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Halomonas qiaohouensis sp. nov., isolated from salt mine soil in southwest China

Antonie van Leeuwenhoek volume 106, pages 253–260 (2014)Cite this article

Abstract

Two Gram-stain negative, moderately halophilic, aerobic, motile bacteria, designated strains YIM QH88T and YIM QH103, were isolated from the Qiaohou salt mine in Yunnan, southwest China. Cells of the strains were observed to be rod-shaped and produce creamy-coloured colonies. Growth of the two strains was observed at 10–45 °C (optimum 25–37 °C), at pH 6.0–10.0 (optimum 7.0–8.0), and in the presence of 0.5–20 % (w/v) NaCl (optimum 2–6 %). The two strains were found to contain summed feature 8 (C18:1 ω7c/ω6c), C19:0 cyclo ω8c and C16:0 as the major cellular fatty acids. The polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and unknown phospholipid. The G+C content of the genomic DNA of strains YIM QH88T and YIM QH103 were determined to be 64.6 and 64.2 mol%, respectively, and the predominant respiratory quinone detected was ubiquinone 9. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains YIM QH88T and YIM QH103 formed a distinct lineage within the genus Halomonas and were most closely related to Halomonas pantelleriensis DSM 9661T with 97.3 and 97.5 % of 16S rRNA sequence similarity respectively. The DNA–DNA hybridization relatedness value for strains YIM QH88T and YIM QH103 was 95.2 ± 0.8 %. The levels of DNA–DNA relatedness between each of these two strains and the type strains of phylogenetically closely related Halomonas species were clearly below 70 %. On the basis of their phylogenetic analysis, DNA–DNA hybridization relatedness, phenotypic and chemotaxonomic characteristics, strains YIM QH88T and YIM QH103 should be classified as a novel species of the genus Halomonas, for which the name Halomonas qiaohouensis sp. nov. is proposed. The type strain is YIM QH88T (=DSM 26770T =CCTCC AB 2012965T).

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References

  • Arahal DR, Ventosa A (2006) The family Halomonadaceae. In: Dworkin M, Falkow S, Rosenberg E, Schleifer K-H, Stackebrandt E (eds) The prokaryotes: a handbook on the biology of bacteria, vol 6, 3rd edn. Springer, New York, pp 811–835

    Chapter  Google Scholar 

  • Arahal DR, Vreeland RH, Litchfield CD, Mormile MR, Tindall BJ, Oren A, Béjar V, Quesada E, Ventosa A (2007) Recommended minimal standards for describing new taxa of the family Halomonadaceae. Int J Syst Evol Microbiol 57:2436–2446

    CAS  PubMed  Article  Google Scholar 

  • Arenas M, Banon PI, Copa-Patino JL, Sánchez-Porro C, Ventosa A, Soliveri J (2009) Halomonas ilicicola sp. nov., a moderately halophilic bacterium isolated from a saltern. Int J Syst Evol Microbiol 59:578–582

    CAS  PubMed  Article  Google Scholar 

  • Arias S, del Moral A, Ferrer MR, Tallon R, Quesada E, Béjar V (2003) Mauran, an exopolysaccharide produced by the halophilic bacterium Halomonas maura, with a novel composition and interesting properties for biotechnology. Extremophiles 7:319–326

    CAS  PubMed  Article  Google Scholar 

  • Ashelford KE, Chuzhanova NA, Fry JC, Jones AJ, Weightman AJ (2006) New screening software shows that most recent large 16S rRNA gene clone libraries contain chimeras. Appl Environ Microbiol 72:5734–5741

    CAS  PubMed Central  PubMed  Article  Google Scholar 

  • Bauer AW, Kirby WM, Sherris JC, Turck M (1966) Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45:493–496

    CAS  PubMed  Google Scholar 

  • Cho JC, Giovannoni SJ (2006) Pelagibaca bermudensis gen. nov., sp. nov., a novel marine bacterium within the Roseobacter clade in the order Rhodobacterales. Int J Syst Evol Microbiol 56:855–859

    CAS  PubMed  Article  Google Scholar 

  • Clarke PH (1953) Hydrogen sulphide production by bacteria. J Gen Microbiol 8:397–407

    CAS  PubMed  Article  Google Scholar 

  • Collins MD, Pirouz T, Goodfellow M, Minnikin DE (1977) Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230

    CAS  PubMed  Article  Google Scholar 

  • Cui XL, Mao PH, Zeng M, Li WJ, Zhang LP, Xu LH, Jiang CL (2001) Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51:357–363

