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Bacillus huizhouensis sp. nov., isolated from a paddy field soil

Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

A Gram-stain positive, facultative aerobic bacterium, designated as strain GSS03T, was isolated from a paddy field soil. The cells were observed to be endospore forming, rod-shaped and motile with flagella. The organism was found to grow optimally at 35 °C at pH 7.0 and in the presence of 1 % NaCl. The strain was classified as a novel taxon within the genus Bacillus on the basis of phenotypic and phylogenetic analyses. The closest phylogenetic relatives were identified as Bacillus psychrosaccharolyticus DSM 6T (97.61 %), Bacillus muralis DSM 16288T (97.55 %), Bacillus asahii JCM 12112T (97.48 %), Bacillus simplex DSM 1321T (97.48 %) and “Bacillus frigoritolerans” DSM 8801T (97.38 %). The menaquinone was identified as MK-7, the major cellular fatty acid was identified as anteiso-C15:0 and the major cellular polar lipids as phosphatidylethanolamine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and three unknown polar lipids. The DNA G+C content was determined to be 40.2 mol%. The DNA–DNA relatedness with the closest relatives was below 48 %. Therefore, on the basis of all the results, strain GSS03T is considered to represent a novel species within the genus Bacillus, for which the name Bacillus huizhouensis sp. nov. is proposed. The type strain is GSS03T (=KCTC 33172T =CCTCC AB 2013237T).

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Acknowledgments

This work was supported by the Team Project of Guangdong Natural Science Foundation (S2011030002882 and S2012030006114).

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Authors and Affiliations

  1. College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, Peoples’s Republic of China

    Jibing Li & Yong Zhao

  2. Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou, 510650, Peoples’s Republic of China

    Jibing Li, Guiqin Yang, Min Wu & Shungui Zhou

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  1. Jibing Li
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  2. Guiqin Yang
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Correspondence to Shungui Zhou.

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Li, J., Yang, G., Wu, M. et al. Bacillus huizhouensis sp. nov., isolated from a paddy field soil. Antonie van Leeuwenhoek 106, 357–363 (2014). https://doi.org/10.1007/s10482-014-0208-2

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