Bacillus haikouensis sp. nov., a facultatively anaerobic halotolerant bacterium isolated from a paddy soil

Abstract

A Gram-stain positive, rod-shaped, endospore-forming and facultatively anaerobic halotolerant bacterium, designated as C-89T, was isolated from a paddy field soil in Haikou, Hainan Province, People’s Republic of China. Optimal growth was observed at 37 °C and pH 7.0 in the presence of 4 % NaCl (w/v). The predominant menaquinone was identified as MK-7, the major cellular fatty acids were identified as anteiso-C15:0 and iso-C15:0, and the major cellular polar lipids were identified as phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and two unknown phospholipids. The peptidoglycan type was determined to be based on meso-DAP. Based on 16S rRNA gene sequence similarity, the closest phylogenetic relatives were identified as Bacillus vietnamensis JCM 11124T (98.8 % sequence similarity), Bacillus aquimaris JCM 11545T (98.6 %) and Bacillus marisflavi JCM 11544T (98.5 %). The DNA G+C content of strain C-89T was determined to be 45.4 mol%. The DNA–DNA relatedness values of strain C-89T with its closest relatives were below 18 %. Therefore, on the basis of phylogenetic, chemotaxonomic, and phenotypic results, strain C-89T can be considered to represent a novel species within the genus Bacillus, for which the name Bacillus haikouensis sp. nov., is proposed. The type strain is C-89T (=KCTC 33545T = CCTCC AB 2014076T).

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References

  1. Ash C, Farrow JA, Dorsch M, Stackebrandt E, Collins MD (1991) Comparative analysis of Bacillus anthracis, Bacillus cereus, and related species on the basis of reverse transcriptase sequencing of 16S rRNA. Int J Syst Bacteriol 41:343–346

    CAS  PubMed  Article  Google Scholar 

  2. Baker G, Smith J, Cowan DA (2003) Review and re-analysis of domain-specific 16S primers. J Microbiol Methods 55:541–555

    CAS  PubMed  Article  Google Scholar 

  3. Collins M, Jones D (1981) Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. Microbiol Rev 45:316

    CAS  PubMed Central  PubMed  Google Scholar 

  4. Collins M, Pirouz T, Goodfellow M, Minnikin D (1977) Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230

    CAS  PubMed  Article  Google Scholar 

  5. Dong X, Cai M (2001) Manual of systematic and determinative bacteriology. Science Press, Beijing

    Google Scholar 

  6. Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229

    Article  Google Scholar 

  7. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  8. Kämpfer P (1994) Limits and possibilities of total fatty acid analysis for classification and identification of bacillus species. Syst Appl Microbiol 17:86–98

    Article  Google Scholar 

  9. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721

    CAS  PubMed  Article  Google Scholar 

  10. Lee JC, Lim JM, Park DJ, Jeon CO, Li WJ, Kim CJ (2006) Bacillus seohaeanensis sp nov., a halotolerant bacterium that contains l-lysine in its cell wall. Int J Syst Evol Microbiol 56:1893–1898

    CAS  PubMed  Article  Google Scholar 

  11. Li J, Yang G, Wu M, Zhao Y, Zhou S (2014) Bacillus huizhouensis sp. nov., isolated from a paddy field soil. Antonie Van Leeuwenhoek 106:357–363

    CAS  PubMed  Article  Google Scholar 

  12. Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167

    CAS  Article  Google Scholar 

  13. Minnikin D, Patel P, Alshamaony L, Goodfellow M (1977) Polar lipid composition in the classification of nocardia and related bacteria. Int J Syst Bacteriol 27:104–117

    CAS  Article  Google Scholar 

  14. Noguchi H, Uchino M, Shida O, Takano K, Nakamura LK, Komagata K (2004) Bacillus vietnamensis sp nov., a moderately halotolerant, aerobic, endospore-forming bacterium isolated from Vietnamese fish sauce. Int J Syst Evol Microbiol 54:2117–2120

    CAS  PubMed  Article  Google Scholar 

  15. Rzhetsky A, Nei M (1993) Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol 10:1073–1095

    CAS  PubMed  Google Scholar 

  16. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  17. Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. Technical Note 101. Microbial ID, Newark, Delaware

  18. Schleifer KH (1985) Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156

    CAS  Article  Google Scholar 

  19. Shida O, Takagi H, Kadowaki K, Nakamura LK, Komagata K (1997) Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus. Int J Syst Bacteriol 47:289–298

    CAS  PubMed  Article  Google Scholar 

  20. Tamaoka J, Katayama-Fujimura Y, Kuraishi H (1983) Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Microbiol 54:31–36

    CAS  Google Scholar 

  21. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    CAS  PubMed  Article  Google Scholar 

  22. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    CAS  PubMed Central  PubMed  Article  Google Scholar 

  23. Wainø M, Tindall BJ, Schumann P, Ingvorsen K (1999) Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov. Int J Syst Bacteriol 49:821–831

    PubMed  Article  Google Scholar 

  24. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE (1987) International committee on systematic bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464

    Article  Google Scholar 

  25. Yoon JH, Kim IG, Kang KH, Oh TK, Park YH (2003) Bacillus marisflavi sp. nov. and Bacillus aquimaris sp. nov., isolated from sea water of a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 53:1297–1303

    CAS  PubMed  Article  Google Scholar 

  26. Zhang L, Wang Y, Dai J, Tang Y, Yang Q, Luo X, Fang C (2009) Bacillus korlensis sp. nov., a moderately halotolerant bacterium isolated from a sand soil sample in China. Int J Syst Evol Microbiol 59:1787–1792

    CAS  PubMed  Article  Google Scholar 

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Acknowledgments

This work was supported by the Team Project of Guangdong Natural Science Foundation (S2012030006144) and The Science and Technology Planning Project of Guangdong Province, China (2012B091100271).

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Correspondence to Shungui Zhou.

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Li, J., Yang, G., Lu, Q. et al. Bacillus haikouensis sp. nov., a facultatively anaerobic halotolerant bacterium isolated from a paddy soil. Antonie van Leeuwenhoek 106, 789–794 (2014). https://doi.org/10.1007/s10482-014-0248-7

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Keywords

  • Bacillus haikouensis sp. nov.
  • Gram-stain positive
  • Halotolerant