Antonie van Leeuwenhoek

, Volume 106, Issue 3, pp 457–463 | Cite as

Vibrio crosai sp. nov., isolated from a cultured oyster Crassostrea gigas

  • Adrián González-Castillo
  • Sabela Balboa
  • Jesús L. Romalde
  • Bruno Gomez-GilEmail author
Original Paper


A motile, facultative anaerobic, marine bacterial isolate (CAIM 1437T) was obtained from a cultured oyster (Crassostrea gigas) in Sonora, México. The strain was studied by a phylogenetic analysis based on sequences of the 16S rRNA and five housekeeping genes, i.e. ftsZ, gapA, pyrH, recA, and topA. Comparison of the almost-complete 16S rRNA gene sequence with those of other type strains of the genus Vibrio showed a close relationship with the type strains of Vibrio orientalis and Vibrio rotiferianus, with similarity values ranging from 98.4 to 98.3 %, respectively. MLSA placed this strain within the Orientalis clade. The DNA–DNA hybridization value of strain CAIM 1437T with V. orientalis was 59 % and with V. rotiferianus 55 %. The DNA G+C content was determined to be 45.6 mol %. Phenotypic characteristics also showed differences with the species analysed. The results presented here support the description of a novel species, for which the name Vibrio crosai sp. nov. is proposed, with CAIM 1437T (= DSM 27145T) as the type strain.


Vibrio crosai Vibrionaceae Orientalis clade 



Multilocus sequence analysis



This work was partially funded by CONACYT project CB-2009-01 132328 and grant AGL-2010-18438 from the Ministerio de Ciencia e Innovación (Spain). Thanks to Carmen Bolán for technical support.

Supplementary material

10482_2014_214_MOESM1_ESM.pdf (620 kb)
Supplementary material 1 (PDF 620 kb)


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Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • Adrián González-Castillo
    • 1
  • Sabela Balboa
    • 2
  • Jesús L. Romalde
    • 2
  • Bruno Gomez-Gil
    • 1
    Email author
  1. 1.CIADA.C. Mazatlán Unit for AquacultureMazatlánMexico
  2. 2.Departamento de Microbiología y Parasitología, CIBUSUniversidad de Santiago de CompostelaSantiago de CompostelaSpain

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