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Thalassobius aquaeponti sp. nov., an alphaproteobacterium isolated from seawater

Abstract

A Gram-stain-negative, aerobic, non-motile and rod-shaped or ovoid bacterial strain, GJSW-22T, which was isolated from seawater at Geoje island in South Korea, was characterized taxonomically. Strain GJSW-22T was observed to grow optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain GJSW-22T grouped with the type strains of Thalassobius species, forming a stable cluster with the type strain of Thalassobius aestuarii (bootstrap value of 83.2 %). Strain GJSW-22T exhibited the highest 16S rRNA gene sequence similarity value (98.0 %) to the type strain of T. aestuarii. It exhibited 16S rRNA gene sequence similarity values of 95.6–96.1 % to the type strains of the other Thalassobius species. Strain GJSW-22T was found to contain Q-10 as the predominant ubiquinone and C18:1 ω7c and 11-methyl C18:1 ω7c as the major fatty acids. The major polar lipids of strain GJSW-22T were identified as phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid. The DNA G + C content of strain GJSW-22T is 60.3 mol % and its mean DNA–DNA relatedness value with the type strain of T. aestuarii was 23 %. Differential phenotypic properties, together with the phylogenetic and chemotaxonomic data, confirmed that strain GJSW-22T is distinct from other Thalassobius species. On the basis of the data presented, strain GJSW-22T is considered to represent a novel species of the genus Thalassobius, for which the name Thalassobius aquaeponti sp. nov. is proposed. The type strain is GJSW-22T (=KCTC 42115T = NBRC 110378T).

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Acknowledgments

This work was supported by the Program for Collection, Management and Utilization of Biological Resources (grant NRF-2013M3A9A5075953) from the Ministry of Science, ICT and Future Planning (MSIP) of the Republic of Korea.

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Correspondence to Jung-Hoon Yoon.

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Park, S., Jung, YT., Won, SM. et al. Thalassobius aquaeponti sp. nov., an alphaproteobacterium isolated from seawater. Antonie van Leeuwenhoek 106, 535–542 (2014). https://doi.org/10.1007/s10482-014-0225-1

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  • DOI: https://doi.org/10.1007/s10482-014-0225-1

Keywords

  • Seawater
  • Polyphasic taxonomy
  • Novel species
  • Thalassobius aquaeponti