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Paenibacillus beijingensis sp. nov., a nitrogen-fixing species isolated from wheat rhizosphere soil

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An Erratum to this article was published on 11 January 2014

Abstract

A novel nitrogen-fixing bacterium, BJ-18T, was isolated from wheat rhizosphere soil. Strain BJ-18T was observed to be Gram-positive, facultatively anaerobic, motile and rod-shaped (0.4–0.9 μm × 2.0–2.9 μm). Phylogenetic analysis based on a partial nifH gene sequence and an assay for nitrogenase activity showed its nitrogen-fixing capacity. Phylogenetic analysis based on full 16S rRNA gene sequences suggested that strain BJ-18T is a member of the genus Paenibacillus. High similarity of 16S rRNA gene sequence was found between BJ-18T and Paenibacillus peoriae DSM 8320T (99.05 %), Paenibacillus jamilae DSM 13815T (98.86 %), Paenibacillus brasiliensis DSM 13188T (98.55 %), Paenibacillus polymyxa DSM 36T (98.74 %), Paenibacillus terrae DSM 15891T (97.99 %) and Paenibacillus kribbensis JCM 11465T (97.92 %), whereas the similarity was below 96.0 % between BJ-18T and the other Paenibacillus species. DNA–DNA relatedness between strain BJ-18T and P. peoriae DSM 8320T, P. jamilae DSM 13815T, P. brasiliensis DSM 13188T, P. polymyxa DSM 36T, P. kribbensis JCM 11465T and P. terrae DSM 15891T was determined to be 43.6 ± 2.7, 34.2 ± 5.3, 47.9 ± 6.6, 36.8 ± 3.5, 27.4 ± 4.3 and 23.6 ± 4.1 % respectively. The DNA G+C content of BJ-18T was determined to be 45.8 mol %. The major fatty acid was identified as anteiso-C15:0 (67.1 %). The polar lipids present in strain BJ-18T were identified as diphosphatidylglycerol, phosphatidyl methylethanolamine, phosphatidylethanolamine and phosphatidylglycerol. The phenotypic and genotypic characteristics, and DNA–DNA relatedness data, suggest that BJ-18T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus beijingensis sp. nov. (Type strain BJ-18T=DSM25425T=CGMCC 1.12045T) is proposed.

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Acknowledgments

We are grateful to Dr. R. Pukall’s generous help when the strain was deposited in DSMZ. This work was supported by the 863 High Technology Program (Grant No. 2011BAD11B03-03) and the National Natural Science Foundation of China (Grant No. 31070082).

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Authors and Affiliations

  1. State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, 100193, China

    Li-Ying Wang & San-Feng Chen

  2. College of Resources and Environmental Engineering, China Agricultural University, Beijing, 100193, China

    Ji Li

  3. Department of Molecular Biosciences and Bioengineering, University of Hawaii, Honolulu, HI, 96822, USA

    Qing X. Li

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  1. Li-Ying Wang
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  2. Ji Li
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  3. Qing X. Li
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  4. San-Feng Chen
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Correspondence to San-Feng Chen.

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Wang, LY., Li, J., Li, Q.X. et al. Paenibacillus beijingensis sp. nov., a nitrogen-fixing species isolated from wheat rhizosphere soil. Antonie van Leeuwenhoek 104, 675–683 (2013). https://doi.org/10.1007/s10482-013-9974-5

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