Advertisement

Antonie van Leeuwenhoek

, Volume 106, Issue 3, pp 457–463 | Cite as

Vibrio crosai sp. nov., isolated from a cultured oyster Crassostrea gigas

  • Adrián González-Castillo
  • Sabela Balboa
  • Jesús L. Romalde
  • Bruno Gomez-GilEmail author
Original Paper

Abstract

A motile, facultative anaerobic, marine bacterial isolate (CAIM 1437T) was obtained from a cultured oyster (Crassostrea gigas) in Sonora, México. The strain was studied by a phylogenetic analysis based on sequences of the 16S rRNA and five housekeeping genes, i.e. ftsZ, gapA, pyrH, recA, and topA. Comparison of the almost-complete 16S rRNA gene sequence with those of other type strains of the genus Vibrio showed a close relationship with the type strains of Vibrio orientalis and Vibrio rotiferianus, with similarity values ranging from 98.4 to 98.3 %, respectively. MLSA placed this strain within the Orientalis clade. The DNA–DNA hybridization value of strain CAIM 1437T with V. orientalis was 59 % and with V. rotiferianus 55 %. The DNA G+C content was determined to be 45.6 mol %. Phenotypic characteristics also showed differences with the species analysed. The results presented here support the description of a novel species, for which the name Vibrio crosai sp. nov. is proposed, with CAIM 1437T (= DSM 27145T) as the type strain.

Keywords

Vibrio crosai Vibrionaceae Orientalis clade 

Abbreviation

MLSA

Multilocus sequence analysis

Notes

Acknowledgments

This work was partially funded by CONACYT project CB-2009-01 132328 and grant AGL-2010-18438 from the Ministerio de Ciencia e Innovación (Spain). Thanks to Carmen Bolán for technical support.

Supplementary material

10482_2014_214_MOESM1_ESM.pdf (620 kb)
Supplementary material 1 (PDF 620 kb)

