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Cytisus villosus from Northeastern Algeria is nodulated by genetically diverse Bradyrhizobium strains

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Abstract

Fifty-one rhizobial strains isolated from root nodules of Cytisus villosus growing in Northeastern Algeria were characterized by genomic and phenotypic analyses. Isolates were grouped into sixteen different patterns by PCR-RAPD. The phylogenetic status of one representative isolate from each pattern was examined by multilocus sequence analyses of four housekeeping genes (16S rRNA, glnII, recA, and atpD) and one symbiotic gene (nodC). Analysis of 16S rRNA gene sequences showed that all the isolates belonged to the genus Bradyrhizobium. Phylogenetic analyses based on individual or concatenated genes glnII, recA, and atpD indicated that strains cluster in three distinct groups. Ten out of the sixteen strains grouped together with Bradyrhizobium japonicum, while a second group of four clustered with Bradyrhizobium canariense. The third group, represented by isolates CTS8 and CTS57, differed significantly from all other bradyrhizobia known to nodulate members of the Genisteae tribe. In contrast with core genes, sequences of the nodC symbiotic gene from all the examined strains form a homogeneous group within the genistearum symbiovar of Bradyrhizobium. All strains tested nodulated Lupinus angustifolius, Lupinus luteus, and Spartium junceum but not Glycine max. From these results, it is concluded that C. villosus CTS8 and CTS57 strains represent a new lineage within the Bradyrhizobium genus.

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Acknowledgments

H. Ahnia and Y. Bourebaba acknowledge AECID support for a fellowship at CBGP, Madrid. This work was supported by AECID (A1/038234/11 to TRA) and MICINN (CGL-26932 to JI). We thank A. Bautista for technical assistance.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Laboratoire d’Ecologie Microbienne, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000, Bejaia, Algeria

    Hadjira Ahnia, Farida Boulila, Abdelghani Boulila, Karima Boucheffa, Yasmina Bourebaba & Adouda Salmi

  2. Departamento de Biotecnología (ETSI Agrónomos), Centro de Biotecnología y Genómica de Plantas (CBGP), Campus de Montegancedo, Universidad Politécnica de Madrid, 28223, Pozuelo de Alarcón, Madrid, Spain

    David Durán, Juan Imperial, Tomás Ruiz-Argüeso & Luis Rey

  3. CSIC, Madrid, Spain

    Juan Imperial

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  1. Hadjira Ahnia
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Correspondence to Luis Rey.

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Ahnia, H., Boulila, F., Boulila, A. et al. Cytisus villosus from Northeastern Algeria is nodulated by genetically diverse Bradyrhizobium strains. Antonie van Leeuwenhoek 105, 1121–1129 (2014). https://doi.org/10.1007/s10482-014-0173-9

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