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Anhydrobiosis in yeast: is it possible to reach anhydrobiosis for yeast grown in conditions with severe oxygen limitation?

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Abstract

The yeast Saccharomyces cerevisiae was shown to be extremely sensitive to dehydration–rehydration treatments when stationary phase cells were subjected to conditions of severe oxygen limitation, unlike the same cells grown in aerobic conditions. The viability of dehydrated anaerobically grown yeast cells never exceeded 2 %. It was not possible to increase this viability using gradual rehydration of dry cells in water vapour, which usually strongly reduces damage to intracellular membranes. Specific pre-dehydration treatments significantly increased the resistance of anaerobic yeast to drying. Thus, incubation of cells with trehalose (100 mM), increased the viability of dehydrated cells after slow rehydration in water vapour to 30 %. Similarly, pre-incubation of cells in 1 M xylitol or glycerol enabled up to 50–60 % of cells to successfully enter a viable state of anhydrobiosis after subsequent rehydration. We presume that trehalose and sugar alcohols function mainly according to a water replacement hypothesis, as well as initiating various protective intracellular reactions.

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Abbreviations

Tm :

Phase transition temperature

APL:

Area per lipid

NADH:

Reduced form of nicotinamide adenine dinucleotide

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Acknowledgments

This research was financially supported by the European Regional Development Fund Project (No. 2010/0288/2DP/2.1.1.1.0/10/APIA/VIAA/038) and the Latvian Science Council Project No. 372/2012.

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Authors and Affiliations

  1. Laboratory of Cell Biology, Institute of Microbiology and Biotechnology, University of Latvia, Kronvald Blvd., 4, Riga, 1586, Latvia

    Linda Rozenfelde & Alexander Rapoport

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  1. Linda Rozenfelde
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  2. Alexander Rapoport
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Correspondence to Linda Rozenfelde.

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Rozenfelde, L., Rapoport, A. Anhydrobiosis in yeast: is it possible to reach anhydrobiosis for yeast grown in conditions with severe oxygen limitation?. Antonie van Leeuwenhoek 106, 211–217 (2014). https://doi.org/10.1007/s10482-014-0182-8

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  • DOI: https://doi.org/10.1007/s10482-014-0182-8

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