    CAS  PubMed  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Franzmann PD, Wehmeyer U, Stackebrandt E (1988) Halomonadaceae fam. nov., a new family of the class Proteobacteria to accommodate the genera Halomonas and Deleya. Syst Appl Microbiol 11:16–19

    Article  Google Scholar 

  • Guan TW, Xiao J, Zhao K, Luo XX, Zhang XP, Zhang LL (2010) Halomonas xinjiangensis sp. nov., a halotolerant bacterium isolated from a salt lake. Int J Syst Evol Microbiol 60:349–752

    CAS  PubMed  Article  Google Scholar 

  • Guzmán D, Quillaguamán J, Munoz M, Hatti-Kaul R (2010) Halomonas andesensis sp. nov., a moderate halophile isolated from the saline lake Laguna Colorada in Bolivia. Int J Syst Evol Microbiol 60:749–753

    PubMed  Article  Google Scholar 

  • Høvik Hansen GH, Sørheim R (1991) Improved method for phenotypical characterization of marine bacteria. J Microbiol Methods 13:231–241

    Article  Google Scholar 

  • Kaye JZ, Márquez MC, Ventosa A, Baross JA (2004) Halomonas neptunia sp. nov., Halomonas sulfidaeris sp. nov., Halomonas axialensis sp. nov. and Halomonas hydrothermalis sp. nov.: halophilic bacteria from deep-sea hydrothermal-vent environments. Int J Syst Bacteriol 54:499–511

    CAS  Article  Google Scholar 

  • Kim MS, Roh SW, Bae JW (2011) Halomonas jeotgali sp. nov., a new moderate halophilic bacterium isolated from a traditional fermented seafood. J Microbiol 48:404–410

    Article  Google Scholar 

  • Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721

    CAS  PubMed  Article  Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    CAS  PubMed  Article  Google Scholar 

  • Kovacs N (1956) Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178:703–704

    CAS  PubMed  Article  Google Scholar 

  • Lim JM, Yoon JH, Lee JC, Jeon CO, Park DJ, Sung C, Kim CJ (2004) Halomonas koreensis sp. nov., a novel moderately halophilic bacterium isolated from a solar saltern in Korea. Int J Syst Evol Microbiol 54:2037–2042

    CAS  PubMed  Article  Google Scholar 

  • Margesin R, Schinner F (2001) Potential of halotolerant and halophilic microorganisms for biotechnology. Extremophiles 5:73–83

    CAS  PubMed  Article  Google Scholar 

  • Martínez-Cánovas MJ, Quesada E, Llamas I, Béjar V (2004a) Halomonas ventosae sp. nov., a moderately halophilic, denitrifying, exopolysaccharide producing bacterium. Int J Syst Evol Microbiol 54:733–737

    PubMed  Article  Google Scholar 

  • Martínez-Cánovas MJ, Béjar V, Martínez-Checa F, Quesada E (2004b) Halomonas anticariensis sp. nov., from Fuente de Piedra, a saline-wetland wildfowl reserve in Málaga, southern Spain. Int J Syst Evol Microbiol 54:1329–1332

    PubMed  Article  Google Scholar 

  • Mata JA, Martínez-Cánovas J, Quesada E, Béjar V (2002) A detailed phenotypic characterisation of the type strains of Halomonas species. Syst Appl Microbiol 25:360–375

    CAS  PubMed  Article  Google Scholar 

  • Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39:159–167

  • Minnikin DE, Collins MD, Goodfellow M (1979) Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47:87–95

    CAS  Article  Google Scholar 

  • Poli A, Schiano Moriello V, Esposito E, Lama L, Gambacorta A, Nicolaus B (2004) Exopolysaccharide production by a new Halomonas strain CRSS isolated from saline lake Cape Russell in Antarctica growing on complex and defined media. Biotechnol Lett 26:1635–1638

    CAS  PubMed  Article  Google Scholar 

  • Quesada E, Béjar V, Ferrer MR, Calvo C, Llamas I, Martìnez-Checa F, Arias S, Ruiz-García C, Páez R et al (2004) Moderately halophilic, exopolysaccharide-producing bacteria. In: Ventosa A (ed) Halophilic microorganisms. Springer, Heildelberg, pp 297–314

    Chapter  Google Scholar 

  • Reddy GSN, Raghavan PUM, Sarita NB, Prakash JSS, Nagesh N, Delie D, Shivaji S (2003) Halomonas glaciei sp. nov. isolated from fast ice of Adelie Land Antarctica. Extremophiles 7:55–61

    CAS  PubMed  Google Scholar 

  • Romanenko LA, Schumann P, Rohde M, Mikhailov VV, Stackebrandt E (2002) Halomonas halocynthiae sp. nov., isolated from the marine ascidian Halocynthia aurantium. Int J Syst Evol Microbiol 52:1767–1772

