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Mariniflexile ostreae sp. nov., a member of the family Flavobacteriaceae isolated from an oyster

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Abstract

A Gram-negative, aerobic, non-flagellated and rod-shaped bacterial strain able to move by gliding, designated TYO-10T, was isolated from an oyster collected at Tongyoung on the South Sea, South Korea. Strain TYO-10T was found to grow optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain TYO-10T joined the cluster comprising the type strains of Mariniflexile species. Strain TYO-10T exhibited 16S rRNA gene sequence similarity values of 95.4–95.7 % to the type strains of Mariniflexile species and of less than 95.4 % to the type strains of other recognized species. Strain TYO-10T was found to contain MK-6 as the predominant menaquinone and iso-C15:0, iso-C15:1 G, anteiso-C15:0, iso-C17:0 3-OH and iso-C15:0 3-OH as the major fatty acids. The major polar lipids of strain TYO-10T were identified as phosphatidylethanolamine and an unidentified lipid, which is similar to those of the type strains of Mariniflexile species, but different from those of other phylogenetically related species. The DNA G+C content of strain TYO-10T was determined to be 35.9 mol%. The differential phenotypic properties, together with the phylogenetic distinctiveness, revealed that strain TYO-10T is separated from other Mariniflexile species. On the basis of the data presented, strain TYO-10T is considered to represent a novel species of the genus Mariniflexile, for which the name Mariniflexile ostreae sp. nov. is proposed. The type strain is TYO-10T (= KCTC 42113T = CECT 8622T).

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Acknowledgments

This work was supported by the project on survey of indigenous species of Korea of the National Institute of Biological Resources (NIBR) under the Ministry of Environment (MOE) and the Program for Collection, Management and Utilization of Biological Resources (grant NRF-2013M3A9A5075953) from the Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea

    Sooyeon Park, Chul-Hyung Kang, Ja-Min Park & Jung-Hoon Yoon

  2. University of Science and Technology (UST), 113 Gwahangno, Yuseong, Daejeon, South Korea

    Chul-Hyung Kang

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  1. Sooyeon Park
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  2. Chul-Hyung Kang
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Correspondence to Jung-Hoon Yoon.

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Park, S., Kang, CH., Park, JM. et al. Mariniflexile ostreae sp. nov., a member of the family Flavobacteriaceae isolated from an oyster. Antonie van Leeuwenhoek 106, 555–563 (2014). https://doi.org/10.1007/s10482-014-0227-z

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