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Aquamicrobium terrae sp. nov., isolated from the polluted soil near a chemical factory

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Abstract

A Gram-negative, aerobic, non-motile bacterial strain hun6T isolated from the polluted soil near a chemical factory in northern Nanjing, China was investigated to clarify its taxonomic position. Growth of strain hun6T occurred between 10 and 45 °C (optimum, 30 °C) and between pH 6.0 and 8.0 (optimum, pH 7.0). No growth occurred at NaCl concentrations greater than 5 % (w/v). The 16S rRNA gene sequence analysis indicated that strain hun6T belongs to the genus Aquamicrobium. The sequence similarities of strain hun6T to other type strains of Aquamicrobium genus were all below 98.5 %. The presence of ubiquinone-10, the predominant fatty acid summed feature 8 (C18:1 ω7c and/or C18:1 ω6c) and C19:0 cyclo ω8c, a polar lipid pattern with phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, phosphatidylethanolamine and phophatidylmonomethylethanoamine were in accord with the characteristics of the genus Aquamicrobium. The G+C content of the genomic DNA was determined to be 63.5 mol%. The results of DNA–DNA hybridization, physiological and biochemical tests and chemotaxonomic properties allowed genotypic and phenotypic differentiation of strain hun6T from all known Aquamicrobium species. Therefore, strain hun6T can be assigned to a new species of this genus for which the name Aquamicrobium terrae sp. nov. is proposed. The type strain is hun6T (= CICC 10733T = DSM 27865T).

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Acknowledgments

This work was financially supported by the National Key Basic Research Program of China (No. 2014CB441105), the National Natural Science Foundation of China (41030531, 21277148), the Natural Science Foundation of Tianjin (No. 08JCZDJC15600), the Postdoctoral Special Support of China (No.2013T60568) and the National Postdoctoral Foundation of China (No. 2013M541745).

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Authors and Affiliations

  1. State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, People’s Republic of China

    Zhi-Guo Wu, Fang Wang, Cheng-Gang Gu, Yin-Ping Zhang & Xin Jiang

  2. Tianjin Key Laboratory of Pulp & Paper, Tianjin University of Science & Technology, Tianjin, 300457, People’s Republic of China

    Zhi-Guo Wu & Zong-Zheng Yang

  3. E-TECH Energy Technology Development Co.,Ltd, Tianjin, 300384, People’s Republic of China

    Xiao-Wei Wu

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  1. Zhi-Guo Wu
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  2. Fang Wang
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  3. Cheng-Gang Gu
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  4. Yin-Ping Zhang
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  5. Zong-Zheng Yang
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  6. Xiao-Wei Wu
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  7. Xin Jiang
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Correspondence to Fang Wang or Xin Jiang.

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Wu, ZG., Wang, F., Gu, CG. et al. Aquamicrobium terrae sp. nov., isolated from the polluted soil near a chemical factory. Antonie van Leeuwenhoek 105, 1131–1137 (2014). https://doi.org/10.1007/s10482-014-0174-8

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  • DOI: https://doi.org/10.1007/s10482-014-0174-8

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