Flavimarina pacifica gen. nov., sp. nov., a new marine bacterium of the family Flavobacteriaceae, and emended descriptions of the genus Leeuwenhoekiella, Leeuwenhoekiella aequorea and Leeuwenhoekiella marinoflava

Abstract

A facultatively anaerobic, Gram-stain negative, rod-shaped and yellow pigmented bacterium, designated strain IDSW-73T, was isolated from a seawater sample and subjected to a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the novel strain formed a distinct phyletic line in the family Flavobacteriaceae and is most closely related to the members of the genus Leeuwenhoekiella, with 16S rRNA gene sequence similarity of 91.4–92.6 %. Strain IDSW-73T was found to be able to grow with 0–12 % NaCl and at 4–33 °C; and was able to hydrolyse gelatin, starch and Tweens 20, 40 and 80. The DNA G+C content was determined to be 42.2 mol%. The predominant cellular fatty acids were identified as branched-chain saturated and unsaturated and straight-chain unsaturated fatty acids such as iso-C15:0, iso-C15:1, iso-C17:1 ω9c, C15:1 ω6c, iso-C15:0 3-OH, iso-C17:0 3-OH and summed feature 3 (as defined by MIDI), comprising iso-C15:0 2-OH and/or C16:1 ω7c. The polar lipids found were phosphatidylethanolamine, two unknown aminolipids and one unknown lipid. The major respiratory quinone was identified as MK-6. The significant molecular distinctiveness between the novel isolate and its nearest neighbours were strongly supported by notable differences in physiological and biochemical tests. Therefore, strain IDSW-73T is considered to represent a novel genus and species within the family Flavobacteriaceae, for which the name Flavimarina pacifica gen. nov., sp. nov. is proposed. The type strain is IDSW-73T (=KCTC 32466T = KMM 6759T). Emended descriptions of the recognized species of the genus Leeuwenhoekiella are also proposed.

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Acknowledgments

This research was supported by grants of the Presidium of the Russian Academy of Sciences “Molecular and Cell Biology”, Russian Scientific Fund no. 14-14-00030, the Presidium of the Far-Eastern Branch of the Russian Academy of Sciences no. 12-III-A-06-105 and the government of Russian Federation for the state support of scientific investigations conducting under the guidance of the leading researchers at the Russian education institutions of the high professional education, agreement no. 11.G34.31.0010.

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Correspondence to Olga I. Nedashkovskaya.

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Nedashkovskaya, O.I., Kukhlevskiy, A.D., Zhukova, N.V. et al. Flavimarina pacifica gen. nov., sp. nov., a new marine bacterium of the family Flavobacteriaceae, and emended descriptions of the genus Leeuwenhoekiella, Leeuwenhoekiella aequorea and Leeuwenhoekiella marinoflava . Antonie van Leeuwenhoek 106, 421–429 (2014). https://doi.org/10.1007/s10482-014-0210-8

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Keywords

  • Flavimarina pacifica gen. nov., sp. nov.
  • Flavobacteriaceae
  • Marine bacteria
  • Phylogeny
  • Taxonomy