    CAS  PubMed  Article  Google Scholar 

  • Romano I, Nicolaus B, Lama L, Manca MC, Gambacorta A (1996) Characterization of a haloalkalophilic strictly aerobic bacterium, isolated from Pantelleria island. Syst Appl Microbiol 19:326–333

    Article  Google Scholar 

  • Romano I, Giordano A, Lama L, Nicolaus B, Gambacorta A (2005) Halomonas campaniensis sp. nov., a haloalkaliphilic bacterium isolated from a mineral pool of Campania Region. Italy. Syst Appl Microbiol 28:610–618

    CAS  Article  Google Scholar 

  • Sánchez-Porro C, Martín S, Mellado E, Ventosa A (2003) Diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes. J Appl Microbiol 94:295–300

    PubMed  Article  Google Scholar 

  • Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:16

    Google Scholar 

  • Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular microbiology. American Society for Microbiology, Washington DC, pp 611–654

    Google Scholar 

  • Stackebrandt E, Goebel BM (1994) Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849

  • Suzuki M, Nakagawa Y, Harayama S, Yamamoto S (2001) Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 51:1639–1652

    CAS  PubMed  Article  Google Scholar 

  • Tamaoka J, Katayama-Fujimura Y, Kuraishi H (1983) Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 54:31–36

    CAS  Article  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA 5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    CAS  PubMed Central  PubMed  Article  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    CAS  PubMed Central  PubMed  Article  Google Scholar 

  • Tindall BJ (1990a) A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130

    CAS  Article  Google Scholar 

  • Tindall BJ (1990b) Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66:199–202

    CAS  Article  Google Scholar 

  • Ventosa A, Nieto JJ (1995) Biotechnological applications and potentialities of halophilic microorganisms. World J Microbiol Biotechnol 11:85–94

    CAS  PubMed  Article  Google Scholar 

  • Ventosa A, Nieto JJ, Oren A (1998) Biology of moderately halophilic aerobic bacteria. Microbiol Mol Biol Rev 62:504–544

    CAS  PubMed Central  PubMed  Google Scholar 

  • Vreeland RH, Litchfield CD, Martin EL, Elliot E (1980) Halomonas elongata, a new genus and species of extremely salt-tolerant bacteria. Int J Syst Bacteriol 30:485–495

    CAS  Article  Google Scholar 

  • Wang Y, Tang SK, Lou K, Mao PH, Jin X, Jiang CL, Xu LH, Li WJ (2008) Halomonas lutea sp. nov., a moderately halophilic bacterium isolated from a salt lake. Int J Syst Evol Microbiol 58:2065–2069

    CAS  PubMed  Article  Google Scholar 

  • Wang CY, Wu SJ, Ng CC, Tzeng WS, Shyu YT (2012) Halomonas beimenensis sp. nov., isolated from Beimen, an abandoned saltern in southern Taiwan. Int J Syst Bacteriol 62:3013–3017

    CAS  Article  Google Scholar 

  • Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ, Chen HH, Xu LH, Jiang CL (2005) Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55:1149–1153

    CAS  PubMed  Article  Google Scholar 

  • Yoon JH, Lee KC, Kho YH, Kang KH, Kim CJ, Park YH (2002) Halomonas alimentaria sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 52:123–130

    CAS  PubMed  Article  Google Scholar 

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (NSFC) (31200138, 31160123 and 31000003), the Ministry of Environmental Protection of China (National Key Sciences and Technology Program for Water Solutions, grant 2012ZX07102-003), the Yunnan Provincial Sciences and Technology Department (2009DA002), and the Life Science College of Yunnan University (2013S202). We are grateful to Mr Wei Chen and Mr Ming Wu for their help during sampling.

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The authors declare that they have no conflict of interest.

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  1. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, Yunnan, People’s Republic of China

    Yong-Xia Wang, Wei Xiao, Ming-Hua Dong, Qin Zhao, Zhi-Ying Li, Yong-Hong Lai & Xiao-Long Cui

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  1. Yong-Xia Wang
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  2. Wei Xiao
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Correspondence to Xiao-Long Cui.

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Yong-Xia Wang and Wei Xiao contributed equally to this work.

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Wang, YX., Xiao, W., Dong, MH. et al. Halomonas qiaohouensis sp. nov., isolated from salt mine soil in southwest China. Antonie van Leeuwenhoek 106, 253–260 (2014). https://doi.org/10.1007/s10482-014-0189-1

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Keywords

  • Halomonas qiaohouensis
  • Novel species
  • Halophilic bacteria
  • Salt mine