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Aliidiomarina sanyensis sp. nov., a hexabromocyclododecane assimilating bacterium from the pool of Spirulina platensis cultivation, Sanya, China

Antonie van Leeuwenhoek volume 104, pages 309–314 (2013)Cite this article

Abstract

A novel Gram-negative, rod shaped, motile, non-spore-forming, aerobic, brominated flame retardant hexabromocyclododecane-assimilating bacterium, designated strain GYP-17T, was isolated from a pool of marine Spirulina platensis cultivation, Sanya, China. Colonies on 1/10 strength of marine Glycerol Enriched Medium plates were circular, dark-brown, 1–2 mm in diameter, and with regular margins. Growth occurred at 10–45 °C, 1–10 % (w/v) NaCl and pH of 7–9. The polar lipids were composed of phosphatidylethanolamine, three unidentified phospholipids and one unidentified polar lipid. The major fatty acids were iso-C17:1ω9c/10-methyl-C16:0 (summed feature 9, 20.75 %), iso-C15:0 (17.70 %) and C16:0 (6.40 %). The major respiratory quinone was Q-8. The DNA G + C content of the type strain was 53.6 mol%. Phylogenetic analysis revealed that strain GYP-17T was a member of the genus Aliidiomarina and closely related to Aliidiomarina haloalkalitolerans with a 16S rDNA sequence similarity of 96.36 %. Results from the polyphasic taxonomy study support the conclusion that strain GYP-17T represents a novel Aliidiomarina species, for which the name Aliidiomarina sanyensis sp. nov. is proposed. The type strain of A. sanyensis is GYP-17T (=KCTC 32218T =LMG 27471T).

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Acknowledgments

This research was supported by the National Basic Research Program of China (No. 2010CB833801), the National Natural Science Foundation of China (No. 41206136), Funds for marine renewable energy (GHME2011SW04), Public science and technology research funds projects of ocean (201005031-5), Guangdong Province and Chinese Academy of Science cooperation Foundation (2012B091100276), Provincial Collaborative Foundation Project of Guangdong (9351007002000001).

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Authors and Affiliations

  1. Key Laboratory of Marine Bio-resources Sustainable Utilization (LMB-CAS) Guangdong Key Laboratory of Marine Materia Medica (LMMM-GD), South China sea Institute of Oceaonlogy, Chinese Academy of Sciences, Guangzhou, 510301, China

    Guanghua Wang, Hualian Wu, Xiaoyong Zhang, Hao Zhang, Xi Yang, Xinpeng Tian, Jie Li, Wenzhou Xiang & Xiang Li

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  1. Guanghua Wang
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  2. Hualian Wu
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  3. Xiaoyong Zhang
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  4. Hao Zhang
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  6. Xinpeng Tian
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  7. Jie Li
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  8. Wenzhou Xiang
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Correspondence to Wenzhou Xiang.

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Wang, G., Wu, H., Zhang, X. et al. Aliidiomarina sanyensis sp. nov., a hexabromocyclododecane assimilating bacterium from the pool of Spirulina platensis cultivation, Sanya, China. Antonie van Leeuwenhoek 104, 309–314 (2013). https://doi.org/10.1007/s10482-013-9949-6

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Keywords

  • Aliidiomarina sanyensis
  • 16S rDNA based phylogeny
  • Chemotaxonomy