Skip to main content
SpringerLink
Log in

Thalassobius aquaeponti sp. nov., an alphaproteobacterium isolated from seawater

Antonie van Leeuwenhoek Aims and scope Submit manuscript

Cite this article

Abstract

A Gram-stain-negative, aerobic, non-motile and rod-shaped or ovoid bacterial strain, GJSW-22T, which was isolated from seawater at Geoje island in South Korea, was characterized taxonomically. Strain GJSW-22T was observed to grow optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain GJSW-22T grouped with the type strains of Thalassobius species, forming a stable cluster with the type strain of Thalassobius aestuarii (bootstrap value of 83.2 %). Strain GJSW-22T exhibited the highest 16S rRNA gene sequence similarity value (98.0 %) to the type strain of T. aestuarii. It exhibited 16S rRNA gene sequence similarity values of 95.6–96.1 % to the type strains of the other Thalassobius species. Strain GJSW-22T was found to contain Q-10 as the predominant ubiquinone and C18:1 ω7c and 11-methyl C18:1 ω7c as the major fatty acids. The major polar lipids of strain GJSW-22T were identified as phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid. The DNA G + C content of strain GJSW-22T is 60.3 mol % and its mean DNA–DNA relatedness value with the type strain of T. aestuarii was 23 %. Differential phenotypic properties, together with the phylogenetic and chemotaxonomic data, confirmed that strain GJSW-22T is distinct from other Thalassobius species. On the basis of the data presented, strain GJSW-22T is considered to represent a novel species of the genus Thalassobius, for which the name Thalassobius aquaeponti sp. nov. is proposed. The type strain is GJSW-22T (=KCTC 42115T = NBRC 110378T).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Arahal DR, Macián MC, Garay E, Pujalte MJ (2005) Thalassobius mediterraneus gen. nov., sp. nov., and reclassification of Ruegeria gelatinovorans as Thalassobius gelatinovorus comb. nov. Int J Syst Evol Microbiol 55:2371–2376

    Article  CAS  PubMed  Google Scholar 

  • Bruns A, Rohde M, Berthe-Corti L (2001) Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006

    Article  CAS  PubMed  Google Scholar 

  • Chen MH, Sheu SY, Chen CA, Wang JT, Chen WM (2011) Shimia isoporae sp. nov., isolated from the reefbuilding coral Isopora palifera. Int J Syst Evol Microbiol 61:823–827

    Article  CAS  PubMed  Google Scholar 

  • Choi DH, Cho BC (2006) Shimia marina gen. nov., sp. nov., a novel bacterium of the Roseobacter clade isolated from biofilm in a coastal fish farm. Int J Syst Evol Microbiol 56:1869–1873

    Article  CAS  PubMed  Google Scholar 

  • Euzéby JP (1997) List of bacterial names with standing in nomenclature: a folder available on the internet. Int J Syst Bacteriol 47:590–592 (List of Prokaryotic names with Standing in Nomenclature. URL: http://www.bacterio.net)

  • Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229

    Article  Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  • Hameed A, Shahina M, Lin SY, Lai WA, Hsu YH, Liu YC, Huang YM, Young CC (2013) Shimia biformata sp. nov., isolated from surface seawater, and emended description of the genus Shimia Choi and Cho 2006. Int J Syst Evol Microbiol 63:4533–4539

    Article  CAS  PubMed  Google Scholar 

  • Hyun DW, Kim MS, Shin NR, Kim JY, Kim PS, Whon TW, Yun JH, Bae JW (2013) Shimia haliotis sp. nov., a bacterium isolated from the gut of an abalone, Haliotis discus hannai. Int J Syst Evol Microbiol 63:4248–4253

    Article  CAS  PubMed  Google Scholar 

  • Komagata K, Suzuki KI (1987) Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19:161–207

    Article  CAS  Google Scholar 

  • Minnikin DE, Patel PV, Alshamaony L, Goodfellow M (1977) Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117

    Article  CAS  Google Scholar 

  • Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241

    Article  CAS  Google Scholar 

  • Park S, Yoon JH (2014) Octadecabacter jejudonensis sp. nov., isolated from the junction between the ocean and a freshwater spring and emended description of the genus Octadecabacter. Int J Syst Evol Microbiol 64:719–724

    Article  PubMed  Google Scholar 

  • Park S, Lee MH, Lee JS, Oh TK, Yoon JH (2012) Thalassobius maritimus sp. nov., isolated from seawater. Int J Syst Evol Microbiol 62:8–12

    Article  CAS  PubMed  Google Scholar 

  • Park S, Jung YT, Yoon JH (2013a) Pelagicola litorisediminis sp. nov., a novel alphaproteobacterium isolated from tidal flat sediment. Antonie Van Leeuwenhoek 104:103–110