References

  1. Austin B, Austin DA (1999) Bacterial fish pathogens: disease of farmed and wild fish, 3rd edn. Springer-Verlag KG, BerlinGoogle Scholar
  2. Beaz-Hidalgo R, Balboa S, Romalde JL, Figueras MJ (2010) Diversity and pathogenicity of Vibrio species in cultured bivalve molluscs. Environ Microbiol Rep 2:34–43PubMedCrossRefGoogle Scholar
  3. Campbell MS, Wright AC (2003) Real-time PCR analysis of Vibrio vulnificus from oysters. Appl Environ Microbiol 69:7137–7144PubMedCrossRefPubMedCentralGoogle Scholar
  4. Cano-Gomez A, Goulden EF, Owens L, Høj L (2010) Vibrio owensii sp. nov., isolated from cultured crustaceans in Australia. FEMS Microbiol Lett 302:175–181PubMedCrossRefGoogle Scholar
  5. Cano-Gomez A, Høj L, Owens L, Andreakis N (2011) Multilocus sequence analysis provides basis for fast and reliable identification of Vibrio harveyi-related species and reveals previous misidentification of important marine pathogens. Syst Appl Microbiol 34:561–565PubMedCrossRefGoogle Scholar
  6. Chimetto LA, Cleenwerck I, Thompson CC, Brocchi M, Willems A, de Vos P, Thompson FL (2010) Photobacterium jeanii sp. nov., isolated from corals and zoanthids. Int J Syst Evol Microbiol 60:2843–2848PubMedCrossRefGoogle Scholar
  7. Colwell RR (1970) Polyphasic taxonomy of the genus vibrio: numerical taxonomy of Vibrio cholerae, Vibrio parahaemolyticus, and related Vibrio species. J Bacteriol 104(1):410–433PubMedPubMedCentralGoogle Scholar
  8. Gomez-Gil B, Roque A, Turnbull JF, Tron-Mayen L (1998) Species of Vibrio isolated from hepatopancreas, haemolymph and digestive tract of a population of healthy juvenile Penaeus vannamei. Aquaculture 163:1–9CrossRefGoogle Scholar
  9. Huson DH, Bryant D (2006) Application of phylogenetic networks in evolutionary studies. Mol Biol Evol 23(2):254–267PubMedCrossRefGoogle Scholar
  10. Jin C, Luo P, Zuo H, Chen J, Chen M, Wang W (2012) Vibrio zhanjiangensis sp. nov., isolated from sea water of shrimp farming pond. Antonie Van Leeuwenhoek 101(4):743–751PubMedCrossRefGoogle Scholar
  11. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721PubMedCrossRefGoogle Scholar
  12. MacFaddin JF (2000) Biochemical tests for identification of medical bacteria. Lippincott, Williams & Wilkins, LondonGoogle Scholar
  13. Macián MC, Ludwig W, Aznar R, Grimont P, Schleifer KH, Garay E, Pujalte MJ (2001) Vibrio lentus sp. nov., isolated from Mediterranean oysters. Int J Syst Evol Microbiol 51:1449–1456PubMedCrossRefGoogle Scholar
  14. Meier-Kolthoff JP, Göker M, Spröer C, Klenk H-P (2013) When should a DDH experiment be mandatory in microbial taxonomy? Arch Microbiol 195(6):413–418PubMedCrossRefGoogle Scholar
  15. Moreira AP, Pereira N Jr, Thompson FL (2011) Usefulness of a real-time PCR platform for G+C content and DNA–DNA hybridization estimations in vibrios. Int J Syst Evol Microbiol 61:2379–2383PubMedCrossRefGoogle Scholar
  16. Noguerola I, Blanch AR (2008) Identification of Vibrio spp. with a set of dichotomous keys. J Appl Microbiol 105:175–185PubMedCrossRefGoogle Scholar
  17. Pascual J, Macián MC, Arahal DR, Garay E, Pujalte MJ (2010) Multilocus sequence analysis of the central clade of the genus Vibrio by using the 16S rRNA, recA, pyrH, rpoD, gyrB, rctB and toxR genes. Int J Syst Evol Microbiol 60:154–165PubMedCrossRefGoogle Scholar
  18. Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. Microbial ID Inc, NewarkGoogle Scholar
  19. Sawabe T, Kita-Tsukamoto K, Thompson FL (2007) Inferring the evolutionary history of vibrios by means of multilocus sequence analysis. J Bacteriol 189:7932–7936PubMedCrossRefPubMedCentralGoogle Scholar
  20. Stackebrandt E, Ebers J (2006) Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155Google Scholar
  21. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739PubMedCrossRefPubMedCentralGoogle Scholar
  22. Thompson FL, Swings J (2006) Taxonomy of the vibrios. In: Thompson FL, Austin B, Swings J (eds) The biology of vibrios. American Society for Microbiology, Washington, DC, pp 29–43CrossRefGoogle Scholar
  23. Thompson FL, Li Y, Gomez-Gil B, Thompson CC, Hoste B, Vandemeulebroecke K, Rupp GS, Pereira A, De Bem MM, Sorgeloos P, Swings J (2003) Vibrio neptunius sp. nov., Vibrio brasiliensis sp. nov. and Vibrio xuii sp. nov., isolated from the marine aquaculture environment (bivalves, fish, rotifers and shrimps). Int J Syst Evol Microbiol 53:245–252PubMedCrossRefGoogle Scholar
  24. Wang Y, Zhang X-H, Min Y, Wang H, Austin B (2010) Vibrio atypicus sp. nov., isolated from the digestive tract of the Chinese prawn (Penaeus chinensis O`sbeck). Int J Syst Evol Microbiol 60:2517–2523PubMedCrossRefGoogle Scholar
  25. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky L, Moore LH, Murray RGE, Stackenbrandt E, Starr MP, Truper HG (1987) Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464CrossRefGoogle Scholar
  26. Yoshizawa S, Wada M, Yokota A, Kogure K (2010) Vibrio sagamiensis sp. nov., luminous marine bacteria isolated from sea water. J Gen Appl Microbiol 56:499–507PubMedCrossRefGoogle Scholar
  27. Ziemke F, Höfle MG, Lalucat J, Rosselló-Mora R (1998) Reclassification of Shewanella putrefaciens Owen’s genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48:179–186PubMedCrossRefGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • Adrián González-Castillo
    • 1
  • Sabela Balboa
    • 2
  • Jesús L. Romalde
    • 2
  • Bruno Gomez-Gil
    • 1
    Email author
  1. 1.CIADA.C. Mazatlán Unit for AquacultureMazatlánMexico
  2. 2.Departamento de Microbiología y Parasitología, CIBUSUniversidad de Santiago de CompostelaSantiago de CompostelaSpain

Personalised recommendations