002) Mycorrhizosphere interactions to improve plant fitness and soil quality. Anton Leeuw Int J G 81:343–351

    CAS  Article  Google Scholar 

  • Bhattacharyya P, Jha DK (2012) Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. World J Microbiol Biotechnol 28:1327–1350

    CAS  PubMed  Article  Google Scholar 

  • Chan P (2002) Protective effects of trilinolein extrated from Panax notoginseng against cardiovascular disease. Acta Pharmacol Sin 23:1157–1162

    CAS  PubMed  Google Scholar 

  • Choi RCY, Zhu JTT, Leung KW, Chu GKY, Xie HQ, Chen VP, Zheng KYZ, Lau DTW, Dong TTX, Chow PCY, Han YF, Wang ZT, Tsim KWK (2010) A flavonol glycoside, isolated from roots of Panax notoginseng, reduces amyloid-β-induced neurotoxicity in cultured neurons: signaling transduction and drug development for Alzheimer’s disease. J Alzheimers Dis 19:795–811

    CAS  PubMed  Google Scholar 

  • Cicero AFG, Vitale G, Savino G, Arletti R (2003) Panax notoginseng (Burk.) effects on fibrinogen and lipid plasma level in rats fed on a high-fat diet. Phytother Res 17:174–178

    CAS  PubMed  Article  Google Scholar 

  • Ding L, Hirose T, Yokota A (2009) Four novel Arthrobacter species isolated from filtration substrate. Int J Syst Evol Microbiol 59:856–862

    CAS  PubMed  Article  Google Scholar 

  • Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229

    Article  Google Scholar 

  • Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376

    CAS  PubMed  Article  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Gonzalez C, Gutierrez C, Ramirez C (1978) Halobacterium vallismortis sp. nov., an amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 24:710–715

    CAS  PubMed  Article  Google Scholar 

  • Hu HY, Lim BR, Goto N, Fujie K (2001) Analytical precision and repeatability of respiratory quinones for quantitative study of microbial community structure in environmental samples. J Microbiol Method 47:17–24

    CAS  Article  Google Scholar 

  • Kelly KL (1964) Inter-Society Color Council-National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. US Government Printing Office, Washington, DC

    Google Scholar 

  • Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon Y, Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721

    CAS  PubMed  Article  Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    CAS  PubMed  Article  Google Scholar 

  • Kuhn DA, Starr MP (1960) Arthrobacter atrocyaneus, nov. sp., and its blue pigment. Arch Microbiol 36:175–181

    CAS  Google Scholar 

  • Li WJ, Xu P, Schumann P, Zhang YQ, Pukall R, Xu LH, Stackebrandt E, Jiang CL (2007) Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57:1424–1428

    PubMed  Article  Google Scholar 

  • Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167

    CAS  Article  Google Scholar 

  • Minnikin D, O’donnell A, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett J (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Method 2:233–241

    CAS  Article  Google Scholar 

  • Ng TB (2006) Pharmacological activity of sanchi ginseng (Panax notoginseng). J Pharm Pharmacol 58:1007–1019

    CAS  PubMed  Article  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  • Schleifer KH, Kandler O (1972) Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477

    CAS  PubMed Central  PubMed  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    CAS  PubMed Central  PubMed  Article  Google Scholar 

  • Tang SK, Wang Y, Chen Y, Lou K, Cao LL, Xu LH, Li WJ (2009) Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella. Int J Syst Evol Microbiol 59:2025–2032

    CAS  PubMed  Article  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    CAS  PubMed Central  PubMed  Article  Google Scholar 

  • Wang CZ, Luo X, Zhang B, Song WX, Ni M, Mehendale S, Xie JT, Aung HH, He TC, Yuan CS (2007) Notoginseng enhances anti-cancer effect of 5-fluorouracil on human colorectal cancer cells. Cancer Chemother Pharmacol 60:69–79

    PubMed Central  PubMed  Article  Google Scholar 

  • Wayne LG, Brenner DJ, Colwell RR et al (1987) Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464

  • Wieser M, Denner EB, Kämpfer P, Schumann P, Tindall B, Steiner U, Vybiral D, Lubitz W, Maszenan A, Patel B (2002) Emended descriptions of the genus Micrococcus, Micrococcus luteus (Cohn 1872) and Micrococcus lylae (Kloos et al. 1974). Int J Syst Evol Microbiol 52:629–637

  • Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ, Chen HH, Xu H, Jiang CL (2005) Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family Oxalobacteraceae isolated from China. Int J Syst Evol Microbiol 55:1149–1153

    CAS  PubMed  Article  Google Scholar 

  • Zhou Y, Wei W, Wang X, Lai R (2009) Proposal of Sinomonas flava gen. nov., sp. nov., and description of Sinomonas atrocyanea comb. nov. to accommodate Arthrobacter atrocyaneus. Int J Syst Evol Microbiol 59:259–263

    CAS  PubMed  Article  Google Scholar 

  • Zhou Y, Chen X, Zhang Y, Wang W, Xu J (2012) Description of Sinomonas soli sp. nov., reclassification of Arthrobacter echigonensis and Arthrobacter albidus (Ding et al. 2009) as Sinomonas echigonensis comb. nov. and Sinomonas albida comb. nov., respectively, and emended description of the genus Sinomonas. Int J Syst Evol Microbiol 62:764–769

Download references

Acknowledgments

The authors are grateful to Prof. Dr. Aharon Oren (The Hebrew University of Jerusalem, Israel) for his kind help with the Latin etymology for the new species, Prof. Dr. Hans-Peter Klenk (DSMZ, Germany) and Dr. Yu Zhou (Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, China) for their kind providing the reference type strains. This research was supported by Key Project of Yunnan Provincial Natural Science Foundation (2013FC008). WJ Li was also supported by Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme (2014).

Author information

Authors and Affiliations

  1. School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, 110016, People’s Republic of China

    Meng-Yue Zhang, Jun Xie, Tian-Yuan Zhang, Huan Xu & Yi-Xuan Zhang

  2. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Juan Cheng, Shan-Hui Li & Wen-Jun Li

  3. State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Wen-Jun Li

Authors
  1. Meng-Yue Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jun Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tian-Yuan Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Huan Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Juan Cheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Shan-Hui Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Wen-Jun Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Yi-Xuan Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Wen-Jun Li or Yi-Xuan Zhang.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 551 kb)

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Zhang, MY., Xie, J., Zhang, TY. et al. Sinomonas notoginsengisoli sp. nov., isolated from the rhizosphere of Panax notoginseng . Antonie van Leeuwenhoek 106, 827–835 (2014). https://doi.org/10.1007/s10482-014-0252-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10482-014-0252-y

Keywords

  • Sinomonas notoginsengisoli sp. nov.
  • Rhizosphere
  • Panax notoginseng
  • Polyphasic taxonomy