    Article  CAS  PubMed  Google Scholar 

  • Park S, Jung YT, Lee JS, Lee KC, Yoon JH (2013b) Sabulilitoribacter multivorans gen. nov., sp. nov., a polysaccharide-degrading bacterium of the family Flavobacteriaceae isolated from seashore sand. Antonie Van Leeuwenhoek 104:973–981

    Article  CAS  PubMed  Google Scholar 

  • Park S, Lee JS, Lee KC, Yoon JH (2013c) Geojedonia litorea gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from coastal seawater. Antonie Van Leeuwenhoek 103:1255–1262

    Article  CAS  PubMed  Google Scholar 

  • Penesyan A, Breider S, Schumann P, Tindall BJ, Egan S, Brinkhoff T (2013) Epibacterium ulvae gen. nov., sp. nov., epibiotic bacteria isolated from the surface of a marine alga. Int J Syst Evol Microbiol 63:1589–1596

    Article  CAS  PubMed  Google Scholar 

  • Rüger HJ, Höfle MG (1992) Marine star-shaped-aggregate-forming bacteria: Agrobacterium atlanticum sp. nov.; Agrobacterium meteori sp. nov.; Agrobacterium ferrugineum sp. nov., nom. rev.; Agrobacterium gelatinovorum sp. nov., nom. rev.; and Agrobacterium stellulatum sp. nov., nom. rev. Int J Syst Bacteriol 42:133–143

    Article  PubMed  Google Scholar 

  • Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI technical note 101. Microbial ID, Inc., Newark

  • Stackebrandt E, Goebel BM (1994) Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849

    Article  CAS  Google Scholar 

  • Tamaoka J, Komagata K (1984) Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128

    Article  CAS  Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Uchino Y, Hirata A, Yokota A, Sugiyama J (1998) Reclassification of marine Agrobacterium species: proposals of Stappia stellulata gen. nov., comb. nov., Stappia aggregata sp. nov., nom. rev., Ruegeria atlantica gen. nov., comb. nov., Ruegeria gelatinovora comb. nov., Ruegeria algicola comb. nov., and Ahrensia kieliense gen. nov., sp. nov., nom. rev. J Gen Appl Microbiol 44:201–210

    Article  CAS  PubMed  Google Scholar 

  • Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE (1987) International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464

    Article  Google Scholar 

  • Yi H, Chun J (2006) Thalassobius aestuarii sp. nov., isolated from tidal flat sediment. J Microbiol 44:171–176

    CAS  PubMed  Google Scholar 

  • Yoon JH, Kim H, Kim SB, Kim HJ, Kim WY, Lee ST, Goodfellow M, Park YH (1996) Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505

    Article  CAS  Google Scholar 

  • Yoon JH, Lee ST, Park YH (1998) Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194

    Article  CAS  PubMed  Google Scholar 

  • Yoon JH, Kang KH, Park YH (2003) Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454

    Article  CAS  PubMed  Google Scholar 

  • Yoon JH, Kang SJ, Lee SY (2012) Salinimonas lutimaris sp. nov., a polysaccharide-degrading bacterium isolated from a tidal flat. Antonie Van Leeuwenhoek 102:803–810

    Article  Google Scholar 

  • Yoon JH, Gyeong HR, Kim SI (2013a) Sungkyunkwania multivorans gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from seawater from a seaweed farm. Int J Syst Evol Microbiol 63:1995–2001

    Article  CAS  PubMed  Google Scholar 

  • Yoon JH, Lee MH, Jung YT (2013b) Pseudofulvibacter geojedonensis gen. nov., sp. nov., a polysaccharide-degrading member of the family Flavobacteriaceae isolated from seawater, and emended description of the genus Fulvibacter. Int J Syst Evol Microbiol 63:1696–1701

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by the Program for Collection, Management and Utilization of Biological Resources (grant NRF-2013M3A9A5075953) from the Ministry of Science, ICT and Future Planning (MSIP) of the Republic of Korea.

Author information

Authors and Affiliations

  1. Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea

    Sooyeon Park, Yong-Taek Jung, Sung-Min Won, Ja-Min Park & Jung-Hoon Yoon

  2. University of Science and Technology (UST), 113 Gwahangno, Yuseong, Daejeon, South Korea

    Yong-Taek Jung

Authors
  1. Sooyeon Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yong-Taek Jung
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sung-Min Won
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ja-Min Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jung-Hoon Yoon
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jung-Hoon Yoon.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (PPT 145 kb)

Supplementary material 2 (PPT 236 kb)

Rights and permissions

Reprints and Permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Park, S., Jung, YT., Won, SM. et al. Thalassobius aquaeponti sp. nov., an alphaproteobacterium isolated from seawater. Antonie van Leeuwenhoek 106, 535–542 (2014). https://doi.org/10.1007/s10482-014-0225-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10482-014-0225-1

Keywords

search

Navigation