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Aquimarina atlantica sp. nov., isolated from surface seawater of the Atlantic Ocean

Antonie van Leeuwenhoek volume 106, pages 293–300 (2014)Cite this article

Abstract

A taxonomic study was carried out on strain 22II-S11-z7T, which was isolated from the surface seawater of the Atlantic Ocean. The bacterium was found to be Gram-negative, oxidase negative and catalase positive, long-rod shaped, and gliding. Growth was observed at salinities of 1–5 % and at temperatures of 10–41 °C. The isolate was capable of hydrolysing gelatin and Tween 80 and able to reduce nitrate to nitrite, but unable to degrade aesculin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22II-S11-z7T belongs to the genus Aquimarina, with highest sequence similarity to Aquimarina megaterium XH134T (98.31 %), followed by Aquimarina macrocephali JAMB N27T (96.59 %); other species of the genus Aquimarina shared 93.63–96.08 % sequence similarity. The ANI value between strain 22II-S11-z7T and A. megaterium XH134T was found to be 91.86–91.81 %. The DNA–DNA hybridization estimated value between strain 22II-S11-z7T and A. megaterium XH134T was 47.7 ± 2.6 %. The principal fatty acids were identified as Summed Feature 3 (C16:1 ω7c/ω6c, as defined by the MIDI system; 8.1 %), SummedFeature 9 (iso-C17:1 ω7c/C16:110-methyl; 6.8 %), iso-C15:0 G (11.3 %), iso-C15:0 (24.9 %), iso-C16:0 (5.7 %), C16:0 (5.2 %), iso-C15:0 3OH (6.4 %) and iso-C17:0 3OH (21.5 %). The G+C content of the chromosomal DNA was determined to be 32.99 mol %. The respiratory quinone was determined to be MK-6 (100 %). Phosphatidylethanolamine, two unidentified aminolipids, five unidentified phospholipids and two unidentified lipids were found to be present. The combined genotypic and phenotypic data show that strain 22II-S11-z7T represents a novel species within the genus Aquimarina, for which the name Aquimarina atlantica sp. nov. is proposed, with the type strain 22II-S11-z7T (=MCCC 1A09239T = KCTC 42003T).

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Fig. 1

Abbreviations

MCCC:

Marine Culture Collection of China

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Acknowledgments

This work was financially supported by COMRA program (No. DY125-15-R-01), Public Welfare Project of SOA (201005032) and National Infrastructure of Natural Resources for Science and Technology Program of China (No. NIMR-2013-9). We thank Professor Zhang X.H. for generous gift of A. megaterium XH134T.

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Authors and Affiliations

  1. State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Collaborative Innovation Center of Deep Sea Biology; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen, 361005, China

    Guizhen Li, Qiliang Lai, Fengqin Sun, Xiupian Liu, Yunbiao Xie, Yaping Du, Guangyu Li & Zongze Shao

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  1. Guizhen Li
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  2. Qiliang Lai
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  7. Guangyu Li
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Correspondence to Zongze Shao.

Additional information

Guizhen Li and Qiliang Lai are contributed equally to this work.

The GenBank accession number for the 16S rRNA gene sequence of Aquimarina atlantica 22II-S11-z7T is KF906719. Transmission electron micrographs of cells of strain 22II-S11-z7T, polar lipids of strain 22II-S11-z7T, Maximum-Likelihood tree and Minimum Evolution tree are available as supplementary figures in ANTO Online.

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Li, G., Lai, Q., Sun, F. et al. Aquimarina atlantica sp. nov., isolated from surface seawater of the Atlantic Ocean. Antonie van Leeuwenhoek 106, 293–300 (2014). https://doi.org/10.1007/s10482-014-0196-2

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Keywords

  • Aquimarina atlantica sp. nov.
  • Atlantic Ocean
  • Taxonomy
  • Surface seawater