Flavimarina pacifica gen. nov., sp. nov., a new marine bacterium of the family Flavobacteriaceae, and emended descriptions of the genus Leeuwenhoekiella, Leeuwenhoekiella aequorea and Leeuwenhoekiella marinoflava


A facultatively anaerobic, Gram-stain negative, rod-shaped and yellow pigmented bacterium, designated strain IDSW-73T, was isolated from a seawater sample and subjected to a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the novel strain formed a distinct phyletic line in the family Flavobacteriaceae and is most closely related to the members of the genus Leeuwenhoekiella, with 16S rRNA gene sequence similarity of 91.4–92.6 %. Strain IDSW-73T was found to be able to grow with 0–12 % NaCl and at 4–33 °C; and was able to hydrolyse gelatin, starch and Tweens 20, 40 and 80. The DNA G+C content was determined to be 42.2 mol%. The predominant cellular fatty acids were identified as branched-chain saturated and unsaturated and straight-chain unsaturated fatty acids such as iso-C15:0, iso-C15:1, iso-C17:1 ω9c, C15:1 ω6c, iso-C15:0 3-OH, iso-C17:0 3-OH and summed feature 3 (as defined by MIDI), comprising iso-C15:0 2-OH and/or C16:1 ω7c. The polar lipids found were phosphatidylethanolamine, two unknown aminolipids and one unknown lipid. The major respiratory quinone was identified as MK-6. The significant molecular distinctiveness between the novel isolate and its nearest neighbours were strongly supported by notable differences in physiological and biochemical tests. Therefore, strain IDSW-73T is considered to represent a novel genus and species within the family Flavobacteriaceae, for which the name Flavimarina pacifica gen. nov., sp. nov. is proposed. The type strain is IDSW-73T (=KCTC 32466T = KMM 6759T). Emended descriptions of the recognized species of the genus Leeuwenhoekiella are also proposed.

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  1. Alonso C, Warnecke F, Amann R, Pernthaler J (2007) High local and global diversity of Flavobacteria in marine plankton. Environ Microbiol 9:1253–1266

    CAS  PubMed  Article  Google Scholar 

  2. Bernardet JF, Nakagawa Y, Holmes B (2002) Proposal minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. IntJ Syst Evol Microbiol 52:1049–1070

    CAS  Article  Google Scholar 

  3. Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917

    CAS  PubMed  Article  Google Scholar 

  4. Collins MD, Shah HM (1984) Fatty acid, menaquinone and polar lipid composition of Rothia dentosacariosa. Arch Microbiol 137:247–249

    CAS  Article  Google Scholar 

  5. Dang H, Li T, Chen M, Huang G (2008) Cross-ocean distribution of Rhodobacteriales bacteria as primary surface colonizers in temperate coastal marine waters. Appl Environ Microbiol 74:52–60

    CAS  PubMed Central  PubMed  Article  Google Scholar 

  6. Diez-Vivas C, Gasol JM, Acinas SJ (2014) Spatial and temporal variability among marine Bacteroidetes populations in the NW Mediterranean Sea. Syst Appl Microbiol 37:68–78

    Article  Google Scholar 

  7. Felsenstein J (1993) PHYLIP (phylogenetic inference package), version 3.5c. Department of Genetic, University of Washington, Seattle, USA

  8. Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) (1994) Methods for general and molecular bacteriology. American Society for Microbiology, Washington

    Google Scholar 

  9. Hahnke RL, Harder J (2013) Phylogenetic diversity of Flavobacteria isolated from the North Sea on solid media. Syst Appl Microbiol 36:497–504

    CAS  PubMed  Article  Google Scholar 

  10. Jukes TH, Cantor CR (1969) Evolution of protein molecules. In: Munro HN (ed) Mammalian protein metabolism, vol 3. Academic Press, New York, pp 21–213

    Chapter  Google Scholar 

  11. Komagata K, Suzuki K-I (1987) Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19:161–207

    CAS  Article  Google Scholar 

  12. Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, Chichester, pp 115–175

    Google Scholar 

  13. Lemos ML, Toranzo AE, Barja JL (1985) Modified medium for oxidation-fermentation test in the identification of marine bacteria. Appl Environ Microbiol 40:1541–1543

    Google Scholar 

  14. Marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218

    CAS  Article  Google Scholar 

  15. Marmur J, Doty P (1962) Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118

    CAS  PubMed  Article  Google Scholar 

  16. Nedashkovskaya OI, Vancanneyt M, Dawyndt P, Engelbeen K, Vandemeulebroecke K, Cleenwerck I, Hoste B, Mergaert J, Tan TL, Frolova GM, Mikhailov VV, Swings J (2005) Reclassification of [Cytophaga] marinoflava Reichenbach 1989 as Leeuwenhoekiella marinoflava gen. nov., comb. nov. and description of Leeuwenhoekiella aequorea sp. nov. Int J Syst Evol Microbiol 55:1033–1038

    CAS  PubMed  Article  Google Scholar 

  17. Nedashkovskaya OI, Vancanneyt M, Kim SB, Zhukova NV, Han JH, Mikhailov VV (2009) Leeuwenhoekiella palythoae sp. nov., a new member of the family Flavobacteriaceae. Int J Syst Evol Microbiol 59:3074–3077

    CAS  PubMed  Article  Google Scholar 

  18. Pinhassi J, Bowman JP, Nedashkovskaya OI, Lekunberri I, Gomez-Comsarnau L, Pedrós-Alió C (2006) Leeuwenhoekiella blandensis sp. nov., a genome-sequenced marine member of the family Flavobacteriaceae. Int J Syst Evol Microbiol 56:1489–1493

    CAS  PubMed  Article  Google Scholar 

  19. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  20. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor

    Google Scholar 

  21. Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:16

    Google Scholar 

  22. Simonato F, Goméz-Pereira PR, Fuchs BM, Amann R (2010) Bacterioplankton diversity and community composition in the Southern Lagoon in Venice. Syst Appl Microbiol 33:128–138

    CAS  PubMed  Article  Google Scholar 

  23. Vieira RP, Gonzalez AM, Cardoso AM, Oliveira DN, Albano RM, Clementino MM, Martins OB, Paranhos R (2008) Relationships between bacterial diversity and environmental variables in a tropical marine environment, Rio de Janeiro. Environ Microbiol 10:189–199

    CAS  PubMed  Google Scholar 

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This research was supported by grants of the Presidium of the Russian Academy of Sciences “Molecular and Cell Biology”, Russian Scientific Fund no. 14-14-00030, the Presidium of the Far-Eastern Branch of the Russian Academy of Sciences no. 12-III-A-06-105 and the government of Russian Federation for the state support of scientific investigations conducting under the guidance of the leading researchers at the Russian education institutions of the high professional education, agreement no. 11.G34.31.0010.

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Correspondence to Olga I. Nedashkovskaya.

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Nedashkovskaya, O.I., Kukhlevskiy, A.D., Zhukova, N.V. et al. Flavimarina pacifica gen. nov., sp. nov., a new marine bacterium of the family Flavobacteriaceae, and emended descriptions of the genus Leeuwenhoekiella, Leeuwenhoekiella aequorea and Leeuwenhoekiella marinoflava . Antonie van Leeuwenhoek 106, 421–429 (2014). https://doi.org/10.1007/s10482-014-0210-8

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  • Flavimarina pacifica gen. nov., sp. nov.
  • Flavobacteriaceae
  • Marine bacteria
  • Phylogeny
  • Taxonomy