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Sinomonas notoginsengisoli sp. nov., isolated from the rhizosphere of Panax notoginseng

Abstract

A Gram-positive, aerobic, non-motile and pale yellow colour actinobacterial strain, designated SYP-B575T, was isolated from rhizosphere soil of Panax notoginseng. The optimal growth of the strain was found to occur at 28 °C, pH 7.0 and without NaCl. Phylogenetic analysis indicated that strain SYP-B575T clearly belongs to the genus Sinomonas and should be considered as a candidate of novel species within this genus. The 16S rRNA gene sequence similarities between strain SYP-B575T and the other Sinomonas type strains ranged from 97.3 to 96.0 %. The predominant isoprenoid quinone was identified as MK-9(H2) and the major fatty acids were identified as anteiso-C15:0 and anteiso-C17:0. The polar lipids were found to consist of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and glycolipids. The major cell-wall amino acids were identified as Lys, Ala, Glu, Gly and Ser. The whole-cell sugars were identified as mannose, ribose, rhamnose, glucose and galactose. The G+C content of the genomic DNA was determined to be 66.6 mol%. The DNA–DNA relatedness values between SYP-B575T and its closest phylogenetic neighbours were lower than 35.5 %. On the basis of this polyphasic taxonomic study, strain SYP-B575T represents a novel species of the genus Sinomonas, for which the name Sinomonas notoginsengisoli sp. nov. is proposed. The type strain is SYP-B575T (=DSM 27685T = KCTC 29237T).

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Acknowledgments

The authors are grateful to Prof. Dr. Aharon Oren (The Hebrew University of Jerusalem, Israel) for his kind help with the Latin etymology for the new species, Prof. Dr. Hans-Peter Klenk (DSMZ, Germany) and Dr. Yu Zhou (Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, China) for their kind providing the reference type strains. This research was supported by Key Project of Yunnan Provincial Natural Science Foundation (2013FC008). WJ Li was also supported by Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme (2014).

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Correspondence to Wen-Jun Li or Yi-Xuan Zhang.

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Zhang, MY., Xie, J., Zhang, TY. et al. Sinomonas notoginsengisoli sp. nov., isolated from the rhizosphere of Panax notoginseng . Antonie van Leeuwenhoek 106, 827–835 (2014). https://doi.org/10.1007/s10482-014-0252-y

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Keywords

  • Sinomonas notoginsengisoli sp. nov.
  • Rhizosphere
  • Panax notoginseng
  • Polyphasic taxonomy